Strain Data

Strain number		NIES-123
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Rusalka fusiformis (Matvienko) Nakada
Synonym		Chlorogonium fusiforme Matvienko
Former name		Chlorogonium metamorphum Skuja (in IAM)
Common name		Green alga
Locality (Date of collection)		Lake Shinsen-numa, Niseko, Hokkaido, Japan (1964-07-30)
Latitude / Longitude		42.904268 / 140.592828
Habitat (Isolation source)		Freshwater
History		< IAM (1983);
Isolator (Date of isolation)		Ichimura, Terunobu (1964-07-30)
Identified by		Ichimura, Terunobu; Nozaki, Hisayoshi; Nakada, Takashi (Reidentify)
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: AF-6 Temperature: 20 (25) C Light intensity: 6-8 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M (14 D)
Gene information		18S rRNA ( AB360750 ) , atpB ( AB084329 ) , rbcL ( AB010242 ) , psaB ( AB084370 )
Cell size (min - max)		- 18 μm
Organization		Unicellular; Flagellate
Characteristics		Homothallic ; Authentic strain ; Type specimen (NIES-50007, Epitype)
Other strain no.		Other collection strain no. : IAM C-349 (=C-569) Other strain no. : MKF-14
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-514	Planctonema lauterbornii	Green alga	Freshwater (Lake water)
NIES-295	Hydrodictyon reticulatum	Green alga	Freshwater (Paddy soil)	Homothallic
NIES-108	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; ST14 (Tanabe et al. 2007)
NIES-968	Chlamydomonas kuwadae	Green alga	Freshwater (Lake water)	Phototaxis
NIES-215	Pediastrum simplex	Green alga	Freshwater (Lake water)

Reference
Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490. Keywords: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales Strain(s): 123, 446 Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037. Keywords: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales Strain(s): 123, 439, 446, 692, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761 DOI: 10.1046/j.1529-8817.1998.341024.x Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen. nov. and Rusalka gen.nov. J. Phycol., 44, 751-760. Keywords: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales Strain(s): 123, 439, 692, 751, 755, 761, 1357, 1359, 1360, 1869, 4125 DOI: 10.1111/j.1529-8817.2008.00525.x Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348. Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny Strain(s): 123, 144, 421, 428, 432, 439, 446, 567, 569, 571, 656, 861, 887, 968, 1048, 2293, 2564, 4124, 4125 PubMed: 26595722 DOI: 10.1111/jeu.12283

Reference

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490.
Keywords: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales
Strain(s): 123, 446

Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037.
Keywords: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales
Strain(s): 123, 439, 446, 692, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761
DOI: 10.1046/j.1529-8817.1998.341024.x

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen. nov. and Rusalka gen.nov. J. Phycol., 44, 751-760.
Keywords: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 123, 439, 692, 751, 755, 761, 1357, 1359, 1360, 1869, 4125
DOI: 10.1111/j.1529-8817.2008.00525.x

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 123, 144, 421, 428, 432, 439, 446, 567, 569, 571, 656, 861, 887, 968, 1048, 2293, 2564, 4124, 4125
PubMed: 26595722
DOI: 10.1111/jeu.12283

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.