Strain Data

A big image opens when the thumbnail is clicked.(nies-1333.jpg)

Strain number		NIES-1333
Phylum		Haptophyta
Class		Prymnesiophyceae
Scientific name		Chrysochromulina sp.
Synonym
Former name
Common name
Locality (Date of collection)		Tokyo Bay Japan (2003-10-16)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< TKB;
Isolator (Date of isolation)		Okamoto, Noriko (2003-12-**)
Identified by		Okamoto, Noriko
State of strain		Subculture; Unialgal; Non-clonal; Non-axenic
Culture condition (Preculture condition)		Medium: ESM Temperature: 20 C Light intensity: 4-5 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		Mitochondrial genome ( AB930144 ) , 18S rRNA ( DQ980478 ) , 28S rRNA ( DQ980468 DQ980469 ) , actin ( DQ980417 DQ980418 DQ980438 ) , alpha-tubulin ( DQ980487 DQ980488 DQ980532 DQ980533 ) , beta-tubulin ( AB194979 DQ980520 DQ980513 DQ980514 DQ980519 ) , EFL ( AB377267 ) , Hsp90 ( ABJ80934 DQ980442 DQ980443 DQ980444 ) , rbcL ( AB510942 )
Cell size (min - max)		- 5 μm
Organization		Unicellular; Flagellate
Characteristics		Genome decoded strain (Nishimura et al. 2014) ; Prey for NIES-1334 and NIES-1335
Other strain no.		Other strain no. : TKB-079 (nrc063)
Remarks
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2506	Chrysochromulina sp.		Marine (Seawater)	Planktonic
NIES-4122	Chrysochromulina sp.		Marine (Seawater)
NIES-562	Chrysochromulina parva		Freshwater (Lake water)	Planktonic
NIES-4257	Chrysochromulina andersonii		Marine (the host radiolarian Dictyocoryne truncatum)	Planktonic ; Benthic ; Symbiotic (radiolarian) ; Authentic strain (Yuasa et al. 2019)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1392	Chrysochromulina simplex		Marine (Seawater)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1397	Prymnesium sp.		Marine (Seawater)
NIES-1013	Placidia cafeteriopsis		Marine (Seawater)	Heterotrophic ; Phagotrophic
NIES-324	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Seawater)	Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-1964	Ochrosphaera neapolitana	Coccolithophore	Marine (Seawater)	Planktonic
NIES-2584	Lotharella reticulosa		Marine (Seawater)	Planktonic

Reference
Okamoto, N. & Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179. Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA Strain(s): 1333, 1334, 1335 PubMed: 16171184 DOI: 10.1016/j.protis.2004.12.003 Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466. Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution Strain(s): 936, 955, 1333, 1335 PubMed: 16982820 DOI: 10.1093/molbev/msl120 Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID). Strain(s): 1333, 1335 PubMed: 22606358 DOI: 10.1371/journal.pone.0037307 Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2014 An intronic open reading frame was released from one of group II introns in the mitochondrial genome of the haptophyte Chrysochromulina sp. NIES-1333. Mobile Genetic Elements, 4, e29384 (article ID). Keywords: group II intron; mitochondrial genome; intron encoded protein; lateral intron transfer; Chrysochromulina sp. Strain(s): 1333 PubMed: 25054084 DOI: 10.4161/mge.29384 Edvardsen, B., Eikrem, W., Throndsen, J., Sáez, A. G., Probert, I., Medlin, L. K. 2011 Ribosomal DNA phylogenies and a morphological revision provide the basis for a revised taxonomy of the Prymnesiales (Haptophyta). Eur. J. Phycol., 46, 202-228. Keywords: Chrysochromulinaceae; Haptophyta; phylogeny; phytoplankton; Prymnesiales; Pseudohaptolina arctica; ribosomal DNA; taxonomy Strain(s): 1333, 1393 DOI: 10.1080/09670262.2011.594095 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Nishimura, Y. 2016 Diversity and evolution of protist mitochondria : introns, gene content and genome architecture. PhD thesis, University of Tsukuba, Japan, 75 pp. Strain(s): 1333, 1335 Jamy, M., Huber, T., Antoine, T., Ruscheweyh, H-J., Paoli, L., Pelletier, E., Delmont, T. O., Burki, F. 2025 Identification of a deep-branching lineage of algae using environmental plastid genomes. Nat. Commun., 17, 662 (article ID). Strain(s): 1333 PubMed: 41392160 DOI: 10.1038/s41467-025-67401-4

Reference

Okamoto, N. & Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179.
Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA
Strain(s): 1333, 1334, 1335
PubMed: 16171184
DOI: 10.1016/j.protis.2004.12.003

Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466.
Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution
Strain(s): 936, 955, 1333, 1335
PubMed: 16982820
DOI: 10.1093/molbev/msl120

Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID).
Strain(s): 1333, 1335
PubMed: 22606358
DOI: 10.1371/journal.pone.0037307

Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2014 An intronic open reading frame was released from one of group II introns in the mitochondrial genome of the haptophyte Chrysochromulina sp. NIES-1333. Mobile Genetic Elements, 4, e29384 (article ID).
Keywords: group II intron; mitochondrial genome; intron encoded protein; lateral intron transfer; Chrysochromulina sp.
Strain(s): 1333
PubMed: 25054084
DOI: 10.4161/mge.29384

Edvardsen, B., Eikrem, W., Throndsen, J., Sáez, A. G., Probert, I., Medlin, L. K. 2011 Ribosomal DNA phylogenies and a morphological revision provide the basis for a revised taxonomy of the Prymnesiales (Haptophyta). Eur. J. Phycol., 46, 202-228.
Keywords: Chrysochromulinaceae; Haptophyta; phylogeny; phytoplankton; Prymnesiales; Pseudohaptolina arctica; ribosomal DNA; taxonomy
Strain(s): 1333, 1393
DOI: 10.1080/09670262.2011.594095

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Nishimura, Y. 2016 Diversity and evolution of protist mitochondria : introns, gene content and genome architecture. PhD thesis, University of Tsukuba, Japan, 75 pp.
Strain(s): 1333, 1335

Jamy, M., Huber, T., Antoine, T., Ruscheweyh, H-J., Paoli, L., Pelletier, E., Delmont, T. O., Burki, F. 2025 Identification of a deep-branching lineage of algae using environmental plastid genomes. Nat. Commun., 17, 662 (article ID).
Strain(s): 1333
PubMed: 41392160
DOI: 10.1038/s41467-025-67401-4

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