Strain Data

Strain number		NIES-2135
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Leptolyngbya boryana (Gomont) Anagnostidis & Komárek
Synonym
Former name		Tolypothrix tenuis Kützing ex Bornet & Flahault
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		(1960-06-**)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Tsuzuki, Mikio (2005) < IAM (1994) < Ishikawa, Masako
Isolator (Date of isolation)		Ishikawa, Masako
Identified by		Re-identified by DNA analysis at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2013 Feb]
Culture condition (Preculture condition)		Medium: BG-11 (agar) Temperature: 20 C Light intensity: 5-9 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 5 M
Gene information		Whole-genome ( AP018203 ) , 16S rRNA ( LC215287 ) , Plasmid DNA ( AP018204-AP018206 )
Cell size (min - max)
Organization
Characteristics		Genome decoded strain
Other strain no.		Other collection strain no. : IAM M-287 (=M-110)
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2104	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance ; Genome decoded strain (Shimura et al. 2015)
NIES-30	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Freshwater (Paddy field)	Genome decoded strain (Zhu et al. 2017)
NIES-3755	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Soil)	Genome decoded strain (Hirose et al. 2016)
NIES-2118	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria
NIES-2103	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-4106	Pelatocladus sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Soil)	Nitrogen fixation ; Attached ; Genome decoded strain
NIES-230	Merismopedia tenuissima	Blue-green alga ; Cyanobacteria	Freshwater (Pond water)
NIES-267	Calothrix parasitica	Blue-green alga ; Cyanobacteria	Marine (Seaweed)	Endophyte in Nemalion (Rhodophyceae) ; Genome decoded strain
NIES-2130	Scytonema sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance ; Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain (Zhu et al. 2017)
NIES-4101	Calothrix sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Sediment)	Genome decoded strain

Reference
Hamana, K., Kobayashi, M., Yokota, A., Sekiguchi, H., Niitsu, M. 2008 Cellular polyamine profiles in cyanobacteria. Microbiol. Cult. Coll., 24, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine Strain(s): 26, 33, 36, 46, 206, 208, 230, 267, 268, 305, 361, 416, 433, 434, 481, 507, 597, 598, 931, 932, 1031, 2115, 2133, 2135 Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID). Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring Strain(s): 22, 25, 42, 843, 2135, 2481 DOI: 10.1016/j.rsma.2018.100485 Chernomor, O., Peters, L., Schneidewind, J., Loeschcke, A., Knieps-Grünhagen, E., Schmitz, F., von Lieres, E.,Kutta, R. J., Svensson, V., Jaeger, K., Drepper, T.,von Haeseler, A., Krauss, U. 2021 Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function. Mol. Biol. Evol., 38, 819-837. Keywords: light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis Strain(s): 981, 2135 PubMed: 32931580 DOI: 10.1093/molbev/msaa234 Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID). Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106 PubMed: 34677568 DOI: 10.1093/dnares/dsab024 Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611. Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074 PubMed: 35595516 DOI: 10.1093/jxb/erac219 Christodoulou, M., Dextro, R. B., Wahlsten, M., Oivanen, P., Fewer, D. P., Fiore, M. F. 2026 Sivoneniella epilithica gen. et sp. nov.,(Leptolyngbyaceae, Cyanobacteria), from Suomenlinna Fortress (UNESCO World Heritage Site), Finland. Eur. J. Phycol., 61, 79-98. Keywords: 16S–23S ITS; 16S rRNA; cyanobacteria taxonomy; epilithic; Finland; Sivoneniella Strain(s): 2135, 3755 DOI: 10.1080/09670262.2025.2592827

Reference

Hamana, K., Kobayashi, M., Yokota, A., Sekiguchi, H., Niitsu, M. 2008 Cellular polyamine profiles in cyanobacteria. Microbiol. Cult. Coll., 24, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 26, 33, 36, 46, 206, 208, 230, 267, 268, 305, 361, 416, 433, 434, 481, 507, 597, 598, 931, 932, 1031, 2115, 2133, 2135

Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID).
Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring
Strain(s): 22, 25, 42, 843, 2135, 2481
DOI: 10.1016/j.rsma.2018.100485

Chernomor, O., Peters, L., Schneidewind, J., Loeschcke, A., Knieps-Grünhagen, E., Schmitz, F., von Lieres, E.,Kutta, R. J., Svensson, V., Jaeger, K., Drepper, T.,von Haeseler, A., Krauss, U. 2021 Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function. Mol. Biol. Evol., 38, 819-837.
Keywords: light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis
Strain(s): 981, 2135
PubMed: 32931580
DOI: 10.1093/molbev/msaa234

Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID).
Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy
Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106
PubMed: 34677568
DOI: 10.1093/dnares/dsab024

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Christodoulou, M., Dextro, R. B., Wahlsten, M., Oivanen, P., Fewer, D. P., Fiore, M. F. 2026 Sivoneniella epilithica gen. et sp. nov.,(Leptolyngbyaceae, Cyanobacteria), from Suomenlinna Fortress (UNESCO World Heritage Site), Finland. Eur. J. Phycol., 61, 79-98.
Keywords: 16S–23S ITS; 16S rRNA; cyanobacteria taxonomy; epilithic; Finland; Sivoneniella
Strain(s): 2135, 3755
DOI: 10.1080/09670262.2025.2592827

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