Strain Data

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Strain number		NIES-47
Phylum		Euglenozoa
Class		Euglenophyceae
Scientific name		Euglena gracilis G.A.Klebs
Synonym
Former name
Common name
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (1983);
Isolator (Date of isolation)
Identified by
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: HUT (semi-solid) Temperature: 20 (25) C Light intensity: 6-8 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M (14 D)
Gene information
Cell size (min - max)		10 - 50 μm
Organization		Unicellular; Flagellate
Characteristics		Material for Vitamin B₁₂bioassay
Other strain no.		Other collection strain no. : IAM E-3
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-286	Euglena mutabilis		Freshwater (Sediment)	Bioindicator
NIES-253	Euglena clara		Freshwater (River sediment)
NIES-48	Euglena gracilis			Material for Vitamin B₁₂bioassay
NIES-3745	Euglena anabaena		Freshwater (Pond water)
NIES-49	Euglena gracilis

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2149	Euglena viridis

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-30	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Freshwater (Paddy field)	Genome decoded strain (Zhu et al. 2017)
NIES-527	Spirulina subsalsa	Blue-green alga ; Cyanobacteria	Marine
NIES-28	Desmonostoc sp.	Blue-green alga ; Cyanobacteria
NIES-26	Nostoc minutum	Blue-green alga ; Cyanobacteria	Terrestrial	Chromatic adaptation
NIES-450	Closterium praelongum	Desmid	Freshwater (Pond sediment)	Homothallic

Reference
Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese). Strain(s): 47, 48, 49 Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292. Strain(s): 19, 21, 22, 23, 24, 25, 26, 28, 29, 37, 47, 49, 226, 227 Kuroiwa, T., Suzuki, T., Ogawa, K., Kawano, S. 1981 The chloroplast nucleus: Distribution, number, size and shape, and a model for the multiplication of the chloroplast genome during chloroplast development. Plant Cell Physiol., 22, 381-396. Keywords: chloroplast nuclei; classification (chloroplast); development (Chloroplast); division cycle (chloroplast) Strain(s): 47, 2572 DOI: 10.1093/oxfordjournals.pcp.a076181 Tie, J., Uchigasaki, S., Haseba, T., Ohno, Y., Isahai, I., Oshida, S. 2010 Direct and rapid PCR amplification using digested tissues for the diagnosis of drowning. Electrophoresis, 31, 2411-2415. Keywords: diagnosis; direct PCR amplification; drowning; human tissues; phytoplankton Strain(s): 47 PubMed: 20564264 DOI: 10.1002/elps.200900754 Nakamura, M., Yamagiwa, Y., HigashiuraA, N., Tokino, S., Maeda, T. 2018 Fundamental study on application of the newly isolated Euglena to wastewater treatment. Jpn. J. Water Treat. Biol., 54, 39-46 (in Japanese with English summary). Keywords: Euglena; growth rate; wastewater treatmen Strain(s): 47, 48, 49 DOI: 10.2521/jswtb.54.39 Demura, M. 2020 Biomass productivity and paramylon content of 3 strains of Euglena gracilis. Bull. Fac. Agr., Saga Univ., 105, 1-7 (in Japanese with English summary). Keywords: Euglena; paramylon; biomass productivity; cost Strain(s): 47, 48, 49 Strain(s): 24, 47, 144, 2146, 2160 Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62. Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392 DOI: 10.60369/microresys.38.2_51 Kang, S., Kim, K.-H., Chung, N., Li, Z. 2023 A new omega-3 rich euglenoid Eutreptiella sp. from the Korean coastal waters. Algal Res., 75, 103296 (article ID). Keywords: Euglenoids; Eutreptiella sp. KCTC 19016P; Fatty acids; Microalgae; Phytoplankton; Phylogenetic analysis Strain(s): 47, 381, 2305 DOI: 10.1016/j.algal.2023.103296

Reference

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese).
Strain(s): 47, 48, 49

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 19, 21, 22, 23, 24, 25, 26, 28, 29, 37, 47, 49, 226, 227

Kuroiwa, T., Suzuki, T., Ogawa, K., Kawano, S. 1981 The chloroplast nucleus: Distribution, number, size and shape, and a model for the multiplication of the chloroplast genome during chloroplast development. Plant Cell Physiol., 22, 381-396.
Keywords: chloroplast nuclei; classification (chloroplast); development (Chloroplast); division cycle (chloroplast)
Strain(s): 47, 2572
DOI: 10.1093/oxfordjournals.pcp.a076181

Tie, J., Uchigasaki, S., Haseba, T., Ohno, Y., Isahai, I., Oshida, S. 2010 Direct and rapid PCR amplification using digested tissues for the diagnosis of drowning. Electrophoresis, 31, 2411-2415.
Keywords: diagnosis; direct PCR amplification; drowning; human tissues; phytoplankton
Strain(s): 47
PubMed: 20564264
DOI: 10.1002/elps.200900754

Nakamura, M., Yamagiwa, Y., HigashiuraA, N., Tokino, S., Maeda, T. 2018 Fundamental study on application of the newly isolated Euglena to wastewater treatment. Jpn. J. Water Treat. Biol., 54, 39-46 (in Japanese with English summary).
Keywords: Euglena; growth rate; wastewater treatmen
Strain(s): 47, 48, 49
DOI: 10.2521/jswtb.54.39

Demura, M. 2020 Biomass productivity and paramylon content of 3 strains of Euglena gracilis. Bull. Fac. Agr., Saga Univ., 105, 1-7 (in Japanese with English summary).
Keywords: Euglena; paramylon; biomass productivity; cost
Strain(s): 47, 48, 49

Strain(s): 24, 47, 144, 2146, 2160

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392
DOI: 10.60369/microresys.38.2_51

Kang, S., Kim, K.-H., Chung, N., Li, Z. 2023 A new omega-3 rich euglenoid Eutreptiella sp. from the Korean coastal waters. Algal Res., 75, 103296 (article ID).
Keywords: Euglenoids; Eutreptiella sp. KCTC 19016P; Fatty acids; Microalgae; Phytoplankton; Phylogenetic analysis
Strain(s): 47, 381, 2305
DOI: 10.1016/j.algal.2023.103296

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