Strain Data

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Strain number		NIES-50
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Aulosira laxa Kirchner ex Bornet & Flauhault
Synonym
Former name		Aulosira fertilissima Ghose (in IAM)
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Pusa, India
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Paddy soil)
History		< IAM (1983);
Isolator (Date of isolation)		Venkataraman, G. S.
Identified by
State of strain		Cryopreservation; Unialgal; Non-clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: MDM (agar) Temperature: 20 (25) C Light intensity: 4-10 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M (14 D)
Gene information		nearly complete genome ( AP018307 ) , 16S-ITS-23S ( KJ920354 KJ920353 ) , Plasmid DNA ( AP018308-AP018313 )
Cell size (min - max)		- 6 μm
Organization
Characteristics		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
Other strain no.		Other collection strain no. : IAM M-128
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-172	Closterium moniliferum	Desmid		Homothallic
NIES-504	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Freshwater (River sediment)
NIES-479	Microthamnion kützingianum	Green alga	Freshwater (River water)
NIES-34	Phormidium foveolarum	Blue-green alga ; Cyanobacteria
NIES-448	Closterium acerosum	Desmid	Freshwater

Reference
Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579 Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermidine Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277 Suradkar, A., Villanueva, C., Gaysina, LA., Casamatta, DA., Saraf, A., Dighe, G., Mergu, R., Singh, P. 2017 Nostoc thermotolerans sp. nov., a soil-dwelling species of Nostoc (Cyanobacteria). Int. J. Syst. Evol. Microbiol., 67, 1296-1305. Keywords: cyanobacteria; Taxonomy; Nostoc Strain(s): 21, 40, 50, 3756 PubMed: 28109209 DOI: 10.1099/ijsem.0.001800 Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID). Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106 PubMed: 34677568 DOI: 10.1093/dnares/dsab024 Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611. Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074 PubMed: 35595516 DOI: 10.1093/jxb/erac219 Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID). Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106 PubMed: 38711016 DOI: 10.1186/s12862-024-02244-z Pham, H. T. L., Ngo, T. T., Tran, T. V., Duong, T. A., Tran, L. D., Tran, A. T. T., Nguyen, V. T. H., Nguyen, S. V. 2025 Classification of Nostoc-like cyanobacteria isolated from paddy soil into Aliinostoc, Aulosira, and Desmonostoc. Front. Microbiol., 16, 1581725 (article ID). Keywords: Aliinostoc; Aulosira; Desmonostoc; cyanobacteria classification; Nostoc-like morphology; paddy soil; polyphasic approach Strain(s): 50 PubMed: 40463442 DOI: 10.3389/fmicb.2025.1581725

Reference

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277

Suradkar, A., Villanueva, C., Gaysina, LA., Casamatta, DA., Saraf, A., Dighe, G., Mergu, R., Singh, P. 2017 Nostoc thermotolerans sp. nov., a soil-dwelling species of Nostoc (Cyanobacteria). Int. J. Syst. Evol. Microbiol., 67, 1296-1305.
Keywords: cyanobacteria; Taxonomy; Nostoc
Strain(s): 21, 40, 50, 3756
PubMed: 28109209
DOI: 10.1099/ijsem.0.001800

Hirose, Y., Ohtsubo, Y., Misawa, N., Yonekawa, C., Nagao, N., Shimura, Y., Fujisawa, T., Kanesaki, Y., Katoh, H., Katayama, M., Yamaguchi, H., Yoshikawa, H., Ikeuchi, M., Eki, T., Nakamura, Y., Kawachi, M. 2021 Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade. DNA Res., 28, dsab024 (article ID).
Keywords: Cyanobacteria; genome; heterocyst; culture collection; taxonomy
Strain(s): 19, 21, 22, 23, 25, 37, 50, 73, 267, 806, 932, 2098, 2100, 2107, 2109, 2111, 2130, 2135, 3275, 3585, 3757, 3974, 4070, 4073, 4074, 4075, 4101, 4102, 4103, 4106
PubMed: 34677568
DOI: 10.1093/dnares/dsab024

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Nowruzi, B., Hutarova, L., Vešelenyiova, D., Metcalf, J. S. 2024 Characterization of Neowestiellopsis persica A1387 (Hapalosiphonaceae) based on the cpcA, psbA, rpoC1, nifH and nifD gene sequences. BMC Ecol. Evol., 24, 57 (article ID).
Keywords: Cyanobacteria; Phylogenetic analysis; Neowestiellopsis persica, cpcA
Strain(s): 21, 22, 23, 25, 50, 73, 267, 592, 2098, 2100, 2107, 3275, 3754, 3756, 3974, 4073, 4074, 4101, 4103, 4106
PubMed: 38711016
DOI: 10.1186/s12862-024-02244-z

Pham, H. T. L., Ngo, T. T., Tran, T. V., Duong, T. A., Tran, L. D., Tran, A. T. T., Nguyen, V. T. H., Nguyen, S. V. 2025 Classification of Nostoc-like cyanobacteria isolated from paddy soil into Aliinostoc, Aulosira, and Desmonostoc. Front. Microbiol., 16, 1581725 (article ID).
Keywords: Aliinostoc; Aulosira; Desmonostoc; cyanobacteria classification; Nostoc-like morphology; paddy soil; polyphasic approach
Strain(s): 50
PubMed: 40463442
DOI: 10.3389/fmicb.2025.1581725

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