Strain Data

A big image opens when the thumbnail is clicked.(nies-2166.jpg)
Strain number NIES-2166  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Coccomyxa subellipsoidea E.Acton  
Synonym  
Former name Chlorella vulgaris Beijerinck < 'Chlorella' saccharophila (Krüger) Migula  
Common name Green alga  
Locality (Date of collection) Marble Point Antarctica  
Latitude / Longitude  
Habitat (Isolation source) (Dried algal peat)  
History < IAM (2007) < Holm-Hansen, O.  
Isolator (Date of isolation) Holm-Hansen, O.  
Identified by Kessler, E.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition) 		Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information Whole-genome ( AGSI00000000 )  
Cell size (min - max) 3 - 10 μm  
Organization Unicellular 
Characteristics Genome decoded strain (Blanc et al. 2012)  
Other strain no. Other collection strain no. : IAM C-169
Other strain no. : Holm-Hansen M-42-a  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2252
 		Coccomyxa dispar Green alga    
NIES-4343
 		Coccomyxa subellipsoidea Green alga   Terrestrial (The lichen Lichenomphalia meridionalis) Symbiotic (lichen)  
NIES-2353
 		Coccomyxa dispar Green alga   (Dried algal mat)  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2171
 		Micractinium inermum Green alga   Authentic strain (Hoshina et al. 2013)  
NIES-551
 		Cyanidium caldarium Red alga   Acidophilic ; Floridean starch (Shimonaga et al. 2007)  
NIES-2170
 		Chlorella vulgaris Green alga   Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)  
NIES-4452
 		Chloromonas fukushimae Green alga ; Snow alga   Terrestrial (Snow) Cryophilic ; Heterothallic ; Isogamous ; Mating type (+) ; Life cycle (Matsuzaki et al. 2020)  
NIES-1332
 		Cyanidioschyzon merolae Red alga   Hot spring (Water) Planktonic ; Acidophilic ; Thermophilic ; Genome decoded strain  
Reference
Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 227,  686,  2159,  2163,  2166,  2168,  2171,  2189,  2352 
DOI: 10.1007/BF00690809

Higashiyama, T. & Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195.
Strain(s): 2163,  2166,  2171,  2189 
PubMed: 1956777
DOI: 10.1093/nar/19.22.6191

Blanc, G., Agarkova, I., Grimwood, J., Kou, A., Brueggeman, A., Dunigan, D. D., Gurnon, J., Ladunga, I., Lindquist, E., Lucas, S., Pangilinan, J., Proschold, T., Salamov, A., Schmutz, J., Weeks, D., Yamada, T., Lomsadze, A., Borodovsky, M., Claverie, J. M., Grigoriev, I. V., Van Etten, J. L. 2012 The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation. Genome Biol., 13, R39 (article ID).
Strain(s): 2166 
PubMed: 22630137
DOI: 10.1186/gb-2012-13-5-r39

Smith, D. R., Burki, F., Yamada, T., Grimwood, J., Grigoriev, I. V., Van Etten, J. L., Keeling, P. J. 2011 The GC-rich mitochondrial and plastid genomes of the green alga Coccomyxa give insight into the evolution of organelle DNA nucleotide landscape. PLoS One, 6, e23624 (article ID).
Strain(s): 2166 
PubMed: 21887287
DOI: 10.1371/journal.pone.0023624

Lemieux, C., Otis, C.,Turmel, M. 2014 Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae. BMC Evol. Biol., 14, 211 (article ID).
Keywords: Chlorophyta; Trebouxiophyceae; Plastid genome; Phylogenomics
Strain(s): 1824,  2166 
PubMed: 25270575
DOI: 10.1186/s12862-014-0211-2

Darienko, T., Gustavs, L., Eggert, A., Wolf, W., Pröschold, T. 2015 Evaluating the species boundaries of green microalgae (Coccomyxa, Trebouxiophyceae, Chlorophyta) using integrative taxonomy and DNA barcoding with further implications for the species identification in environmental samples. PLoS One, 10, e0127838 (article ID).
Strain(s): 2166,  2252,  2353 
PubMed: 26080086
DOI: 10.1371/journal.pone.0127838

Peng, H., Wei, D., Chen, G., Chen, F. 2016 Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169. Biotechnol. Biofuels, 9, 151 (article ID).
Keywords: Coccomyxa subellipsoidea C-169; Elevated CO2; Lipid accumulation; Transcriptomic analysis; Phosphoenolpyruvate carboxylase; Pyruvate carboxylase; Carbamoyl-phosphate synthetase II; Ferredoxin; Vacuolar; H+-ATPase
Strain(s): 2166 
PubMed: 27453726
DOI: 10.1186/s13068-016-0571-5

Pfaff, S., Borchhardt, N., Boy, J., Karsten, Ulf., Gustavs, L. 2016 Desiccation tolerance and growth-temperature requirements of Coccomyxa (Trebouxiophyceae, Chlorophyta) strains from Antarctic biological soil crusts. Algol. Stud., 151/152, 3-19.
Keywords: Antarctica; biological soil crusts; ecophysiology; desiccation; cold adaptation; endemis
Strain(s): 2166 
DOI: 10.1127/algol_stud/2016/0245

Bertuzz, S., Gustavs, L., Pandolfini, G., Tretiac, M. 2017 Heat shock treatments for the control of lithobionts: A case study with epilithic green microalgae. Int. Biodeterior. Biodegrad., 123, 236-243.
Keywords: Biocides; Biodeteriogens; Chlorophyll a fluorescence; Hydration; Polyols; Non-reducing sugars
Strain(s): 2166 
DOI: 10.1016/j.ibiod.2017.06.023

