Strain Data

Strain number		NIES-5
Phylum		Heterokontophyta
Class		Raphidophyceae
Scientific name		Heterosigma akashiwo (Hada) Hada ex Y.Hara & Chihara
Synonym		Entomosigma akashiwo Y.Hada; Chattonella akashiwo (Y.Hada) Loeblich III 1979; Olisthodiscus carterae Hulburt 1965; Heterosigma inlandica Hada 1968; Chattonella inlandica (Y.Hada) Loeblich III & Fine 1977; Heterosigma carterae (Hulbert) F.J.R.Taylor 1992
Former name
Common name
Locality (Date of collection)		Gokasho Bay, Mie, Japan (1966--)
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< Iwasaki, Hideo
Isolator (Date of isolation)		Iwasaki, Hideo
Identified by		Hara, Yoshiaki
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 31 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( AB181953 ) , 16S-tRNA-Ile-tRNA-Ala-23S ( AB181957 ) , 28S rRNA ( AB217644 ) , CO1 ( AB334506 ) , rbcL ( AB334433 ) , rbcL, rbcS, cfxQ, psbA ( AB176660 )
Cell size (min - max)		10 - 15 μm
Organization		Unicellular; Flagellate
Characteristics		Red tide
Other strain no.		Other strain no. : GHE
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-9	Heterosigma akashiwo		Marine (Sediment)	Red tide
NIES-10	Heterosigma akashiwo		Marine (Sediment)	Red tide
NIES-6	Heterosigma akashiwo		Marine	Red tide
NIES-2857	Heterosigma akashiwo		Marine (Seawater)	Red tide
NIES-293	Heterosigma akashiwo		Marine (Seawater)	Red tide ; Genome decoded strain (Cattolico, R. A. et al. 2008)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-146	Heterosigma akashiwo		Marine	Red tide

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-590	Odontella longicruris	Centric diatom	Marine (Seawater)	Red tide
NIES-15	Olisthodiscus tomasii		Marine (Coastal soil)	Red tide ; Authentic strain, Holotype (Barcytė et al. 2021)
NIES-161	Chattonella marina		Marine	Red tide
NIES-1308	Calyptrosphaera sphaeroidea	Coccolithophore	Marine (Seawater)	Red tide ; Planktonic ; Heterococcolith stage
NIES-85	Chattonella marina		Marine	Red tide

Reference
Iwasaki, H. & Sasada, K. 1969 Studies on the red tide dinoflagellates - II. On Heterosigma inlandica appeared in Gokasho Bay, Shima Peninsula. Bull. Jpn. Soc. Sci. Fish., 35, 943-947 (in Japanese with English summary). Strain(s): 5 Sako, Y., Adachi, M., Ishida, Y. 1995 Specific monoclonal antibodies and DNA probes for the identification of the toxic dinoflagellate genus Alexandrium. In Harmful Marine Algal Blooms, Eds. by Lassus, P., et al., Lavoisier, Intercept Ltd., pp. 77-82. Strain(s): 5, 118 Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1986, 2383, 2391, 2405, 2406 DOI: 10.2216/08-98.1 Iwata, Y., Sugahara, I., Kimura, T., Kowa, H., Matsumoto, A., Noritake, K. 2004 Properties of an algicidal bacterium (Flavobacterium sp.) against Karenia mikimotoi isolated from Ise Bay, Japan. Nippon Suisan Gakkaishi, 70, 537-541 (in Japanese with English summary). Keywords: algicidal bacteria; red tide; Karenia mikimotoi; Flavobacterium Strain(s): 1, 3, 5, 32 DOI: 10.2331/suisan.70.537 Yamaguchi, H., Nishijima, T., Nishitani, H., Fukami, K., Adachi, M. 2004 Organic phosphorus utilization and alkaline phosphatase production of 3 red tide photoplankton. Nippon Suisan Gakkaishi, 70, 123-130 (in Japanese with English summary). Keywords: Karenia mikimotoi; Skeletonema costatum; Heterosigma akashiwo; red tide phytoplankton; organic phosphorus; alkaline phosphatase Strain(s): 5, 324 DOI: 10.2331/suisan.70.123 Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227. Strain(s): 5, 15, 161 DOI: 10.2216/i0031-8884-28-2-222.1 Hosoi-Tanabe, S., Otake, I., Sako, Y. 2006 Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family. Fish. Sci., 72, 1200-1208. Keywords: Chattonella; genetic homogeneity; phylogenetic analysis; Raphidophyceae; rRNA gene family Strain(s): 1, 5, 86, 121, 558, 559, 603, 670 DOI: 10.1111/j.1444-2906.2006.01277.x Yoshimura, T., Yanagawa, T., Fujiwara, A., Murai, K., Nogata, Y., Furuta, T., Kobayashi, T. 2016 Marine diatom Coscinodiscus wailesii inhibits DPD measurement of residual chlorine in seawater samples. Sessile Organisms, 33, 38-43 (in Japanese with English summary). Keywords: Residual chlorine; DPD; Seawater; Coscinodiscus wailesii Strain(s): 1, 5, 237, 323 DOI: 10.4282/sosj.33.38 Takenaka, S., Yoshikawa, T., Kadowaki, S., Okunishi, S., Maeda, H. 2017 Microflora in the soft Ttissue of the pacific oyster Crassostrea gigas exposed to the harmful microalga Heterosigma akashiwo. Biocontrol Science, 22, 79-87. Keywords: Pacific oyster; Heterosigma akashiwo; Filtraion rate; Intestinal microflora Strain(s): 5 PubMed: 28659559 DOI: 10.4265/bio.22.79 Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID). Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778 DOI: 10.1016/j.algal.2021.102191 Kim, J.-H., Park, B. S., Kim, J. H. 2023 Comprehensive understanding of the life history of harmful raphidophyte Heterosigma akashiwo: Integrating in situ and in vitro observations. Harmful Algae, 129, 102521 (article ID). Keywords: Heterosigma akashiwo; Harmful alga; Raphidophyceae; Life history; Life cycle; Sexual reproduction; Hologamete; Homothallism; Zygote (large cell); Multiple cells; Cell cluster; Cyst cluster Strain(s): 5, 293 PubMed: 37951620 DOI: 10.1016/j.hal.2023.102521 Takatsuka, S., Miyamoto, N., Sato, H., Morino, Y., Kurita, Y., Yabuki, A., Chen, C., Kawagucci, S. 2024 Millisecond-scale behaviours of plankton quantified in vitro and in situ using the Event-based Vision Sensor. Ecol. Evol., 14, e70150 (article ID). Keywords: computer vision; deep sea; dynamic vision sensor; event camera; high-speed camera; marineparticles Strain(s): 1, 5 PubMed: 39206462 DOI: 10.1002/ece3.70150

