Strain Data

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Strain number		NIES-257
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Hafniomonas laevis Nakada, Suda & Nozaki
Synonym
Former name		Hafniomonas montana (Geitler) Ettl et Moestrup
Common name		Green alga
Locality (Date of collection)		inside NIES, Onogawa, Tsukuba, Ibaraki, Japan (1983-10-30)
Latitude / Longitude		36.048471 / 140.117609
Habitat (Isolation source)		Freshwater (Pool water)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1983-10-30)
Identified by		Inouye, Isao; Nakada, et al. (Reidentify)
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 20 (20) C Light intensity: 6-8 (10-17) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M (14 D)
Gene information		Plastid genome ( KT625415 ) , 18S rRNA ( AB101517 ) , atpB ( AB101504 ) , rbcL ( AB101510 ) , psaB ( AB101515 )
Cell size (min - max)		15 - 17 μm
Organization		Unicellular; Flagellate
Characteristics		Authentic strain (Nakada et al. 2007) ; Genome decoded strain (Lemieux et al. 2015)
Other strain no.		Other strain no. : OUT-5
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-656	Hafniomonas montana	Green alga	Freshwater (Pond sediment)	Authentic strain, Epitype (Nakada et al. 2007)
NIES-1715	Hafniomonas reticulata	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Planktonic ; Cosmopolitan ; Authentic strain, Epitype (Nakada et al. 2007) ; Resting spore-forming

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1718	Hafniomonas reticulata	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Planktonic ; Cosmopolitan ; Resting spore-forming
NIES-1841	Hafniomonas sp.	Green alga	Freshwater (Pond water)	Planktonic
NIES-1714	Hafniomonas conica	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Planktonic ; Cosmopolitan
NIES-1717	Hafniomonas reticulata	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Cosmopolitan ; Resting spore-forming

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-648	Gonium quadratum	Green alga	Freshwater	Phototaxis ; Heterothallic ; Isogamous ; Mating type (+) ; F1 clone of NIES-652 x 653 ; Sister clone to NIES-647, 649 and 650 from one zygote
NIES-448	Closterium acerosum	Desmid	Freshwater
NIES-633	Carteria eugametos	Green alga	Freshwater (Paddy soil)	Homothallic ; Isogamous
NIES-458	Eudorina elegans	Green alga	Freshwater (Paddy soil)	Phototaxis ; Homothallic ; Monoecious
NIES-1716	Hafniomonas reticulata	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Planktonic ; Cosmopolitan ; Resting spore-forming

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Misumi, O., Kuroiwa, T. 2003 Phylogeny of the quadriflagellate Volvocales (Chlorophyceae) based on chloroplast multigene sequences. Mol. Phylogenet. Evol., 29, 58-66. Strain(s): 257, 656 PubMed: 12967607 DOI: 10.1016/S1055-7903(03)00089-7 Suda, S. & Watanabe, M. M. 1995 Notes on microalgae in Japan (2). Hafniomonas montana (Geitler) Ettl et Moestrup (Chlorophyceae, Dunaliellales). Jpn. J. Phycol. (Sôrui), 43, 40-41 (in Japanese with English title). Strain(s): 257 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579 Nakazawa, A. & Nozaki, H. 2004 Phylogenetic analysis of the Tetrasporalean genus Asterococcus (Chlorophyceae) based on 18S ribosomal RNA gene sequences. J. Jpn. Bot., 79, 255-261. Keywords: 18S rRNA gene; Asterococcus; Palmellopsidaceae; phylogeny; Tetrasporales Strain(s): 257, 656 Nakada, T., Suda, S., Nozaki, H. 2007 A taxonomic study of Hafniomonas (Chlorophyceae) based on a comparative examination of cultured material. J. Phycol., 43, 397-411. Keywords: Chlorophyceae; Hafniomonas; morphology; new species; taxonomy Strain(s): 257, 656, 1714, 1715, 1716, 1717, 1718, 1720 DOI: 10.1111/j.1529-8817.2007.00328.x Lemieux, C., Vincent, A. T., Labarre, A., Otis, C., Turmel M. 2015 Chloroplast phylogenomic analysis of chlorophyte green algae identifies a novel lineage sister to the Sphaeropleales (Chlorophyceae). BMC Evol. Biol., 15, 264 (article ID). Keywords: Chlorophyceae; Chlamydomonadales; Golenkiniaceae; Treubarinia, Jenufa genus; Plastid genome; Flagellar apparatus Strain(s): 257, 425 PubMed: 26620802 DOI: 10.1186/s12862-015-0544-5 Sausen, N., Malavasi, V., Melkonian, M. 2018 Molecular phylogeny, systematics and revision of the type species of Lobomonas, L. francei,(Volvocales, Chlorophyta) and closely related taxa. J. Phycol., 54, 198-214. Keywords: crystalline glycoprotein lattice; CWClade; ITS2; L. panduriformis; rbcL; Reinhardtinia; scanning electron microscopy; SSU nrRNA Strain(s): 257, 418, 456, 474, 541, 566, 577, 666, 735, 879, 880, 882, 883, 1362, 1708, 1858, 2567, 3923 PubMed: 29278416 DOI: 10.1111/jpy.12615 Chen, Y., Xiong, Q., Dai, Q., Liu, G. 2025 Six newly sequenced chloroplast genomes from quadriflagellate Chlamydomonadales (Chlorophyceae): Phylogeny and comparative genome analyses. Genome Biol. Evol., 17, evaf074 (article ID). Keywords: quadriflagellate chlamydomonadaleans; phylogenomics; Corbierea; Strain(s): 153, 257, 425, 432, 474, 566, 728, 875, 1363, 4017 PubMed: 40247659 DOI: 10.1093/gbe/evaf074