Wang, C., Wang, Z., Luo, F., Li, Y. 2017 The augmented lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by nitrogen manipulation strategy. World J. Microbiol. Biotechnol., 33, 160 (article ID).
Keywords: Coccomyxa subellipsoidea; Nitrogen manipulation; Lipid; Fatty acids; Metabolites
Strain(s): 2166 
PubMed: 28752265
DOI: 10.1007/s11274-017-2324-4

Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, 7, e35122 (article ID).
Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis
Strain(s): 2166,  2235,  2541 
PubMed: 29848439
DOI: 10.7554/eLife.35122

Ranjan, P. & Kateriya, S. 2018 Localization and dimer stability of a newly identifed microbial rhodopsin from apolar, non-motile green algae. BMC Res. Notes, 11, 65 (article ID).
Keywords: Coccomyxa subellipsoidea; Proton-pumping rhodopsin; Optogenetics; Localization of the microbial rhodopsin in eukaryotes; Chlorella vulgaris
Strain(s): 2166 
PubMed: 29361974
DOI: 10.1186/s13104-018-3181-4

Zongyi, Y., Lu, L., Chen, J., Wei, D. 2018 Effect of crude glycerol on heterotrophic growth of Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169. J. Appl. Phycol., 30, 2989–2996.
Keywords: Chlorophyceae; Microalgae; Crude glycerol; Heterotrophic growth; Biodiesel
Strain(s): 2166 
DOI: 10.1007/s10811-018-1551-x

Kania, K., Zienkiewicz, M., Drożak, A. 2020 Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation. Protoplasma, 257, 607–611.
Keywords: Coccomyxa subellipsoidea C-169; Hygromycin B resistance; Electrotransformation; Electroporation; Stable genome transformation
Strain(s): 2166 
PubMed: 31741062
DOI: 10.1007/s00709-019-01447-2

Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., Kulikovskiy, M. 2019 Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorusconcentrations. J. Phycol., 55, 1154-1165.
Keywords: Coccomyxa; fatty acid profile lipid; nitrogen and phosphorus deprivation; Trebouxiophyceae
Strain(s): 2166,  2252,  2353 
PubMed: 31318981
DOI: 10.1111/jpy.12903

Hashizume, M., Yoshida, M., Demura, M., Watanabe, M. M. 2020 Culture study on utilization of phosphite by green microalgae. J. Appl. Phycol., 32, 889–899.
Keywords: Phosphite utilization; Chlorella vulgaris; Coccomyxa subellipsoidea
Strain(s): 2166,  2170,  2280 
DOI: 10.1007/s10811-020-02088-2

Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., Kulikovskiy, M. 2021 A new species of freshwater algae Nephrochlamys yushanlensis sp. nov. (Selenastraceae, Sphaeropleales) and its lipid accumulation during nitrogen and phosphorus starvation. J. Phycol., 57, 606-618.
Strain(s): 2166 
PubMed: 33296071
DOI: 10.1111/jpy.13116

Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 226,  250,  254,  550,  551,  626,  1298,  1332,  1703,  1836,  2137,  2163,  2164,  2165,  2166,  2252,  2353,  2894,  3377,  3645,  3867,  3891,  3892,  4107,  4390,  4452,  4696 
DOI: 10.60369/microresys.39.1_33

Liu, Y. & Wei, D. 2023 Enhancing carbon dioxide fixation and co-production of protein and lutein in oleaginous Coccomyxa subellipsoidea by a stepwise light intensity and nutrients feeding strategy. Bioresour. Technol., 376, 28885 (article ID).
Keywords: Coccomyxa subellipsoidea; CO2 fixation; Protein; Lutein; Stepwise feeding
Strain(s): 2166 
PubMed: 36925078
DOI: 10.1016/j.biortech.2023.128885

Tsai, P.-C., Kato, K., Shen, J.-R., Akita, F. 2025 Structural study of the chlorophyll between Lhca8 and PsaJ in an Antarctica green algal photosystem I-LHCI supercomplex revealed by its atomic structure. Biochim. Biophys. Acta-Bioenerg., 1866, 149543 (article ID).
Keywords: Photosystem I; Light harvesting; Coccomyxa subellipsoidea; Membrane protein complex; Excitation transfer pathway
Strain(s): 2166 
PubMed: 39947506
DOI: 10.1016/j.bbabio.2025.149543

Liu, Y. & Wei, D. 2023 High-efficient CO2-to-protein bioconversion by oleaginous Coccomyxa subellipsoidea using light quality shift and nitrogen supplementation strategy. Chem. Eng. J., 473, 145166 (article ID).
Keywords: Coccomyxa subellipsoidea; CO2 fixation; Protein production; Light quality shift; Nitrogen supplementation; Transcription analysis
Strain(s): 2166 
DOI: 10.1016/j.cej.2023.145166

Yang, R., Chen, G., Peng, H., Wei, D. 2019 Identification and Characterization of MiRNAs in Coccomyxa subellipsoidea C-169. Int. J. Mol. Sci., 20, 3448 (article ID).
Keywords: Coccomyxa subellipsoidea C-169; miRNA; lipid metabolism; transcription/translation factor; CO2 supplementation
Strain(s): 2166 
PubMed: 31337051
DOI: 10.3390/ijms20143448

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