Strain Data

Strain number		NIES-2280
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Tetradesmus obliquus (Turpin) M.J.Wynne
Synonym
Former name		Scenedesmus obliquus (Turpin) Kützing
Common name		Green alga
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Tsuzuki, Mikio (1988) < Oh-Hama, Takeshi < Senger, H. (1965) < Bishop, N. < Gaffron, H.
Isolator (Date of isolation)		Gaffron, H.
Identified by
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2020 Oct]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 3-5 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M
Gene information
Cell size (min - max)		5 - 10 μm
Organization
Characteristics		Starch production (Miyachi et al. 1986)
Other strain no.		Other collection strain no. : IAM C-538 Other strain no. : Gaffron D-3
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-92	Tetradesmus acuminatus	Green alga	Freshwater (Lake water)
NIES-93	Tetradesmus dimorphus	Green alga	Freshwater (Lake water)
NIES-119	Tetradesmus dimorphus	Green alga	Freshwater (Bog water)
NIES-2279	Tetradesmus obliquus	Green alga		Thermophilic (opt. 57℃（) ; Genome decoded strain

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-418	Astrephomene gubernaculifera	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Mating type (−) ; Crosses with NIES-419
NIES-35	Raphidocelis subcapitata	Green alga	Freshwater	Test strain for AGP ; Genome decoded strain (Suzuki et al. 2018)
NIES-2162	Parachlorella kessleri	Green alga	Freshwater	White mutant of NIES-2160
NIES-551	Cyanidium caldarium	Red alga		Acidophilic ; Floridean starch (Shimonaga et al. 2007)
NIES-2170	Chlorella vulgaris	Green alga		Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)

