Strain Data

Strain number		NIES-338
Phylum		Streptophyta
Class		Zygnematophyceae
Scientific name		Closterium rostratum Ehrenberg ex Ralfs var. subrostratum (Krieger) Krieger
Synonym		Closterium subrostratum Krieger
Former name
Common name		Desmid
Locality (Date of collection)		Kathmandu, Nepal (1968-05-18)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Sediment)
History		< IAM (1983);
Isolator (Date of isolation)		Ichimura, Terunobu
Identified by		Ichimura, Terunobu
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: CA Temperature: 20 (20) C Light intensity: 7-8 (13-21) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (1 M)
Gene information
Cell size (min - max)
Organization		Unicellular
Characteristics		Homothallic
Other strain no.		Other collection strain no. : IAM C-446 (=C-579) Other strain no. : N-90-55
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-228	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229
NIES-229	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228
NIES-67	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (+) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-68	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (−) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-172	Closterium moniliferum	Desmid		Homothallic

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-65	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (+) ; Mating group II B

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-459	Eudorina illinoisensis	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Mating type (female) ; Crosses with NIES-460
NIES-29	Nostoc minutum	Blue-green alga ; Cyanobacteria
NIES-464	Gloeomonas lateperforata	Green alga	Freshwater (Pond water)
NIES-724	Eudorina unicocca	Green alga	Freshwater	Phototaxis ; Heterothallic
NIES-540	Uronema gigas	Green alga	Freshwater (River water)

Reference
Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs. Strain(s): 66, 67, 68, 125, 127, 174, 175, 176, 177, 179, 180, 181, 182, 183, 185, 198, 202, 337, 338, 450, 451 Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese). Strain(s): 66, 173, 175, 179, 180, 181, 183, 198, 338, 450

Reference

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 66, 67, 68, 125, 127, 174, 175, 176, 177, 179, 180, 181, 182, 183, 185, 198, 202, 337, 338, 450, 451

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese).
Strain(s): 66, 173, 175, 179, 180, 181, 183, 198, 338, 450

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