Strain Data

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Strain number		NIES-69
Phylum		Streptophyta
Class		Zygnematophyceae
Scientific name		Closterium peracerosum-strigosum-littorale complex
Synonym
Former name
Common name		Desmid
Locality (Date of collection)		Lake Teganuma, Abiko, Chiba, Japan (1974-06-**)
Latitude / Longitude		35.8581459719 / 140.031447988
Habitat (Isolation source)		Freshwater
History		< Watanabe, Makoto M.
Isolator (Date of isolation)		Watanabe, Makoto M. (1974-06-**)
Identified by		Watanabe, Makoto M.
State of strain		Subculture; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: C Temperature: 15 (20) C Light intensity: 7 (10-17) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (21 D)
Gene information		18S rRNA ( AB363688 ) , 5.8S-ITS2-28S ( AB625546 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Heterothallic: Mating type (+) ; Mating group II B
Other strain no.		Other strain no. : TG-2-21
Remarks
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-64	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (−) ; Mating group II B
NIES-65	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (+) ; Mating group II B
NIES-52	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Paddy water)	Heterothallic: Mating type (−) ; Mating group II A
NIES-67	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (+) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-68	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (−) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-228	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229
NIES-229	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-329	Klebsormidium sp.		Freshwater (Lake water)
NIES-479	Microthamnion kützingianum	Green alga	Freshwater (River water)
NIES-649	Gonium quadratum	Green alga	Freshwater	Phototaxis ; Heterothallic ; Isogamous ; Mating type (+) ; F1 clone of NIES-652 x 653 ; Sister clone to NIES-647, 648 and 650 from one zygote
NIES-183	Closterium moniliferum	Desmid	Freshwater (Sediment)	Heterothallic: Mating type (−) ; Crossing with NIES-182
NIES-179	Closterium gracile	Desmid	Freshwater (Sediment)	Heterothallic: Mating type (+) ; Crossing with NIES-180

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs. Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 DOI: 10.1007/BF02489099 Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9. Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666, 4581, 4582 PubMed: 21858438 DOI: 10.1007/s00497-011-0174-z Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203. Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261 PubMed: 27041716 DOI: 10.1111/j.1529-8817.2008.00577.x Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda,H., Demura, T., Ito, M. 2006 Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophcean alga Closterium peracerosum-strigosum-littorale comples. Plant Physiol., 141, 271-279. Strain(s): 67, 69 PubMed: 16565296 DOI: 10.1104/pp.116.078048 Tsuchikane, Y., Sekimoto, H. 2019 The genus Closterium, a new model organism to study sexual reproduction in streptophytes. New Phytol., 221, 99-104. Keywords: charophycean; Closterium; landplants; mating-type; sexual reproduction Strain(s): 67, 69 PubMed: 29992575 DOI: 10.1111/nph.15334 Sekimoto, H., Komiya, A., Tsuyuki, N., Kawai, J., Kanda, N., Ootsuki, R., Suzuki, Y., Toyoda, A., Fujiyama, A., Kasahara, M., Abe, J., Tsuchikane, Y., Nishiyama, T. 2023 A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium. New Phytol., 237, 1636-1651. Keywords: Closterium peracerosum-strigosum-littorale complex; mating type; RWP-RK; sex determination; sexual reproduction; streptophyta; Zygnematophyceae Strain(s): 67, 69 PubMed: 36533897 DOI: 10.1111/nph.18662 Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary). Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261
DOI: 10.1007/BF02489099

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666, 4581, 4582
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261
PubMed: 27041716
DOI: 10.1111/j.1529-8817.2008.00577.x

Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda,H., Demura, T., Ito, M. 2006 Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophcean alga Closterium peracerosum-strigosum-littorale comples. Plant Physiol., 141, 271-279.
Strain(s): 67, 69
PubMed: 16565296
DOI: 10.1104/pp.116.078048

Tsuchikane, Y., Sekimoto, H. 2019 The genus Closterium, a new model organism to study sexual reproduction in streptophytes. New Phytol., 221, 99-104.
Keywords: charophycean; Closterium; landplants; mating-type; sexual reproduction
Strain(s): 67, 69
PubMed: 29992575
DOI: 10.1111/nph.15334

Sekimoto, H., Komiya, A., Tsuyuki, N., Kawai, J., Kanda, N., Ootsuki, R., Suzuki, Y., Toyoda, A., Fujiyama, A., Kasahara, M., Abe, J., Tsuchikane, Y., Nishiyama, T. 2023 A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium. New Phytol., 237, 1636-1651.
Keywords: Closterium peracerosum-strigosum-littorale complex; mating type; RWP-RK; sex determination; sexual reproduction; streptophyta; Zygnematophyceae
Strain(s): 67, 69
PubMed: 36533897
DOI: 10.1111/nph.18662

Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary).
Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551

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