Strain Data

A big image opens when the thumbnail is clicked.(nies-1335.jpg)

Strain number		NIES-1335
Phylum		Kathablepharida
Class		Kathablepharidea
Scientific name		Leucocryptos marina (Braarud) Butcher
Synonym
Former name
Common name
Locality (Date of collection)		Tokyo Bay Japan (2003-10-16)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< TKB;
Isolator (Date of isolation)		Okamoto, Noriko (2003-12-**)
Identified by		Okamoto, Noriko
State of strain		Subculture; Mixed (Chrysochromulina sp. NIES-1333 as food); Non-clonal; Non-axenic
Culture condition (Preculture condition)		Medium: mIMR Temperature: 20 C Light intensity: 4-5 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		Mitochondrial genome ( LC515368 ) , 18S rRNA ( AB194980 DQ980480 DQ980481 ) , LSU rRNA ( DQ980471 ) , actin ( DQ980424 DQ980425 DQ980426 DQ980439 DQ980440 DQ980441 ) , alpha-tubulin ( DQ980496 DQ980535 DQ980537 DQ980538 ) , beta-tubulin ( AB194977 AB194978 DQ980511 FJ973420 ) , eEF-2 ( EU812190 EU812191 EU812192 EU812193 EU812194 EU812195 ) , Hsp70 ( EU812178 EU812179 EU812180 EU812181 EU812182 ) , Hsp90 ( DQ980461 DQ980462 DQ980463 ) , nad11 ( AB693966 )
Cell size (min - max)		- 15 μm
Organization		Unicellular; Flagellate
Characteristics		Heterotrophic ; Phagotrophic ; Genome decoded strain (Nishimura et al. 2020)
Other strain no.		Other strain no. : TKB-081 (nrc057)
Remarks
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-997	Calyptrosphaera sphaeroidea	Coccolithophore	Marine (Seawater)	Planktonic ; Holococcolith stage
NIES-2149	Euglena viridis
NIES-8	Chrysotila roscoffensis	Coccolithophore	Marine	Red tide
NIES-2433	Norrisiella sphaerica		Marine (Seaweed)
NIES-1397	Prymnesium sp.		Marine (Seawater)

Reference
Okamoto, N. & Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179. Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA Strain(s): 1333, 1334, 1335 PubMed: 16171184 DOI: 10.1016/j.protis.2004.12.003 Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466. Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution Strain(s): 936, 955, 1333, 1335 PubMed: 16982820 DOI: 10.1093/molbev/msl120 Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID). Strain(s): 1333, 1335 PubMed: 22606358 DOI: 10.1371/journal.pone.0037307 Kim, E., Graham, L. E. 2008 EEF2 analysis challenges the monophyly of Archaeplastida and Chromalveolata. PLoS One, 3, e0002621 (article ID). Strain(s): 1335 PubMed: 18612431 DOI: 10.1371/journal.pone.0002621 Reeb, V. C., Peglar, M. T., Yoon, H. S., Bai, J. R., Wu, M., Siu, P., Grafenberg, J. L., Reyes-Prieto, A., Rummele, S. E., Gross, J., Bhattacharya, D. 2009 Interrelationships of chromalveolates within a broadly sampled tree of photosynthetic protists. Mol. Phylogenet. Evol., 53, 202–211. Keywords: Chromalveolata; Multigene phylogenetics; Plastidophila; Rhizaria; Supergroup; Tree of life Strain(s): 1334, 1335 PubMed: 19398025 DOI: 10.1016/j.ympev.2009.04.012 Nishimura. Y., Kume, K., Sonehara, K., Tanifuji, G., Shiratori, T., Ishida, K-I., Hashimoto, T., Inagaki, Y., Ohkuma, M. 2020 Mitochondrial genomes of Hemiarma marina and Leucocryptos marina revised the evolution of cytochrome c maturation in Cryptista. Front. Ecol. Evol., 8, 140 (article ID). Keywords: kathablepharids; cytochrome c maturation; goniomonads; cryptophytes; holocytochrome c synthase Strain(s): 1335 DOI: 10.3389/fevo.2020.00140 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Nishimura, Y. 2016 Diversity and evolution of protist mitochondria : introns, gene content and genome architecture. PhD thesis, University of Tsukuba, Japan, 75 pp. Strain(s): 1333, 1335

Reference

Okamoto, N. & Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179.
Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA
Strain(s): 1333, 1334, 1335
PubMed: 16171184
DOI: 10.1016/j.protis.2004.12.003

Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466.
Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution
Strain(s): 936, 955, 1333, 1335
PubMed: 16982820
DOI: 10.1093/molbev/msl120

Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID).
Strain(s): 1333, 1335
PubMed: 22606358
DOI: 10.1371/journal.pone.0037307

Kim, E., Graham, L. E. 2008 EEF2 analysis challenges the monophyly of Archaeplastida and Chromalveolata. PLoS One, 3, e0002621 (article ID).
Strain(s): 1335
PubMed: 18612431
DOI: 10.1371/journal.pone.0002621

Reeb, V. C., Peglar, M. T., Yoon, H. S., Bai, J. R., Wu, M., Siu, P., Grafenberg, J. L., Reyes-Prieto, A., Rummele, S. E., Gross, J., Bhattacharya, D. 2009 Interrelationships of chromalveolates within a broadly sampled tree of photosynthetic protists. Mol. Phylogenet. Evol., 53, 202–211.
Keywords: Chromalveolata; Multigene phylogenetics; Plastidophila; Rhizaria; Supergroup; Tree of life
Strain(s): 1334, 1335
PubMed: 19398025
DOI: 10.1016/j.ympev.2009.04.012

Nishimura. Y., Kume, K., Sonehara, K., Tanifuji, G., Shiratori, T., Ishida, K-I., Hashimoto, T., Inagaki, Y., Ohkuma, M. 2020 Mitochondrial genomes of Hemiarma marina and Leucocryptos marina revised the evolution of cytochrome c maturation in Cryptista. Front. Ecol. Evol., 8, 140 (article ID).
Keywords: kathablepharids; cytochrome c maturation; goniomonads; cryptophytes; holocytochrome c synthase
Strain(s): 1335
DOI: 10.3389/fevo.2020.00140

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Nishimura, Y. 2016 Diversity and evolution of protist mitochondria : introns, gene content and genome architecture. PhD thesis, University of Tsukuba, Japan, 75 pp.
Strain(s): 1333, 1335

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