Strain Data

Strain number		NIES-2100
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Calothrix sp.
Synonym
Former name		Calothrix gracilis Fritsch
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Ishikawa, Masako
Isolator (Date of isolation)		Ishikawa, Masako
Identified by		Re-identified by DNA analysis at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2013 Feb]
Culture condition (Preculture condition)		Medium: MDM (agar) Temperature: 20 C Light intensity: 9-10 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M
Gene information		Whole-genome ( AP018179 ) , nearly complete genome ( AP018178 ) , 16S rRNA ( LC215282 )
Cell size (min - max)
Organization		Filamentous
Characteristics		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
Other strain no.		Other collection strain no. : IAM M-56 Other strain no. : Ishikawa 78. F243D
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3974	Calothrix sp.	Blue-green alga ; Cyanobacteria	Hot spring (Stream Sediment)	Genome decoded strain
NIES-4105	Calothrix sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Concrete fence)	Nitrogen fixation ; Attached ; Genome decoded strain
NIES-2098	Calothrix sp.	Blue-green alga ; Cyanobacteria		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
NIES-2099	Calothrix sp.	Blue-green alga ; Cyanobacteria		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium)
NIES-2101	Calothrix sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance ; Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain (Zhu et al. 2017)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-22	Calothrix brevissima	Blue-green alga ; Cyanobacteria	Freshwater (Soil)	Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Chromatic adaptation
NIES-267	Calothrix parasitica	Blue-green alga ; Cyanobacteria	Marine (Seaweed)	Endophyte in Nemalion (Rhodophyceae) ; Genome decoded strain

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-80	Dolichospermum hangangense	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Planktonic ; Genome decoded strain
NIES-45	Planktothrix spiroides	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko)
NIES-2119	Phormidium ambiguum	Blue-green alga ; Cyanobacteria		Offensive odor ; geosmin production (Iwase et al. 2010) ; Hydrogen evolution ; Attached ; Genome decoded strain (Zhu et al. 2017)
NIES-847	Limnothrix redekei	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Planktonic
NIES-21	Nostoc sp.	Blue-green alga ; Cyanobacteria		Genome decoded strain

Reference
Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermidine Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277 MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451. Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106 PubMed: 31899489 DOI: 10.1093/molbev/msz308 Gharaie Fathabad, S., Arumanayagam, A. S., Tabatabai, B., Chen, H., Lu, J., Sitther, V. 2019 Augmenting Fremyella diplosiphon cellular lipid content and unsaturated fatty acid methyl esters via sterol desaturase gene overexpression. Appl. Biochem. Biotechnol., 189, 1127-1140. Keywords: Alkane; Biofuel; Cyanobacteria; Sterol desaturase; Transcript abundance Strain(s): 2100, 2107, 3585 PubMed: 31168708 DOI: 10.1007/s12010-019-03055-5 Churro, C., Semedo-Aguiar, A. P., Silva, A.D., Pereira-Leal, J. B., Leite, R. B. 2020 A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria. Sci Rep, 10, 8679 (article ID). Strain(s): 25, 2100, 2111 PubMed: 32457360 DOI: 10.1038/s41598-020-64774-y Baqué, M., Napoli, A., Fagliarone, C., Moeller, R., de Vera, J. P., Billi, D. 2020 Carotenoid raman signatures are better preserved in dried cells of the desert cyanobacterium Chroococcidiopsis than in hydrated counterparts after high-dose gamma irradiation. Life-Basel, 10, 83 (article ID). Keywords: biosignatures; extremophiles; ionizing radiation; Raman spectroscopy; cyanobacteria; Mars exploration Strain(s): 25, 2100, 4073, 4103, 4105 PubMed: 32521820 DOI: 10.3390/life10060083 Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID). Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106 PubMed: 34677568 DOI: 10.1093/dnares/dsab024 Carrell, A. A., Lawrence, T. J., Cabugao, K. G. M., Carper, D. L., Pelletier, D. A., Lee, J. H., Jawdy, S., Grimwood, J., Schmutz, J., Hanson, P. J., Shaw, A. J., Weston, D. J. 2022 Habitat-adapted microbial communities mediate Sphagnum peatmoss resilience to warming. New Phytol., 234, 2111-2125. Keywords: climate change; heat tolerance; microbiome transfer; moss; peatland; Sphagnum; symbiosis; synthetic communities Strain(s): 25, 81, 2098, 2100, 2107 DOI: 10.1111/nph.18072 Arsın, S., Delbaje, E., Jokela, J., Wahlsten, M., Farrar, Z. M., Permi, P., Fewer, D. 2023 A plastic biosynthetic pathway for the production of structurally distinct microbial sunscreens. ACS Chem. Biol., 18, 1959–1967. Strain(s): 25, 73, 267, 2100, 2107, 2109, 2111, 2130, 3755, 3756, 3757, 3974, 4073, 4102, 4103, 4105, 4106 PubMed: 37603862 DOI: 10.1021/acschembio.3c00112 Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID). Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106 PubMed: 38711016 DOI: 10.1186/s12862-024-02244-z

