Strain Data

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Strain number		NIES-2134
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Thermosynechococcus vulcanus (J.J.Copeland) Katoh, Itoh, Shen & Ikeuchi nom. nud.
Synonym		Synechococcus vulcanus
Former name
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Yunomine Hot Spring, Wakayama, Japan
Latitude / Longitude
Habitat (Isolation source)		Hot spring
History		< IAM (2007);
Isolator (Date of isolation)
Identified by
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2014 Mar]
Culture condition (Preculture condition)		Medium: BG-11 Temperature: 50 C Light intensity: 50-70 µmol photons/m²/sec, L/D cycle: 12L:12D Duration: 21 D
Gene information		Whole-genome ( AP018202 ) , 16S rRNA ( LC319784 MF191712 )
Cell size (min - max)
Organization
Characteristics		Thermophilic (opt. 57℃（) ; Genome decoded strain
Other strain no.		Other collection strain no. : IAM M-293
Remarks		Cryopreserved; Fragile species to temperature changes; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2133	Thermosynechococcus elongatus	Blue-green alga ; Cyanobacteria	Hot spring	Thermophilic (opt. 57℃（) ; Genome decoded strain

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2098	Calothrix sp.	Blue-green alga ; Cyanobacteria		Nitrogen fixation　 (aerobic condition, Modified BG-11₀ medium, N-Free medium) ; Genome decoded strain
NIES-970	Synechococcus sp.	Blue-green alga ; Cyanobacteria ; Picoplankton	Marine (Tidal flat sand water)	Picoplankton ; Genome decoded strain (Shimura et al. 2017)
NIES-2107	Nostoc carneum	Blue-green alga ; Cyanobacteria		Chromatic adaptation ; Genome decoded strain
NIES-806	Dolichospermum compactum	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Genome decoded strain
NIES-3373	Spirulina subsalsa	Blue-green alga ; Cyanobacteria	Marine (Tidal flat sediment)	Thermophilic ; Halophilic ; Attached ; Type strain (Kuroiwa et al. 2014)

