Strain Data

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Strain number		NIES-2142
Phylum		Heterokontophyta
Class		Chrysophyceae
Scientific name		Ochromonas danica Pringsheim
Synonym
Former name
Common name
Locality (Date of collection)		near Everdrup, Denmark
Latitude / Longitude		55.203341 / 11.94647
Habitat (Isolation source)		Freshwater
History		< IAM (2007) < UTEX (1996);
Isolator (Date of isolation)		Pringsheim, E. G. (1954--)
Identified by		Pringsheim, E. G.
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Mar]
Culture condition (Preculture condition)		Medium: O (semi-solid) Temperature: 20 C Light intensity: 13-16 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		ITS1-5.8S-ITS2 ( Y07976 ) , 16S rRNA ( X53183 ) , 28S rRNA ( KM590767 GU935636 Y07977 ) , gapC ( AJ512345 ) , gapC1 ( AJ512344 ) , psaA ( KM590836 ) , rbcL ( GU935657 ) , vppa ( AJ251725 ) , odbgp ( LC140879 )
Cell size (min - max)
Organization
Characteristics		Mixotrophic ; Phagotrophic
Other strain no.		Other collection strain no. : IAM CS-4 (=CS-2); ATCC 30004; CCAP 933/2; SAG 933-7; UTEX L 1298; CCAP 933/2B; CCMP585
Remarks		Axenic
Movie		https://www.youtube.com/watch?v=bEN7IN47VNA&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=23

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2143	Ochromonas minuta		Freshwater (Pond water)	Mixotrophic ; Phagotrophic
NIES-1828	Ochromonas sp.		Marine (Seawater)	Mixotrophic ; Phagotrophic
NIES-2300	Ochromonas sp.		Freshwater (Pond sediment)	Mixotrophic ; Phagotrophic

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2149	Euglena viridis
NIES-1401	Pavlova sp.		Marine (Seawater)	Benthic
NIES-388	Phaeocystis globosa		Marine (Seawater)	Red tide
NIES-1335	Leucocryptos marina		Marine (Seawater)	Heterotrophic ; Phagotrophic ; Genome decoded strain (Nishimura et al. 2020)
NIES-707	Chroomonas nordstedtii		Freshwater (Pond water)

Reference
McKay, R. M. & Gibbs, S. P. 1989 Immunocytochemical localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-limited and light-saturated cells of Chlorella pyrenoidosa. Protoplasma, 149, 31-37. Keywords: pyrenoid; ribulose 1,5-bisphosphate carboxylase/oxygenase; Chlorella pyrenoidosa; immunocytochemistry Strain(s): 687, 2142 Witt, D. & Stackebrandt, E. 1988 Disproving the hypothesis of a common ancestry for the Ochromonas danica chrysoplast and Heliobacterium chlorum. Arch. Mikrobiol., 150, 244-248. Strain(s): 2142 DOI: 10.1007/BF00407787 Coleman, A. W. 1988 The autofluorescent flagellum: a new phylogenetic enigma. J. Phycol., 24, 118-120. Keywords: autofluorescence; Chrysophyceae; flagellum; fluorescence; heterokont; Ochromonas; photoreceptor; Prymnesium Strain(s): 2142, 2144 DOI: 10.1111/j.1529-8817.1988.tb04465.x Kawahara, T., Kumaki, Y., Kamada, T., Ishii, T., Okino, T. 2009 Absolute configuration of chlorosulfolipids from the chrysophyta Ochromonas danica. J. Org. Chem., 74, 6016-6024. Strain(s): 2142 PubMed: 19627129 DOI: 10.1021/jo900860e Yamamoto, Y., Kawashima, D., Hashizume, A., Hisamotsu, M., Isono, N. 2013 Purification and characterization of 1,3-β-D-glucan phosphorylase from Ochromonas danica. Biosci. Biotechnol. Biochem., 77, 130411-130416. Keywords: 1,3-β-D-glucan phosphorylase; Ochromonas danica; laminarin; laminarioligosaccharides; 1,3-β-D-glucan Strain(s): 2142 PubMed: 24018693 DOI: 10.1271/bbb.130411 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 DOI: 10.60369/microresys.33.1_19 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0

Reference

McKay, R. M. & Gibbs, S. P. 1989 Immunocytochemical localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-limited and light-saturated cells of Chlorella pyrenoidosa. Protoplasma, 149, 31-37.
Keywords: pyrenoid; ribulose 1,5-bisphosphate carboxylase/oxygenase; Chlorella pyrenoidosa; immunocytochemistry
Strain(s): 687, 2142

Witt, D. & Stackebrandt, E. 1988 Disproving the hypothesis of a common ancestry for the Ochromonas danica chrysoplast and Heliobacterium chlorum. Arch. Mikrobiol., 150, 244-248.
Strain(s): 2142
DOI: 10.1007/BF00407787

Coleman, A. W. 1988 The autofluorescent flagellum: a new phylogenetic enigma. J. Phycol., 24, 118-120.
Keywords: autofluorescence; Chrysophyceae; flagellum; fluorescence; heterokont; Ochromonas; photoreceptor; Prymnesium
Strain(s): 2142, 2144
DOI: 10.1111/j.1529-8817.1988.tb04465.x

Kawahara, T., Kumaki, Y., Kamada, T., Ishii, T., Okino, T. 2009 Absolute configuration of chlorosulfolipids from the chrysophyta Ochromonas danica. J. Org. Chem., 74, 6016-6024.
Strain(s): 2142
PubMed: 19627129
DOI: 10.1021/jo900860e

Yamamoto, Y., Kawashima, D., Hashizume, A., Hisamotsu, M., Isono, N. 2013 Purification and characterization of 1,3-β-D-glucan phosphorylase from Ochromonas danica. Biosci. Biotechnol. Biochem., 77, 130411-130416.
Keywords: 1,3-β-D-glucan phosphorylase; Ochromonas danica; laminarin; laminarioligosaccharides; 1,3-β-D-glucan
Strain(s): 2142
PubMed: 24018693
DOI: 10.1271/bbb.130411

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691
DOI: 10.60369/microresys.33.1_19

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

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