Strain number NIES-4321  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Lake Inbanuma, Usuida, Sakura, Chiba, Japan (2016-06-25)  
Latitude / Longitude 35.741717 / 140.202283 
Habitat (Isolation source) Freshwater (Lake water)  
History < Tsuchikane, Yuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2016-06-27)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 1506 group I intron ( LC685248 )  
Cell size (min - max) 209 - 215 μm  
Organization Unicellular 
Characteristics Heterothallic ; Isogamous ; Mating group II-C ; Mating type (−) ; Crossing with NIES-4322 and NIES-261  
Other strain no. Other strain no. : Inba1-12  
Remarks Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-228
Closterium ehrenbergii Desmid   Freshwater (Paddy soil) Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229  
NIES-229
Closterium ehrenbergii Desmid   Freshwater (Paddy soil) Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-58
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (−) ; Mating group II A  
NIES-60
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (+) ; Mating group II B  
NIES-4322
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Lake water) Heterothallic ; Isogamous ; Mating group II-C ; Mating type (+) ; Crossing with NIES-4321  
NIES-63
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (−) ; Mating group II A  
NIES-4550
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Vegetative cell germinated from a zygospore) Homothallic ; Isogamou ; Genome decoded strain (Kawaguchi et al. 2023)  
Reference
Tsuchikane, Y., Hamaji, T., Ota, K., Kato, S. 2018 Establishment of a clonal culture of unicellular conjugating algae. J. Vis. Exp., 14, e57761 (article ID).
Strain(s): 4321 
PubMed: 30059030
DOI: 10.3791/57761

Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 535458596263646568261266643194320432145484550455145524581458245834584 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Watanabe, M., Kawaguchi, Y. W., Uehara, K., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2024 Diversity of genome size and chromosome number in homothallic and heterothallic strains of the Closterium peracerosum–strigosum–littorale complex (Desmidiales, Zygnematophyceae, Streptophyta). J. Phycol., 60, 654-667.
Keywords: Closterium; flow cytometry; genome size; heterothallism; homothallism; sexual reproduction
Strain(s): 5253545859606162636465676826126664319432043214322454845494550455145524581458245834584 
PubMed: 38678594
DOI: 10.1111/jpy.13457

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