Reference
Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2016 Additional analysis of cyanobacterial polyamines ─ Distributions of spermidine, homospermidine, spermine, and thermospermine within the phylum Cyanobacteria. ─ Microb. Resour. Syst., 32, 179-186. Keywords: cyanobacteria; homospermidine; polyamine; thermospermine Strain(s): 2086, 2087, 2134, 2412, 2539, 3373 DOI: 10.60369/microresys.32.2_179 Lianga, Y., Kaczmarek, M. B., Kasprzak, A. K., Tang, J., Shah, M. M. R., Jin, P., Klepacz-Smółk, A., Cheng, J. J., Ledakowicz, S., Daroch, M. 2018 Thermosynechococcaceae as a source of thermostable C-phycocyanins: properties and molecular insights. Algal Res., 35, 223-235. Keywords: Cyanobacteria; Phycocyanin; Thermophile; Thermostability; Phycobilisome; Natural pigment Strain(s): 2133, 2134 DOI: 10.1016/j.algal.2018.08.037 Soulier, N. & Bryant, D. A. 2020 The structural basis of far-red light absorbance by allophycocyanins. Photosynth. Res., 147, 11–26. Keywords: Far-red light photoacclimation; Low-light photoacclimation; Photosynthesis; Phycobiliproteins; Phycobilisomes; Phycocyanobilin; Site-directed mutagenesis Strain(s): 46, 2134 PubMed: 33058014 DOI: 10.1007/s11120-020-00787-y Zha, J., Steglich, C., Scholz, I., Hess, W. R., Kirilovsky, D. 2021 Inverse regulation of light harvesting and photoprotection is mediated by a 3' end-derived sRNA in cyanobacteria. Plant Cell, 33, 358-380. Strain(s): 73, 806, 2134 PubMed: 33793852 DOI: 10.1093/plcell/koaa030 Gao, J.-J., Zhang, L.. Peng, R.-H., Wang, B., Feng, H.-J., Li, Z.-J., Yao, Q.-H. 2021 Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate. Biotechnol. Biotechnol. Equip., 34, 494-505. Keywords: Thiocyanate; Thermosynechococcus elongatus BP-1; Arabidopsis thaliana; transgenic; enhance phytoremediation; glutathione S-transferase; degradation Strain(s): 2098, 2134, 4103 DOI: 10.1080/13102818.2020.1779127 Cheng, Y.-I., Chou, L., Chiu, Y.-F., Hsueh, H.-T., Kuo, C.-H., Chu, H.-A. 2020 Comparative genomic analysis of a novel strain of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. CL-1. Front. Microbiol., 11, 82 (article ID). Keywords: cyanobacterium; Thermosynechococcus; genome; comparative genomics; thermophilic; cyanobacterium; adaption Strain(s): 2134 PubMed: 32082292 DOI: 10.3389/fmicb.2020.000 Kato, K., Hamaguchi, T., Nagao, R., Kawakami, K., Ueno, Y., Suzuki, T., Uchida, H., Murakami, A., Nakajima, Y., Yokono, M., Akimoto, S., Dohmae, N., Yonekura, K., Shen, J.-R. 2022 Structural basis for the absence of low-energy chlorophylls in a photosystem I trimer from Gloeobacter violaceus. eLife, 11, e73990 (article ID). Strain(s): 2134 PubMed: 35404232 DOI: 10.7554/eLife.73990 Nakane, D., Enomoto, G., Bäehre, H., Hirose, Y., Wilde, A., Nishizaka, T. 2022 Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution. eLife, 11, e73405 (article ID). Strain(s): 2134 PubMed: 35535498 DOI: 10.7554/eLife.73405 Kawakami, K., Hamaguchi, T., Hirose, Y., Kosumi, D., Miyata, M., Kamiya, N., Yonekura, K. 2022 Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome. Nat. Commun., 13, 3389 (article ID). Strain(s): 2134 PubMed: 35715389 DOI: 10.1038/s41467-022-30962-9 Kawakami, K., Nagao, R., Tahara, Y. O., Hamaguchi, T., Suzuki, T., Dohmae, N., Kosumi, D., Shen, J.-R., Miyata, M., Yonekura, K., Kamiya, N. 2021 Structural implications for a phycobilisome complex from the thermophilic cyanobacterium Thermosynechococcus vulcanus. Biochim. Biophys. Acta-Bioenerg., 1862, 148458 (article ID). Keywords: Cyanobacteria; Electron microscopy; GraFix; Phycobilisome Strain(s): 2134 PubMed: 34062150 DOI: 10.1016/j.bbabio.2021.148458 Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611. Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074 PubMed: 35595516 DOI: 10.1093/jxb/erac219 Xiao, C. Guo, N., Liang, Z., Huang, Z., Li, W., Xie, M., Zhao, F. 2023 Ultrafast energy transfer dynamics in a cyanobacterial light-harvesting phycobilisome. Processes, 11, 1656 (article ID). Keywords: light-harvesting antenna; phycobilisomes; time-resolved spectroscopy; energy transfer Strain(s): 2134 DOI: 10.3390/pr11061656

Reference

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2016 Additional analysis of cyanobacterial polyamines ─ Distributions of spermidine, homospermidine, spermine, and thermospermine within the phylum Cyanobacteria. ─ Microb. Resour. Syst., 32, 179-186.
Keywords: cyanobacteria; homospermidine; polyamine; thermospermine
Strain(s): 2086, 2087, 2134, 2412, 2539, 3373
DOI: 10.60369/microresys.32.2_179

Lianga, Y., Kaczmarek, M. B., Kasprzak, A. K., Tang, J., Shah, M. M. R., Jin, P., Klepacz-Smółk, A., Cheng, J. J., Ledakowicz, S., Daroch, M. 2018 Thermosynechococcaceae as a source of thermostable C-phycocyanins: properties and molecular insights. Algal Res., 35, 223-235.
Keywords: Cyanobacteria; Phycocyanin; Thermophile; Thermostability; Phycobilisome; Natural pigment
Strain(s): 2133, 2134
DOI: 10.1016/j.algal.2018.08.037

