Strain Data

Strain number		NIES-2163
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Auxenochlorella protothecoides (Krüger) Kalina & Puncochárová
Synonym		Chlorella protothecoides Krüger
Former name		Chlorella vulgaris Beijerinck
Common name		Green alga
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < BIU (UTEX);
Isolator (Date of isolation)		Pringsheim, E. G.
Identified by		Kessler, E.
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: Pro (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		18S-ITS1-5.8S-ITS2-28S ( KP645219 )
Cell size (min - max)		3 - 12 μm
Organization		Unicellular
Characteristics
Other strain no.		Other collection strain no. : IAM C-150; SAG 211-11a; UTEX 29; ATCC 30581; ATCC 13482; CCAP 211/11a; (BIU 29)
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2176	Auxenochlorella protothecoides	Green alga	(The freshwater sponge Spongilla fluviatalis)
NIES-2164	Auxenochlorella protothecoides	Green alga
NIES-2165	Auxenochlorella protothecoides	Green alga

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2233	Chlamydomonas pulvinata	Green alga	Freshwater (Water)	Phototaxis
NIES-2248	Chlamydomonas zebra	Green alga	Freshwater	Phototaxis
NIES-626	Pterosperma cristatum		Marine (Seawater)
NIES-687	Graesiella emersonii	Green alga		Starch production (Miyachi et al. 1986) ; Authentic strain of Chlorella fusca var. vacuolata Shihira & Krauss (Shihira & R.W.Krauss 1965)
NIES-2211	Chlamydomonas sp.	Green alga	Freshwater (Pool water)	Phototaxis

Reference
Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77. Strain(s): 226, 227, 685, 687, 2151, 2163, 2170, 2175 DOI: 10.1007/BF02489470 Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13. Strain(s): 227, 686, 2159, 2163, 2166, 2168, 2171, 2189, 2352 DOI: 10.1007/BF00690809 Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66. Strain(s): 226, 227, 685, 2151, 2163, 2170, 2175 DOI: 10.1007/BF02489469 Higashiyama, T. & Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195. Strain(s): 2163, 2166, 2171, 2189 PubMed: 1956777 DOI: 10.1093/nar/19.22.6191 Huss, V. A. R., Wein, K. H., Kessler, E. 1988 Deoxyribonucleic acid reassociation in the taxonomy of the genus Chlorella. Arch. Mikrobiol., 150, 509-511. Strain(s): 2163 Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553. Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176 DOI: 10.1111/j.0022-3646.1992.00550.x Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 2 protoplast induction and regeneration of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 279-286. Strain(s): 685, 2163, 2189 Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 3 interspecific cell fusion of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 287-293. Strain(s): 685, 2163, 2189 Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825. Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination Strain(s): 226, 227, 2159, 2163, 2165, 2169, 2175, 2189, 2208, 2211, 2213, 2214, 2229, 2231, 2233, 2240, 2241, 2243, 2247, 2248, 3277, 3284 DOI: 10.1007/s11274-004-8712-6 Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40. Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta Strain(s): 226, 250, 254, 550, 551, 626, 1298, 1332, 1703, 1836, 2137, 2163, 2164, 2165, 2166, 2252, 2353, 2894, 3377, 3645, 3867, 3891, 3892, 4107, 4390, 4452, 4696 DOI: 10.60369/microresys.39.1_33

Reference

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 226, 227, 685, 687, 2151, 2163, 2170, 2175
DOI: 10.1007/BF02489470

Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 227, 686, 2159, 2163, 2166, 2168, 2171, 2189, 2352
DOI: 10.1007/BF00690809

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 226, 227, 685, 2151, 2163, 2170, 2175
DOI: 10.1007/BF02489469

Higashiyama, T. & Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195.
Strain(s): 2163, 2166, 2171, 2189
PubMed: 1956777
DOI: 10.1093/nar/19.22.6191

Huss, V. A. R., Wein, K. H., Kessler, E. 1988 Deoxyribonucleic acid reassociation in the taxonomy of the genus Chlorella. Arch. Mikrobiol., 150, 509-511.
Strain(s): 2163

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176
DOI: 10.1111/j.0022-3646.1992.00550.x

Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 2 protoplast induction and regeneration of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 279-286.
Strain(s): 685, 2163, 2189

Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 3 interspecific cell fusion of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 287-293.
Strain(s): 685, 2163, 2189

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 226, 227, 2159, 2163, 2165, 2169, 2175, 2189, 2208, 2211, 2213, 2214, 2229, 2231, 2233, 2240, 2241, 2243, 2247, 2248, 3277, 3284
DOI: 10.1007/s11274-004-8712-6

Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 226, 250, 254, 550, 551, 626, 1298, 1332, 1703, 1836, 2137, 2163, 2164, 2165, 2166, 2252, 2353, 2894, 3377, 3645, 3867, 3891, 3892, 4107, 4390, 4452, 4696
DOI: 10.60369/microresys.39.1_33

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.