Strain Data

Strain number		NIES-995
Phylum		Streptophyta
Class		Mesostigmatophyceae
Scientific name		Mesostigma viride Lauterborn
Synonym
Former name
Common name
Locality (Date of collection)		Hojo-ohike Pond, Hojo, Tsukuba Ibaraki, Japan (1995-11-01)
Latitude / Longitude		36.175543 / 140.103682
Habitat (Isolation source)		Freshwater (Pond water)
History		< Miyamura, Shin-ichi
Isolator (Date of isolation)		Watanabe, Satoru (1995-11-01)
Identified by		Watanabe, Satoru
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Jan]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 10-17 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( KJ808699 KJ808700 ) , 18S rRNA ( KJ808698 ) , CAO ( AB453277 ) , CO1 ( HQ668015 ) , EF-3 ( HQ668017 ) , GAPDH ( HQ668011 ) , L12 ( HQ668018 ) , nitrate transporter gene ( HQ668016 ) , transcriptome ( GBSK00000000 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Phototaxis ; Cells larger than normal strains
Other strain no.		Other strain no. : Hojo1
Remarks		Unstable; Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-296	Mesostigma viride		Freshwater (Lake water)	Genome decoded strain (Lemieux, C. et al. 2000, Turmel, M. et al. 2002b, Liang et al. 2020)
NIES-476	Mesostigma viride		Freshwater (Pond sediment)	Heterothallic ; Mating type (−)
NIES-475	Mesostigma viride		Freshwater (Pond sediment)	Heterothallic ; Mating type (+)
NIES-477	Mesostigma viride		Freshwater (Pond sediment)	Heterothallic ; Mating type (−) ; Genome decoded strain (Glass, S. E. et al. 2023)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2176	Auxenochlorella protothecoides	Green alga	(The freshwater sponge Spongilla fluviatalis)
NIES-1846	Spumella sp.		Freshwater (Pond water)	Phagotrophic ; Planktonic ; Cosmopolitan ; Phagotrophic ; Genome decoded strain (Dorrell et al. 2019)
NIES-24	Nostoc commune	Blue-green alga ; Cyanobacteria	Terrestrial (Plant (The moss Cavicularia densa))	Drought tolerance ; Epiphytic
NIES-2377	Ovulinata parva		Marine (Seawater)	Heterotrophic ; Phagotrophic ; Benthic
NIES-1862	Giraudyopsis sp.		Marine (Seawater)

Reference
Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89. Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860 PubMed: 24346654 DOI: 10.1007/s10265-013-0596-9 Ju, C., Van de Poel, B., Cooper,E.D., Thierer, J., Gibbons, T., Delwiche, C.F., Chang, C. 2015 Conservation of ethylene as a plant hormone over 450 million years of evolution. Nature Plants, 1, 14004 (article ID). Strain(s): 995 PubMed: 27246051 DOI: 10.1038/nplants.2014.4 Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229. Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome Strain(s): 296, 475, 995 PubMed: 27037587 DOI: 10.1111/jpy.12385 Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000. Strain(s): 254, 296, 626, 995 PubMed: 33649548 DOI: 10.1038/s41396-021-00899-w Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294. Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860 PubMed: 35672454 DOI: 10.1038/s41396-022-01264-1

Reference

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860
PubMed: 24346654
DOI: 10.1007/s10265-013-0596-9

Ju, C., Van de Poel, B., Cooper,E.D., Thierer, J., Gibbons, T., Delwiche, C.F., Chang, C. 2015 Conservation of ethylene as a plant hormone over 450 million years of evolution. Nature Plants, 1, 14004 (article ID).
Strain(s): 995
PubMed: 27246051
DOI: 10.1038/nplants.2014.4

Yang, A., Narechania, A., Kim, E. 2016 Rickettsial endosymbiont in the “early‐diverging” streptophyte green alga Mesostigma viride. J. Phycol., 52, 219-229.
Keywords: endosymbiosis; green algae; Mesostigma ; Rickettsiales; symbiosis; transcriptome
Strain(s): 296, 475, 995
PubMed: 27037587
DOI: 10.1111/jpy.12385

Bock, N. A., Charvet, S., Burns, J., Gyaltshen, Y., Rozenberg, A., Duhamel ,S., Kim, E. 2021 Experimental identification and in silico prediction of bacterivory in green algae. ISME J., 15, 1987-2000.
Strain(s): 254, 296, 626, 995
PubMed: 33649548
DOI: 10.1038/s41396-021-00899-w

Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294.
Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860
PubMed: 35672454
DOI: 10.1038/s41396-022-01264-1

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