Soulier, N. & Bryant, D. A. 2020 The structural basis of far-red light absorbance by allophycocyanins. Photosynth. Res., 147, 11–26.
Keywords: Far-red light photoacclimation; Low-light photoacclimation; Photosynthesis; Phycobiliproteins; Phycobilisomes; Phycocyanobilin; Site-directed mutagenesis
Strain(s): 46, 2134
PubMed: 33058014
DOI: 10.1007/s11120-020-00787-y

Zha, J., Steglich, C., Scholz, I., Hess, W. R., Kirilovsky, D. 2021 Inverse regulation of light harvesting and photoprotection is mediated by a 3' end-derived sRNA in cyanobacteria. Plant Cell, 33, 358-380.
Strain(s): 73, 806, 2134
PubMed: 33793852
DOI: 10.1093/plcell/koaa030

Gao, J.-J., Zhang, L.. Peng, R.-H., Wang, B., Feng, H.-J., Li, Z.-J., Yao, Q.-H. 2021 Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate. Biotechnol. Biotechnol. Equip., 34, 494-505.
Keywords: Thiocyanate; Thermosynechococcus elongatus BP-1; Arabidopsis thaliana; transgenic; enhance phytoremediation; glutathione S-transferase; degradation
Strain(s): 2098, 2134, 4103
DOI: 10.1080/13102818.2020.1779127

Cheng, Y.-I., Chou, L., Chiu, Y.-F., Hsueh, H.-T., Kuo, C.-H., Chu, H.-A. 2020 Comparative genomic analysis of a novel strain of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. CL-1. Front. Microbiol., 11, 82 (article ID).
Keywords: cyanobacterium; Thermosynechococcus; genome; comparative genomics; thermophilic; cyanobacterium; adaption
Strain(s): 2134
PubMed: 32082292
DOI: 10.3389/fmicb.2020.000

Kato, K., Hamaguchi, T., Nagao, R., Kawakami, K., Ueno, Y., Suzuki, T., Uchida, H., Murakami, A., Nakajima, Y., Yokono, M., Akimoto, S., Dohmae, N., Yonekura, K., Shen, J.-R. 2022 Structural basis for the absence of low-energy chlorophylls in a photosystem I trimer from Gloeobacter violaceus. eLife, 11, e73990 (article ID).
Strain(s): 2134
PubMed: 35404232
DOI: 10.7554/eLife.73990

Nakane, D., Enomoto, G., Bäehre, H., Hirose, Y., Wilde, A., Nishizaka, T. 2022 Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution. eLife, 11, e73405 (article ID).
Strain(s): 2134
PubMed: 35535498
DOI: 10.7554/eLife.73405

Kawakami, K., Hamaguchi, T., Hirose, Y., Kosumi, D., Miyata, M., Kamiya, N., Yonekura, K. 2022 Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome. Nat. Commun., 13, 3389 (article ID).
Strain(s): 2134
PubMed: 35715389
DOI: 10.1038/s41467-022-30962-9

Kawakami, K., Nagao, R., Tahara, Y. O., Hamaguchi, T., Suzuki, T., Dohmae, N., Kosumi, D., Shen, J.-R., Miyata, M., Yonekura, K., Kamiya, N. 2021 Structural implications for a phycobilisome complex from the thermophilic cyanobacterium Thermosynechococcus vulcanus. Biochim. Biophys. Acta-Bioenerg., 1862, 148458 (article ID).
Keywords: Cyanobacteria; Electron microscopy; GraFix; Phycobilisome
Strain(s): 2134
PubMed: 34062150
DOI: 10.1016/j.bbabio.2021.148458

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Xiao, C. Guo, N., Liang, Z., Huang, Z., Li, W., Xie, M., Zhao, F. 2023 Ultrafast energy transfer dynamics in a cyanobacterial light-harvesting phycobilisome. Processes, 11, 1656 (article ID).
Keywords: light-harvesting antenna; phycobilisomes; time-resolved spectroscopy; energy transfer
Strain(s): 2134
DOI: 10.3390/pr11061656

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