Strain Data

Strain number		NIES-137
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Echinosphaeridium nordstedtii Lemmermann
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Lake Kasumigaura, Ibaraki, Japan (1983-08-23)
Latitude / Longitude		36.081204 / 140.214193
Habitat (Isolation source)		Freshwater (Lake water)
History		< Kasai, Fumie
Isolator (Date of isolation)		Kasai, Fumie (1983-08-24)
Identified by		Watanabe, Masayuki
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic[2016 Mar]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 (25) C Light intensity: 2 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (14 D)
Gene information		18S rRNA ( LC192135 )
Cell size (min - max)
Organization
Characteristics
Other strain no.		Other strain no. : F-56-3
Remarks		Cryopreserved
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-301	Pediastrum boryanum	Green alga	Freshwater (Lake water)
NIES-650	Gonium quadratum	Green alga	Freshwater	Phototaxis ; Heterothallic ; Isogamous ; Mating type (−) ; F1 clone of NIES-652 x 653 ; Sister clone to NIES-647, 648 and 649 from one zygote
NIES-92	Tetradesmus acuminatus	Green alga	Freshwater (Lake water)
NIES-694	Volvox aureus	Green alga ; Volvocine alga	Freshwater (Stream water)	Other water bloom ; Phototaxis ; Homothallic ; Dioecious ; Oogamy
NIES-351	Eudorina elegans	Green alga	Freshwater (Lake water)	Phototaxis ; Homothallic

Reference
Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83. Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298 DOI: 10.1016/0269-7491(93)90180-V Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Reference

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298
DOI: 10.1016/0269-7491(93)90180-V

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

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