Strain Data

Strain number		NIES-4100
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Eudorina sp.
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Refer to NIES-2734 and NIES-2735
Latitude / Longitude
Habitat (Isolation source)		Freshwater
History		< Nozaki, Hisayoshi
Isolator (Date of isolation)		Nozaki, Hisayoshi (2010-06-23)
Identified by		Nozaki, Hisayoshi
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2017 May]
Culture condition (Preculture condition)		Medium: AF-6 Temperature: 20 C Light intensity: 21 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information
Cell size (min - max)		6 - 22 μm
Organization		Flagellate
Characteristics		Mixotrophic ; Osmotrophic ; Phototaxis ; F1 clone of NIES-2734 x 2735 ; Heterothallic ; Anisogamous ; Mating type (male) ; Haploid life cycle ; Resting zygote forming
Other strain no.		Other strain no. : 2010-623-F1-E3
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-717	Eudorina elegans	Green alga	Freshwater (Pond soil)	Phototaxis ; Heterothallic
NIES-720	Eudorina elegans	Green alga	Freshwater (Lake water)	Phototaxis ; Heterothallic
NIES-721	Eudorina elegans	Green alga	Freshwater (Pond water)	Phototaxis ; Homothallic ; Monoecious
NIES-459	Eudorina illinoisensis	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Mating type (female) ; Crosses with NIES-460
NIES-351	Eudorina elegans	Green alga	Freshwater (Lake water)	Phototaxis ; Homothallic

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-460	Eudorina illinoisensis	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Mating type (male) ; Crosses with NIES-459
NIES-456	Eudorina elegans	Green alga	Freshwater (Moat water)	Phototaxis ; Heterothallic ; Mating type (male) ; Crosses with NIES-457

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3982	Yamagishiella unicocca	Green alga	Freshwater	Phototaxis ; Genome decoded strain (Hamaji et al. 2018) ; backcross strain of NIES-1861 x F1 clone (NIES-1861 x 1859) ; Heterothallic ; Isogamous ; Mating type (+) ; Resting spore-forming ; MID gene (−)
NIES-3983	Yamagishiella unicocca	Green alga	Freshwater	Phototaxis ; Genome decoded strain (Hamaji et al. 2018) ; backcross strain of NIES-1861 x F1 clone (NIES-1861 x 1859) ; Heterothallic ; Isogamous ; Mating type (−) ; Resting spore-forming ; MID gene (+)
NIES-3984	Eudorina sp.	Green alga	Freshwater	Mixotrophic ; Osmotrophic ; Phototaxis ; Genome decoded strain (Hamaji et al. 2018) ; F1 clone of NIES-2734 x 2735 ; Heterothallic ; Anisogamous ; Mating type (female) ; Haploid life cycle ; Resting zygote forming
NIES-4568	Eudorina sp.	Green alga	Freshwater (Zygote of NIES-4564 x 4565)	Mixotrophic ; Osmotrophic ; Phototaxis
NIES-3985	Eudorina sp.	Green alga	Freshwater	Mixotrophic ; Osmotrophic ; Phototaxis ; Genome decoded strain (Hamaji et al. 2018) ; F1 clone of NIES-2734 x 2735 ; Heterothallic ; Anisogamous ; Mating type (male) ; Haploid life cycle ; Resting zygote forming

Reference
Hamaji, T., Kawai-Toyooka, H., Uchimura, H., Suzuki, M., Noguchi, H., Minakuchi, Y., Toyoda, A., Fujiyama, A., Miyagishima, S., Umen, J. G., Nozaki, H. 2018 Anisogamy evolved with a reduced sexdetermining region in volvocine green algae. Communications Biology, 1, 17 (article ID). Strain(s): 3982, 3983, 3984, 3985, 4018, 4100 DOI: 10.1038/s42003-018-0019-5 Kawai-Toyooka, H., Ono, M., Hamaji, T., Nozaki, H., Hirono, M. 2025 Synergistic effects of proteinaceous pheromone and nitrogen starvation on male gametogenesis in the anisogamous volvocine alga Eudorina. PLoS One, 20, e0326066 (article ID). Strain(s): 2734, 2735, 4100, 4564, 4565, 4567, 4568 PubMed: 41270121 DOI: 10.1371/journal.pone.0326066

Reference

Hamaji, T., Kawai-Toyooka, H., Uchimura, H., Suzuki, M., Noguchi, H., Minakuchi, Y., Toyoda, A., Fujiyama, A., Miyagishima, S., Umen, J. G., Nozaki, H. 2018 Anisogamy evolved with a reduced sexdetermining region in volvocine green algae. Communications Biology, 1, 17 (article ID).
Strain(s): 3982, 3983, 3984, 3985, 4018, 4100
DOI: 10.1038/s42003-018-0019-5

Kawai-Toyooka, H., Ono, M., Hamaji, T., Nozaki, H., Hirono, M. 2025 Synergistic effects of proteinaceous pheromone and nitrogen starvation on male gametogenesis in the anisogamous volvocine alga Eudorina. PLoS One, 20, e0326066 (article ID).
Strain(s): 2734, 2735, 4100, 4564, 4565, 4567, 4568
PubMed: 41270121
DOI: 10.1371/journal.pone.0326066

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