Reference
Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1982 ¹³C-NMR evidence for the pathway of chlorophyll biosynthesis in algae. Biochem. Biophys. Res. Commun., 105, 647-652. Strain(s): 2280 DOI: 10.1016/0006-291X(82)91483-8 Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240. Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280 DOI: 10.1111/j.1749-6632.1984.tb12341.x Oh-hama, T., Seto, H., Miyachi, S. 1985 ¹³C-Nuclear magnetic resonance studies of the biosynthesis of 5-aminolevulinic acid destined for chlorophyll formation in dark-grown Scenedesmus obliquus. Plant Sci., 42, 153-158. Keywords: biosynthesis of 5-aminolevulinic acid; chlorophyll biosynthesis; 13C-nuclear magnetic resonance; Scenedesmus obliquus Strain(s): 2280 DOI: 10.1016/0168-9452(85)90121-9 Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319. Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus Strain(s): 687, 2157, 2160, 2170, 2173, 2280 Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1983 ¹³C-NMR spectra of chlorophylls a and b in green algae biosynthetically enriched with [1-¹³C] glutamate. FEBS Lett., 153, 404-408. Keywords: 5-aminolevulinic acid; chlorophyll a; chlorophyll b; 13C-NMR; [1-13C]glutamic acid; Scenedesmus obliquus Strain(s): 2280 Oh-hama, T., Siebelt, F., Furihata, K., Seto, H., Miyachi, S., Ohmori, K. 1986 ³¹P-NMR studies on inorganic polyphosphates in microalgae. J. Phycol., 22, 485-490. Keywords: Anabaena cylindrica; Chlorella vulgaris 11h; inorganic polyphosphate; 13P nuclear magnetic resonance of algae Strain(s): 2280 DOI: 10.1111/j.1529-8817.1986.tb02492.x Kotzabasis, K., Schuering, M., Senger, H. 1989 Occurrence of protochlorophyll and its phototransformation to chlorophyll in mutant C-2A' of Scenedesmus obliquus. Physiol. Plant., 75, 221-226. Keywords: green algae; phototransformation; protochlorophyllide; protochlorophyllide reductase; Scenedesmus obliquus Strain(s): 2280 DOI: 10.1111/j.1399-3054.1989.tb06172.x Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116. Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection Strain(s): 18, 144, 250, 530, 551, 2138, 2160, 2258, 2280 PubMed: 18079144 DOI: 10.1093/pcp/pcm172 Shen, X.-F., Liu, J.-J., Chu, F.-F., Lam, P.K.S., Zeng, R.J. 2015 Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation. Appl. Energy, 158, 348-354. Keywords: Scenedesmus obliquus; Heterotrophic cultivation; Acetate; Nitrogen deficiency; Phosphorus; Biodiesel productivity Strain(s): 2280 DOI: 10.1016/j.apenergy.2015.08.057 Piligaeva, A.V., Sorokinaab, K.N., Shashkovab, M.V., Parmon, V.N. 2018 Screening and comparative metabolic profiling of high lipid content microalgae strains for application in wastewater treatment. Bioresour. Technol., 250, 538-547. Keywords: Microalgae; Wastewater; Metabolic profiling; Lipid metabolism Strain(s): 2164, 2280 PubMed: 29197777 DOI: 10.1016/j.biortech.2017.11.063 Shen, X-F., Hu, H., Ma, L-L., Lam, P. K. S., Yan, S-K., Zhou, S-B., Zeng, R. J. 2018 FAMEs production from Scenedesmus obliquus in autotrophic, heterotrophic and mixotrophic cultures under different nitrogen conditions. Environ. Sci.-Wat. Res. Technol., 4, 461-468. Strain(s): 2280 DOI: 10.1039/C7EW00470B Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133. Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578 PubMed: 30304548 DOI: 10.1111/jpy.12798 Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151. Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion Strain(s): 94, 227, 2152, 2173, 2195, 2280 PubMed: 30658785 DOI: 10.1016/j.carbpol.2018.12.057 Shen, X-F., Gao, L-J., Zhou, S-B., Huang, J-L., Wu, C-Z., Qin, Q-W., Zeng, R. J. 2020 High fatty acid productivity from Scenedesmus obliquus in heterotrophic cultivation with glucose and soybean processing wastewater via nitrogen and phosphorus regulation. Sci. Total Environ., , 134596 (article ID). Keywords: Scenedesmus obliquus; heterotrophic cultivation; soybean processing wastewater; nitrogen deficiency; phosphorus; biodiesel productivity Strain(s): 2280 PubMed: 31780158 DOI: 10.1016/j.scitotenv.2019.134596 Hashizume, M., Yoshida, M., Demura, M., Watanabe, M. M. 2020 Culture study on utilization of phosphite by green microalgae. J. Appl. Phycol., 32, 889–899. Keywords: Phosphite utilization; Chlorella vulgaris; Coccomyxa subellipsoidea Strain(s): 2166, 2170, 2280 DOI: 10.1007/s10811-020-02088-2 Nagarajan, D., Lee, D.-J., Chang, J. 2018 Heterotrophic microalgal cultivation. In Bioreactors for microbial biomass and energy conversion, Eds. by Liao Q., Chang J., Herrmann C., Xia A., Springer, Singapore, pp. 117-160. Strain(s): 144, 227, 2280 DOI: 10.1007/978-981-10-7677-0_4 Oliveira, C. Y. B., Oliveira, C. D. L., Prasad, R., Ong, H. C., Araujo, E. S., Shabnam, N., Gálvez, A. O. 2021 A multidisciplinary review of Tetradesmus obliquus: a microalga suitable for large-scale biomass production and emerging environmental applications. Rev. Aquac., 13, 1594-1618. Keywords: Acutodesmus obliquus; bibliometric analysis; biorefinery; renewable energy; Scenedesmus obliquus; wastewater treatment Strain(s): 2280 DOI: 10.1111/raq.12536 Shen, X. F., Xu, Y. P., Tong, X. Q., Huang, Q., Zhang, S., Gong, J., Chu, F. F., Zeng, R. J. 2022 The mechanism of carbon source utilization by microalgae when co-cultivated with photosynthetic bacteria. Bioresour. Technol., 365, 28152 (article ID). Keywords: Microalgae; Photosynthetic bacteria; FAME; iTRAQ; Carbon mechanism Strain(s): 227, 2168, 2280 PubMed: 36265788 DOI: 10.1016/j.biortech.2022.128152

Reference

Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1982 ¹³C-NMR evidence for the pathway of chlorophyll biosynthesis in algae. Biochem. Biophys. Res. Commun., 105, 647-652.
Strain(s): 2280
DOI: 10.1016/0006-291X(82)91483-8

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Oh-hama, T., Seto, H., Miyachi, S. 1985 ¹³C-Nuclear magnetic resonance studies of the biosynthesis of 5-aminolevulinic acid destined for chlorophyll formation in dark-grown Scenedesmus obliquus. Plant Sci., 42, 153-158.
Keywords: biosynthesis of 5-aminolevulinic acid; chlorophyll biosynthesis; 13C-nuclear magnetic resonance; Scenedesmus obliquus
Strain(s): 2280
DOI: 10.1016/0168-9452(85)90121-9

Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319.
Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus
Strain(s): 687, 2157, 2160, 2170, 2173, 2280

Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1983 ¹³C-NMR spectra of chlorophylls a and b in green algae biosynthetically enriched with [1-¹³C] glutamate. FEBS Lett., 153, 404-408.
Keywords: 5-aminolevulinic acid; chlorophyll a; chlorophyll b; 13C-NMR; [1-13C]glutamic acid; Scenedesmus obliquus
Strain(s): 2280

Oh-hama, T., Siebelt, F., Furihata, K., Seto, H., Miyachi, S., Ohmori, K. 1986 ³¹P-NMR studies on inorganic polyphosphates in microalgae. J. Phycol., 22, 485-490.
Keywords: Anabaena cylindrica; Chlorella vulgaris 11h; inorganic polyphosphate; 13P nuclear magnetic resonance of algae
Strain(s): 2280
DOI: 10.1111/j.1529-8817.1986.tb02492.x

