Strain Data

Strain number		NIES-60
Phylum		Streptophyta
Class		Zygnematophyceae
Scientific name		Closterium peracerosum-strigosum-littorale complex
Synonym
Former name
Common name		Desmid
Locality (Date of collection)		Mito, Ibaraki, Japan (1975-06-**)
Latitude / Longitude		36.370525 / 140.435609
Habitat (Isolation source)		Freshwater (Paddy water)
History		< Watanabe, Makoto M.
Isolator (Date of isolation)		Watanabe, Makoto M. (1975-06-**)
Identified by		Watanabe, Makoto M.
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 15 (20) C Light intensity: 7 (10-17) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (21 D)
Gene information		18S rRNA ( AB625557 ) , 5.8S-ITS2-28S ( AB625549 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Heterothallic: Mating type (+) ; Mating group II B
Other strain no.		Other strain no. : IB-12-1
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-64	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (−) ; Mating group II B
NIES-65	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (+) ; Mating group II B
NIES-52	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Paddy water)	Heterothallic: Mating type (−) ; Mating group II A
NIES-67	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (+) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-68	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (−) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-228	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229
NIES-229	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-4550	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Vegetative cell germinated from a zygospore)	Homothallic ; Isogamou ; Genome decoded strain (Kawaguchi et al. 2023)
NIES-724	Eudorina unicocca	Green alga	Freshwater	Phototaxis ; Heterothallic
NIES-457	Eudorina elegans	Green alga	Freshwater (Moat water)	Phototaxis ; Heterothallic ; Mating type (female) ; Crosses with NIES-456
NIES-166	Closterium calosporum	Desmid		Heterothallic: Mating type (−) ; Crossing with NIES-164, 165 and 167
NIES-532	Stigeoclonium fasciculare	Green alga	Freshwater (Lake water)

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs. Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 DOI: 10.1007/BF02489099 Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9. Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666, 4581, 4582 PubMed: 21858438 DOI: 10.1007/s00497-011-0174-z Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary). Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551 Tsuchikane, Y., Watanabe, M., Kawaguchi, Y. W., Uehara, K., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2024 Diversity of genome size and chromosome number in homothallic and heterothallic strains of the Closterium peracerosum–strigosum–littorale complex (Desmidiales, Zygnematophyceae, Streptophyta). J. Phycol., 60, 654-667. Keywords: Closterium; flow cytometry; genome size; heterothallism; homothallism; sexual reproduction Strain(s): 52, 53, 54, 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 261, 2666, 4319, 4320, 4321, 4322, 4548, 4549, 4550, 4551, 4552, 4581, 4582, 4583, 4584 PubMed: 38678594 DOI: 10.1111/jpy.13457

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261
DOI: 10.1007/BF02489099

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666, 4581, 4582
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary).
Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551

Tsuchikane, Y., Watanabe, M., Kawaguchi, Y. W., Uehara, K., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2024 Diversity of genome size and chromosome number in homothallic and heterothallic strains of the Closterium peracerosum–strigosum–littorale complex (Desmidiales, Zygnematophyceae, Streptophyta). J. Phycol., 60, 654-667.
Keywords: Closterium; flow cytometry; genome size; heterothallism; homothallism; sexual reproduction
Strain(s): 52, 53, 54, 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 261, 2666, 4319, 4320, 4321, 4322, 4548, 4549, 4550, 4551, 4552, 4581, 4582, 4583, 4584
PubMed: 38678594
DOI: 10.1111/jpy.13457

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