Reference

Iwasaki, H. & Sasada, K. 1969 Studies on the red tide dinoflagellates - II. On Heterosigma inlandica appeared in Gokasho Bay, Shima Peninsula. Bull. Jpn. Soc. Sci. Fish., 35, 943-947 (in Japanese with English summary).
Strain(s): 5

Sako, Y., Adachi, M., Ishida, Y. 1995 Specific monoclonal antibodies and DNA probes for the identification of the toxic dinoflagellate genus Alexandrium. In Harmful Marine Algal Blooms, Eds. by Lassus, P., et al., Lavoisier, Intercept Ltd., pp. 77-82.
Strain(s): 5, 118

Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy
Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1986, 2383, 2391, 2405, 2406
DOI: 10.2216/08-98.1

Iwata, Y., Sugahara, I., Kimura, T., Kowa, H., Matsumoto, A., Noritake, K. 2004 Properties of an algicidal bacterium (Flavobacterium sp.) against Karenia mikimotoi isolated from Ise Bay, Japan. Nippon Suisan Gakkaishi, 70, 537-541 (in Japanese with English summary).
Keywords: algicidal bacteria; red tide; Karenia mikimotoi; Flavobacterium
Strain(s): 1, 3, 5, 32
DOI: 10.2331/suisan.70.537

Yamaguchi, H., Nishijima, T., Nishitani, H., Fukami, K., Adachi, M. 2004 Organic phosphorus utilization and alkaline phosphatase production of 3 red tide photoplankton. Nippon Suisan Gakkaishi, 70, 123-130 (in Japanese with English summary).
Keywords: Karenia mikimotoi; Skeletonema costatum; Heterosigma akashiwo; red tide phytoplankton; organic phosphorus; alkaline phosphatase
Strain(s): 5, 324
DOI: 10.2331/suisan.70.123

Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227.
Strain(s): 5, 15, 161
DOI: 10.2216/i0031-8884-28-2-222.1

Hosoi-Tanabe, S., Otake, I., Sako, Y. 2006 Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family. Fish. Sci., 72, 1200-1208.
Keywords: Chattonella; genetic homogeneity; phylogenetic analysis; Raphidophyceae; rRNA gene family
Strain(s): 1, 5, 86, 121, 558, 559, 603, 670
DOI: 10.1111/j.1444-2906.2006.01277.x

Yoshimura, T., Yanagawa, T., Fujiwara, A., Murai, K., Nogata, Y., Furuta, T., Kobayashi, T. 2016 Marine diatom Coscinodiscus wailesii inhibits DPD measurement of residual chlorine in seawater samples. Sessile Organisms, 33, 38-43 (in Japanese with English summary).
Keywords: Residual chlorine; DPD; Seawater; Coscinodiscus wailesii
Strain(s): 1, 5, 237, 323
DOI: 10.4282/sosj.33.38

Takenaka, S., Yoshikawa, T., Kadowaki, S., Okunishi, S., Maeda, H. 2017 Microflora in the soft Ttissue of the pacific oyster Crassostrea gigas exposed to the harmful microalga Heterosigma akashiwo. Biocontrol Science, 22, 79-87.
Keywords: Pacific oyster; Heterosigma akashiwo; Filtraion rate; Intestinal microflora
Strain(s): 5
PubMed: 28659559
DOI: 10.4265/bio.22.79

Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID).
Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles
Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778
DOI: 10.1016/j.algal.2021.102191

Kim, J.-H., Park, B. S., Kim, J. H. 2023 Comprehensive understanding of the life history of harmful raphidophyte Heterosigma akashiwo: Integrating in situ and in vitro observations. Harmful Algae, 129, 102521 (article ID).
Keywords: Heterosigma akashiwo; Harmful alga; Raphidophyceae; Life history; Life cycle; Sexual reproduction; Hologamete; Homothallism; Zygote (large cell); Multiple cells; Cell cluster; Cyst cluster
Strain(s): 5, 293
PubMed: 37951620
DOI: 10.1016/j.hal.2023.102521

Takatsuka, S., Miyamoto, N., Sato, H., Morino, Y., Kurita, Y., Yabuki, A., Chen, C., Kawagucci, S. 2024 Millisecond-scale behaviours of plankton quantified in vitro and in situ using the Event-based Vision Sensor. Ecol. Evol., 14, e70150 (article ID).
Keywords: computer vision; deep sea; dynamic vision sensor; event camera; high-speed camera; marineparticles
Strain(s): 1, 5
PubMed: 39206462
DOI: 10.1002/ece3.70150

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