Strain Data

A big image opens when the thumbnail is clicked.(nies-2146.jpg)

Strain number		NIES-2146
Phylum		Heterokontophyta
Class		Eustigmatophyceae
Scientific name		Nannochloropsis oceanica Suda & Miyashita
Synonym
Former name		Nannochloropsis oculata (Droop) Hibberd
Common name
Locality (Date of collection)		Institute of Fisheries, Nagasaki, Nagasaki, Japan
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< IAM (2007) < Maruyama, Ko (around 1986)
Isolator (Date of isolation)		Maruyama, Ko (1986--)
Identified by		Re-identified by NIES
State of strain		Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: ESM ; ESM (agar) Temperature: 20 C Light intensity: 5-9 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M (liquid); 4 M (agar)
Gene information		18S rRNA ( LC730857 )
Cell size (min - max)		3 - 8 μm
Organization		Unicellular
Characteristics		Live food (Wullur et al. 2011)
Other strain no.		Other collection strain no. : IAM ST-6
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2145	Nannochloropsis oceanica		Marine	oil (hydrocarbon) production ; TAG (Nobusawa et al.)
NIES-2588	Nannochloropsis granulata		Marine (Seawater)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1388	Developayella elegans		Marine (Seawater)	Heterotrophic ; Phagotrophic
NIES-2351	Cylindrotheca fusiformis	Pennate diatom
NIES-2859	Ophiocytium sp.		Freshwater (Pond water)	Benthic
NIES-417	Asterionellopsis glacialis	Pennate diatom	Marine (Seawater)	Not growing under axenic condition
NIES-381	Eutreptiella gymnastica		Marine	Red tide ; Genome decoded strain

Reference
Maruyama, I., Nakamura, T., Matubayashi, T., Ando, Y., Maeda, T. 1986 Identification of the alga known as "marine Chlorella" as a member of the Eustigmatophyceae. Jpn. J. Phycol. (Sôrui), 34, 319-325. Strain(s): 2146 Wullur, S., Sakakura, Y., Hagiwara, A. 2011 Application of the minute monogonont rotifer Proales similis de Beauchamp in larval rearing of seven-band grouper epinephelus septemfasciatus. Aquaculture, 315, 355-360. Keywords: Rotifer; Proales similis; Brachionus rotundiformis; Live food; Fatty acid; Epinephelus septemfasciatus Strain(s): 2146 DOI: 10.1016/j.aquaculture.2011.02.025 Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89. Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860 PubMed: 24346654 DOI: 10.1007/s10265-013-0596-9 Shih, C., Chen, H., Lee, H., Tsai, H. 2015 Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata. PLoS One, 10, e0120780 (article ID). Strain(s): 2146 PubMed: 25793255 DOI: 10.1371/journal.pone.0120780 Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399. Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes Strain(s): 2145, 2146 PubMed: 29511798 DOI: 10.1007/s00299-018-2270-0 Shikata, T., Taniguchi, E., Sakamoto, S., Kitatsuji, S., Yamasaki, Y., Yoshida, M., Oikawa, H. 2020 Phylogeny, growth and toxicity of the noxious red-tide dinoflagellate Alexandrium leei in Japan. Reg. Stud. Mar. Sci., 36, 101265 (article ID). Keywords: Fish; Haemolytic activity; Photoinhibition; rDNA; Rotifer; Temperature Strain(s): 2146 DOI: 10.1016/j.rsma.2020.101265 Strain(s): 24, 47, 144, 2146, 2160 Sun, H., Wu, T., Chen, H Y., Ren, Y., Yang, S., Huang, J., Mou, H., Chen, F. 2021 Powerful tools for productivity improvements in microalgal production. Renew. Sust. Energ. Rev., 152, 111609 (article ID). Keywords: Microalgal production; Productivity improvement; Kinetic model; Metabolic flux analysis; Genetic tool; Lipid biosynthesis Strain(s): 144, 2140, 2146 DOI: 10.1016/j.rser.2021.111609 Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294. Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860 PubMed: 35672454 DOI: 10.1038/s41396-022-01264-1 Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62. Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392 DOI: 10.60369/microresys.38.2_51

Reference

Maruyama, I., Nakamura, T., Matubayashi, T., Ando, Y., Maeda, T. 1986 Identification of the alga known as "marine Chlorella" as a member of the Eustigmatophyceae. Jpn. J. Phycol. (Sôrui), 34, 319-325.
Strain(s): 2146

Wullur, S., Sakakura, Y., Hagiwara, A. 2011 Application of the minute monogonont rotifer Proales similis de Beauchamp in larval rearing of seven-band grouper epinephelus septemfasciatus. Aquaculture, 315, 355-360.
Keywords: Rotifer; Proales similis; Brachionus rotundiformis; Live food; Fatty acid; Epinephelus septemfasciatus
Strain(s): 2146
DOI: 10.1016/j.aquaculture.2011.02.025

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860
PubMed: 24346654
DOI: 10.1007/s10265-013-0596-9

Shih, C., Chen, H., Lee, H., Tsai, H. 2015 Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata. PLoS One, 10, e0120780 (article ID).
Strain(s): 2146
PubMed: 25793255
DOI: 10.1371/journal.pone.0120780

Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399.
Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes
Strain(s): 2145, 2146
PubMed: 29511798
DOI: 10.1007/s00299-018-2270-0

Shikata, T., Taniguchi, E., Sakamoto, S., Kitatsuji, S., Yamasaki, Y., Yoshida, M., Oikawa, H. 2020 Phylogeny, growth and toxicity of the noxious red-tide dinoflagellate Alexandrium leei in Japan. Reg. Stud. Mar. Sci., 36, 101265 (article ID).
Keywords: Fish; Haemolytic activity; Photoinhibition; rDNA; Rotifer; Temperature
Strain(s): 2146
DOI: 10.1016/j.rsma.2020.101265

Strain(s): 24, 47, 144, 2146, 2160

Sun, H., Wu, T., Chen, H Y., Ren, Y., Yang, S., Huang, J., Mou, H., Chen, F. 2021 Powerful tools for productivity improvements in microalgal production. Renew. Sust. Energ. Rev., 152, 111609 (article ID).
Keywords: Microalgal production; Productivity improvement; Kinetic model; Metabolic flux analysis; Genetic tool; Lipid biosynthesis
Strain(s): 144, 2140, 2146
DOI: 10.1016/j.rser.2021.111609

Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294.
Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860
PubMed: 35672454
DOI: 10.1038/s41396-022-01264-1

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392
DOI: 10.60369/microresys.38.2_51

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