Strain Data

Strain number		NIES-2098
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Calothrix sp.
Synonym
Former name		Calothrix elenkinii Kossinskaja
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Ishikawa, Masako
Isolator (Date of isolation)		Ishikawa, Masako
Identified by		Re-identified by DNA analysis at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2013 Feb]
Culture condition (Preculture condition)		Medium: MDM (agar) Temperature: 20 C Light intensity: 9-10 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M
Gene information		Whole-genome ( AP018172 ) , 16S rRNA ( LC215281 ) , Plasmid DNA ( AP018173 )
Cell size (min - max)
Organization		Filamentous
Characteristics		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
Other strain no.		Other collection strain no. : IAM M-61 Other strain no. : Ishikawa 103X (1) W-N-F
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3974	Calothrix sp.	Blue-green alga ; Cyanobacteria	Hot spring (Stream Sediment)	Genome decoded strain
NIES-4105	Calothrix sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Concrete fence)	Nitrogen fixation ; Attached ; Genome decoded strain
NIES-2099	Calothrix sp.	Blue-green alga ; Cyanobacteria		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium)
NIES-2100	Calothrix sp.	Blue-green alga ; Cyanobacteria		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
NIES-2101	Calothrix sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance ; Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain (Zhu et al. 2017)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-22	Calothrix brevissima	Blue-green alga ; Cyanobacteria	Freshwater (Soil)	Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Chromatic adaptation
NIES-267	Calothrix parasitica	Blue-green alga ; Cyanobacteria	Marine (Seaweed)	Endophyte in Nemalion (Rhodophyceae) ; Genome decoded strain

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-846	Tychonema bourrellyi	Blue-green alga ; Cyanobacteria	Freshwater	Cyanobacterial water bloom (aoko) ; Planktonic ; Type strain
NIES-2128	Phormidium sp.	Blue-green alga ; Cyanobacteria
NIES-3277	Synechococcus leopoliensis	Blue-green alga ; Cyanobacteria		Genome decoded strain
NIES-4073	Scytonema sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Nostoc commune)	Drought tolerance ; Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium) ; Genome decoded strain
NIES-2132	Symploca muscorum	Blue-green alga ; Cyanobacteria

Reference
Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermidine Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277 MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451. Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106 PubMed: 31899489 DOI: 10.1093/molbev/msz308 Gao, J.-J., Zhang, L.. Peng, R.-H., Wang, B., Feng, H.-J., Li, Z.-J., Yao, Q.-H. 2021 Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate. Biotechnol. Biotechnol. Equip., 34, 494-505. Keywords: Thiocyanate; Thermosynechococcus elongatus BP-1; Arabidopsis thaliana; transgenic; enhance phytoremediation; glutathione S-transferase; degradation Strain(s): 2098, 2134, 4103 DOI: 10.1080/13102818.2020.1779127 Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID). Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106 PubMed: 34677568 DOI: 10.1093/dnares/dsab024 Carrell, A. A., Lawrence, T. J., Cabugao, K. G. M., Carper, D. L., Pelletier, D. A., Lee, J. H., Jawdy, S., Grimwood, J., Schmutz, J., Hanson, P. J., Shaw, A. J., Weston, D. J. 2022 Habitat-adapted microbial communities mediate Sphagnum peatmoss resilience to warming. New Phytol., 234, 2111-2125. Keywords: climate change; heat tolerance; microbiome transfer; moss; peatland; Sphagnum; symbiosis; synthetic communities Strain(s): 25, 81, 2098, 2100, 2107 DOI: 10.1111/nph.18072 Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID). Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106 PubMed: 38711016 DOI: 10.1186/s12862-024-02244-z Giannakopoulos, C., Panou, M., Gkelis, S. 2024 Phylogenetic analysis of Nostocales (Cyanobacteria) based on two novel molecular markers, implicated in the nitrogenase biosynthesis. FEMS Microbiol. Lett.,>, 371, fnad136 (article ID). Keywords: nitrogenase evolution; phylogeny; taxonomy; novel markers; nif genes; ecophysiological characters Strain(s): 21, 23, 25, 2098, 2111, 3756, 4074, 4103, 4106 PubMed: 38168702 DOI: 10.1093/femsle/fnad136

Reference

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277

MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451.
Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106
PubMed: 31899489
DOI: 10.1093/molbev/msz308

Gao, J.-J., Zhang, L.. Peng, R.-H., Wang, B., Feng, H.-J., Li, Z.-J., Yao, Q.-H. 2021 Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate. Biotechnol. Biotechnol. Equip., 34, 494-505.
Keywords: Thiocyanate; Thermosynechococcus elongatus BP-1; Arabidopsis thaliana; transgenic; enhance phytoremediation; glutathione S-transferase; degradation
Strain(s): 2098, 2134, 4103
DOI: 10.1080/13102818.2020.1779127

Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID).
Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy
Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106
PubMed: 34677568
DOI: 10.1093/dnares/dsab024

Carrell, A. A., Lawrence, T. J., Cabugao, K. G. M., Carper, D. L., Pelletier, D. A., Lee, J. H., Jawdy, S., Grimwood, J., Schmutz, J., Hanson, P. J., Shaw, A. J., Weston, D. J. 2022 Habitat-adapted microbial communities mediate Sphagnum peatmoss resilience to warming. New Phytol., 234, 2111-2125.
Keywords: climate change; heat tolerance; microbiome transfer; moss; peatland; Sphagnum; symbiosis; synthetic communities
Strain(s): 25, 81, 2098, 2100, 2107
DOI: 10.1111/nph.18072

Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID).
Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA
Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106
PubMed: 38711016
DOI: 10.1186/s12862-024-02244-z

Giannakopoulos, C., Panou, M., Gkelis, S. 2024 Phylogenetic analysis of Nostocales (Cyanobacteria) based on two novel molecular markers, implicated in the nitrogenase biosynthesis. FEMS Microbiol. Lett.,>, 371, fnad136 (article ID).
Keywords: nitrogenase evolution; phylogeny; taxonomy; novel markers; nif genes; ecophysiological characters
Strain(s): 21, 23, 25, 2098, 2111, 3756, 4074, 4103, 4106
PubMed: 38168702
DOI: 10.1093/femsle/fnad136

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.