Reference

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277

MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451.
Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106
PubMed: 31899489
DOI: 10.1093/molbev/msz308

Gharaie Fathabad, S., Arumanayagam, A. S., Tabatabai, B., Chen, H., Lu, J., Sitther, V. 2019 Augmenting Fremyella diplosiphon cellular lipid content and unsaturated fatty acid methyl esters via sterol desaturase gene overexpression. Appl. Biochem. Biotechnol., 189, 1127-1140.
Keywords: Alkane; Biofuel; Cyanobacteria; Sterol desaturase; Transcript abundance
Strain(s): 2100, 2107, 3585
PubMed: 31168708
DOI: 10.1007/s12010-019-03055-5

Churro, C., Semedo-Aguiar, A. P., Silva, A.D., Pereira-Leal, J. B., Leite, R. B. 2020 A novel cyanobacterial geosmin producer, revising GeoA distribution and dispersion patterns in Bacteria. Sci Rep, 10, 8679 (article ID).
Strain(s): 25, 2100, 2111
PubMed: 32457360
DOI: 10.1038/s41598-020-64774-y

Baqué, M., Napoli, A., Fagliarone, C., Moeller, R., de Vera, J. P., Billi, D. 2020 Carotenoid raman signatures are better preserved in dried cells of the desert cyanobacterium Chroococcidiopsis than in hydrated counterparts after high-dose gamma irradiation. Life-Basel, 10, 83 (article ID).
Keywords: biosignatures; extremophiles; ionizing radiation; Raman spectroscopy; cyanobacteria; Mars exploration
Strain(s): 25, 2100, 4073, 4103, 4105
PubMed: 32521820
DOI: 10.3390/life10060083

Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID).
Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy
Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106
PubMed: 34677568
DOI: 10.1093/dnares/dsab024

Carrell, A. A., Lawrence, T. J., Cabugao, K. G. M., Carper, D. L., Pelletier, D. A., Lee, J. H., Jawdy, S., Grimwood, J., Schmutz, J., Hanson, P. J., Shaw, A. J., Weston, D. J. 2022 Habitat-adapted microbial communities mediate Sphagnum peatmoss resilience to warming. New Phytol., 234, 2111-2125.
Keywords: climate change; heat tolerance; microbiome transfer; moss; peatland; Sphagnum; symbiosis; synthetic communities
Strain(s): 25, 81, 2098, 2100, 2107
DOI: 10.1111/nph.18072

Arsın, S., Delbaje, E., Jokela, J., Wahlsten, M., Farrar, Z. M., Permi, P., Fewer, D. 2023 A plastic biosynthetic pathway for the production of structurally distinct microbial sunscreens. ACS Chem. Biol., 18, 1959–1967.
Strain(s): 25, 73, 267, 2100, 2107, 2109, 2111, 2130, 3755, 3756, 3757, 3974, 4073, 4102, 4103, 4105, 4106
PubMed: 37603862
DOI: 10.1021/acschembio.3c00112

Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID).
Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA
Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106
PubMed: 38711016
DOI: 10.1186/s12862-024-02244-z

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