Strain Data

A big image opens when the thumbnail is clicked.(nies-0049.jpg)

Strain number		NIES-49
Phylum		Euglenozoa
Class		Euglenophyceae
Scientific name		Euglena gracilis G.A.Klebs
Synonym
Former name		Euglena gracilis var. bacillaris Pringsheim
Common name
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (1983);
Isolator (Date of isolation)
Identified by
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: HUT Temperature: 20 (25) C Light intensity: 6-8 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 45 D (14 D)
Gene information
Cell size (min - max)		40 - 50 μm
Organization		Unicellular; Flagellate
Characteristics
Other strain no.		Other collection strain no. : IAM E-2 (=E-10)
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-286	Euglena mutabilis		Freshwater (Sediment)	Bioindicator
NIES-253	Euglena clara		Freshwater (River sediment)
NIES-47	Euglena gracilis			Material for Vitamin B₁₂bioassay
NIES-48	Euglena gracilis			Material for Vitamin B₁₂bioassay
NIES-3745	Euglena anabaena		Freshwater (Pond water)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2149	Euglena viridis

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-45	Planktothrix spiroides	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko)
NIES-37	Tolypothrix tenuis	Blue-green alga ; Cyanobacteria		Heterotrophic ; Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Chromatic adaptation ; Material for studying on phycobilin production ; Material for studying on phycobilin production ; Genome decoded strain
NIES-33	Oscillatoria tenuis	Blue-green alga ; Cyanobacteria
NIES-23	Anabaena variabilis	Blue-green alga ; Cyanobacteria		Genome decoded strain
NIES-309	Pleurotaenium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (+) ; Crosses with NIES-310

Reference
Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Iwahori, K., Miyata, N., Umeda, Y., Aoyama, K., Shimoi, K., Kinae, N. 1999 Applicability of environmental microorganisms to alkaline single cell gel electrophoresis (comet) assay. Jpn. J. Water Treat. Biol., 35, 261-270 (in Japanese with English summary). Keywords: comet assay; DNA damage; environmental microorganisms; Euglena gracilis; cell lysis; unwinding; electrophoresis; SDS; γ-irradiation Strain(s): 49, 122, 403 Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese). Strain(s): 47, 48, 49 Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292. Strain(s): 19, 21, 22, 23, 24, 25, 26, 28, 29, 37, 47, 49, 226, 227 Nishimura, M. & Huzisige, H. 1959 Studies on the chlorophyll formation in Euglena gracilis with special reference to the action spectrum of the process. J. Biochem., 46, 225-234. Strain(s): 49 Aoyama, K., Miyata, N., Iwahori, K. 2005 Evalution of DNA damaging potential in landfill leachates by comet assay using Euglena gracilis. Jpn. J. Water Treat. Biol., 41, 73-81. Keywords: comet assay; Euglena gracilis; landfill leachate; umu test Strain(s): 49 DOI: 10.2521/jswtb.41.73 Nakamura, M., Yamagiwa, Y., HigashiuraA, N., Tokino, S., Maeda, T. 2018 Fundamental study on application of the newly isolated Euglena to wastewater treatment. Jpn. J. Water Treat. Biol., 54, 39-46 (in Japanese with English summary). Keywords: Euglena; growth rate; wastewater treatmen Strain(s): 47, 48, 49 DOI: 10.2521/jswtb.54.39 Aoyama, K., Iwahori, K., Miyata, N. 2003 Application of Euglena gracilis cells to comet assay: evaluation of DNA damage and repair. Mutat. Res. Genet. Toxicol. Environ. , 538, 155-162. Keywords: Comet assay; Single-cell gel electrophoresis; Euglena gracilis; DNA damage; DNA repair Strain(s): 49 DOI: 10.1016/S1383-5718(03)00113-X Iwahori, K., Miyata, N., Yamamoto, Y., Sone, D., Aoyama, K. 2004 Normalization of DNA damage indices in comet assay for comparing with various mutagenicity tests. Jpn. J. Water Treat. Biol., 40, 63-70. Keywords: comet assay; tail moment; normalization; mutagenicity test Strain(s): 49 DOI: 10.2521/jswtb.40.63 Okamoto, Y., Haraguchi, Y., Sawamura, N., Asahi, T., Shimizu, T. 2020 Mammalian cell cultivation using nutrients extracted from microalgae. Biotechnol. Prog., 36, e2941 (article ID). Keywords: acidic hydrolysis; amino acids; glucose; mammalian cell culture; microalgae; nutrients Strain(s): 27, 39, 49, 2170 PubMed: 31756286 DOI: 10.1002/btpr.2941 Demura, M. 2020 Biomass productivity and paramylon content of 3 strains of Euglena gracilis. Bull. Fac. Agr., Saga Univ., 105, 1-7 (in Japanese with English summary). Keywords: Euglena; paramylon; biomass productivity; cost Strain(s): 47, 48, 49 Horikoshi, S., Hidaka, T., Nishimura, F., Kishimoto, N. 2024 Development of separation and recovery technology of cultured Euglena gracilis using activated sludge for anaerobic digestion. Bioresour. Technol. Reports, 25, 101790 (article ID). Keywords: Methane fermentation; Sewage treatment plant; Waste activated sludge Strain(s): 49 DOI: 10.1016/j.biteb.2024.101790