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Misumi, O., Kuroiwa, T. 2003 Phylogeny of the quadriflagellate Volvocales (Chlorophyceae) based on chloroplast multigene sequences. Mol. Phylogenet. Evol., 29, 58-66.
Strain(s): 257, 656
PubMed: 12967607
DOI: 10.1016/S1055-7903(03)00089-7

Suda, S. & Watanabe, M. M. 1995 Notes on microalgae in Japan (2). Hafniomonas montana (Geitler) Ettl et Moestrup (Chlorophyceae, Dunaliellales). Jpn. J. Phycol. (Sôrui), 43, 40-41 (in Japanese with English title).
Strain(s): 257

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Nakazawa, A. & Nozaki, H. 2004 Phylogenetic analysis of the Tetrasporalean genus Asterococcus (Chlorophyceae) based on 18S ribosomal RNA gene sequences. J. Jpn. Bot., 79, 255-261.
Keywords: 18S rRNA gene; Asterococcus; Palmellopsidaceae; phylogeny; Tetrasporales
Strain(s): 257, 656

Nakada, T., Suda, S., Nozaki, H. 2007 A taxonomic study of Hafniomonas (Chlorophyceae) based on a comparative examination of cultured material. J. Phycol., 43, 397-411.
Keywords: Chlorophyceae; Hafniomonas; morphology; new species; taxonomy
Strain(s): 257, 656, 1714, 1715, 1716, 1717, 1718, 1720
DOI: 10.1111/j.1529-8817.2007.00328.x

Lemieux, C., Vincent, A. T., Labarre, A., Otis, C., Turmel M. 2015 Chloroplast phylogenomic analysis of chlorophyte green algae identifies a novel lineage sister to the Sphaeropleales (Chlorophyceae). BMC Evol. Biol., 15, 264 (article ID).
Keywords: Chlorophyceae; Chlamydomonadales; Golenkiniaceae; Treubarinia, Jenufa genus; Plastid genome; Flagellar apparatus
Strain(s): 257, 425
PubMed: 26620802
DOI: 10.1186/s12862-015-0544-5

Sausen, N., Malavasi, V., Melkonian, M. 2018 Molecular phylogeny, systematics and revision of the type species of Lobomonas, L. francei,(Volvocales, Chlorophyta) and closely related taxa. J. Phycol., 54, 198-214.
Keywords: crystalline glycoprotein lattice; CWClade; ITS2; L. panduriformis; rbcL; Reinhardtinia; scanning electron microscopy; SSU nrRNA
Strain(s): 257, 418, 456, 474, 541, 566, 577, 666, 735, 879, 880, 882, 883, 1362, 1708, 1858, 2567, 3923
PubMed: 29278416
DOI: 10.1111/jpy.12615

Chen, Y., Xiong, Q., Dai, Q., Liu, G. 2025 Six newly sequenced chloroplast genomes from quadriflagellate Chlamydomonadales (Chlorophyceae): Phylogeny and comparative genome analyses. Genome Biol. Evol., 17, evaf074 (article ID).
Keywords: quadriflagellate chlamydomonadaleans; phylogenomics; Corbierea; Strain(s): 153, 257, 425, 432, 474, 566, 728, 875, 1363, 4017
PubMed: 40247659
DOI: 10.1093/gbe/evaf074

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