Kotzabasis, K., Schuering, M., Senger, H. 1989 Occurrence of protochlorophyll and its phototransformation to chlorophyll in mutant C-2A' of Scenedesmus obliquus. Physiol. Plant., 75, 221-226.
Keywords: green algae; phototransformation; protochlorophyllide; protochlorophyllide reductase; Scenedesmus obliquus
Strain(s): 2280
DOI: 10.1111/j.1399-3054.1989.tb06172.x

Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116.
Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection
Strain(s): 18, 144, 250, 530, 551, 2138, 2160, 2258, 2280
PubMed: 18079144
DOI: 10.1093/pcp/pcm172

Shen, X.-F., Liu, J.-J., Chu, F.-F., Lam, P.K.S., Zeng, R.J. 2015 Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation. Appl. Energy, 158, 348-354.
Keywords: Scenedesmus obliquus; Heterotrophic cultivation; Acetate; Nitrogen deficiency; Phosphorus; Biodiesel productivity
Strain(s): 2280
DOI: 10.1016/j.apenergy.2015.08.057

Piligaeva, A.V., Sorokinaab, K.N., Shashkovab, M.V., Parmon, V.N. 2018 Screening and comparative metabolic profiling of high lipid content microalgae strains for application in wastewater treatment. Bioresour. Technol., 250, 538-547.
Keywords: Microalgae; Wastewater; Metabolic profiling; Lipid metabolism
Strain(s): 2164, 2280
PubMed: 29197777
DOI: 10.1016/j.biortech.2017.11.063

Shen, X-F., Hu, H., Ma, L-L., Lam, P. K. S., Yan, S-K., Zhou, S-B., Zeng, R. J. 2018 FAMEs production from Scenedesmus obliquus in autotrophic, heterotrophic and mixotrophic cultures under different nitrogen conditions. Environ. Sci.-Wat. Res. Technol., 4, 461-468.
Strain(s): 2280
DOI: 10.1039/C7EW00470B

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578
PubMed: 30304548
DOI: 10.1111/jpy.12798

Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion
Strain(s): 94, 227, 2152, 2173, 2195, 2280
PubMed: 30658785
DOI: 10.1016/j.carbpol.2018.12.057

Shen, X-F., Gao, L-J., Zhou, S-B., Huang, J-L., Wu, C-Z., Qin, Q-W., Zeng, R. J. 2020 High fatty acid productivity from Scenedesmus obliquus in heterotrophic cultivation with glucose and soybean processing wastewater via nitrogen and phosphorus regulation. Sci. Total Environ., , 134596 (article ID).
Keywords: Scenedesmus obliquus; heterotrophic cultivation; soybean processing wastewater; nitrogen deficiency; phosphorus; biodiesel productivity
Strain(s): 2280
PubMed: 31780158
DOI: 10.1016/j.scitotenv.2019.134596

Hashizume, M., Yoshida, M., Demura, M., Watanabe, M. M. 2020 Culture study on utilization of phosphite by green microalgae. J. Appl. Phycol., 32, 889–899.
Keywords: Phosphite utilization; Chlorella vulgaris; Coccomyxa subellipsoidea
Strain(s): 2166, 2170, 2280
DOI: 10.1007/s10811-020-02088-2

Nagarajan, D., Lee, D.-J., Chang, J. 2018 Heterotrophic microalgal cultivation. In Bioreactors for microbial biomass and energy conversion, Eds. by Liao Q., Chang J., Herrmann C., Xia A., Springer, Singapore, pp. 117-160.
Strain(s): 144, 227, 2280
DOI: 10.1007/978-981-10-7677-0_4

Oliveira, C. Y. B., Oliveira, C. D. L., Prasad, R., Ong, H. C., Araujo, E. S., Shabnam, N., Gálvez, A. O. 2021 A multidisciplinary review of Tetradesmus obliquus: a microalga suitable for large-scale biomass production and emerging environmental applications. Rev. Aquac., 13, 1594-1618.
Keywords: Acutodesmus obliquus; bibliometric analysis; biorefinery; renewable energy; Scenedesmus obliquus; wastewater treatment
Strain(s): 2280
DOI: 10.1111/raq.12536

Shen, X. F., Xu, Y. P., Tong, X. Q., Huang, Q., Zhang, S., Gong, J., Chu, F. F., Zeng, R. J. 2022 The mechanism of carbon source utilization by microalgae when co-cultivated with photosynthetic bacteria. Bioresour. Technol., 365, 28152 (article ID).
Keywords: Microalgae; Photosynthetic bacteria; FAME; iTRAQ; Carbon mechanism
Strain(s): 227, 2168, 2280
PubMed: 36265788
DOI: 10.1016/j.biortech.2022.128152

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