Reference

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Iwahori, K., Miyata, N., Umeda, Y., Aoyama, K., Shimoi, K., Kinae, N. 1999 Applicability of environmental microorganisms to alkaline single cell gel electrophoresis (comet) assay. Jpn. J. Water Treat. Biol., 35, 261-270 (in Japanese with English summary).
Keywords: comet assay; DNA damage; environmental microorganisms; Euglena gracilis; cell lysis; unwinding; electrophoresis; SDS; γ-irradiation
Strain(s): 49, 122, 403

Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese).
Strain(s): 47, 48, 49

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 19, 21, 22, 23, 24, 25, 26, 28, 29, 37, 47, 49, 226, 227

Nishimura, M. & Huzisige, H. 1959 Studies on the chlorophyll formation in Euglena gracilis with special reference to the action spectrum of the process. J. Biochem., 46, 225-234.
Strain(s): 49

Aoyama, K., Miyata, N., Iwahori, K. 2005 Evalution of DNA damaging potential in landfill leachates by comet assay using Euglena gracilis. Jpn. J. Water Treat. Biol., 41, 73-81.
Keywords: comet assay; Euglena gracilis; landfill leachate; umu test
Strain(s): 49
DOI: 10.2521/jswtb.41.73

Nakamura, M., Yamagiwa, Y., HigashiuraA, N., Tokino, S., Maeda, T. 2018 Fundamental study on application of the newly isolated Euglena to wastewater treatment. Jpn. J. Water Treat. Biol., 54, 39-46 (in Japanese with English summary).
Keywords: Euglena; growth rate; wastewater treatmen
Strain(s): 47, 48, 49
DOI: 10.2521/jswtb.54.39

Aoyama, K., Iwahori, K., Miyata, N. 2003 Application of Euglena gracilis cells to comet assay: evaluation of DNA damage and repair. Mutat. Res. Genet. Toxicol. Environ. , 538, 155-162.
Keywords: Comet assay; Single-cell gel electrophoresis; Euglena gracilis; DNA damage; DNA repair
Strain(s): 49
DOI: 10.1016/S1383-5718(03)00113-X

Iwahori, K., Miyata, N., Yamamoto, Y., Sone, D., Aoyama, K. 2004 Normalization of DNA damage indices in comet assay for comparing with various mutagenicity tests. Jpn. J. Water Treat. Biol., 40, 63-70.
Keywords: comet assay; tail moment; normalization; mutagenicity test
Strain(s): 49
DOI: 10.2521/jswtb.40.63

Okamoto, Y., Haraguchi, Y., Sawamura, N., Asahi, T., Shimizu, T. 2020 Mammalian cell cultivation using nutrients extracted from microalgae. Biotechnol. Prog., 36, e2941 (article ID).
Keywords: acidic hydrolysis; amino acids; glucose; mammalian cell culture; microalgae; nutrients
Strain(s): 27, 39, 49, 2170
PubMed: 31756286
DOI: 10.1002/btpr.2941

Demura, M. 2020 Biomass productivity and paramylon content of 3 strains of Euglena gracilis. Bull. Fac. Agr., Saga Univ., 105, 1-7 (in Japanese with English summary).
Keywords: Euglena; paramylon; biomass productivity; cost
Strain(s): 47, 48, 49

Horikoshi, S., Hidaka, T., Nishimura, F., Kishimoto, N. 2024 Development of separation and recovery technology of cultured Euglena gracilis using activated sludge for anaerobic digestion. Bioresour. Technol. Reports, 25, 101790 (article ID).
Keywords: Methane fermentation; Sewage treatment plant; Waste activated sludge
Strain(s): 49
DOI: 10.1016/j.biteb.2024.101790

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.