Strain Data

Strain number		NIES-194
Phylum		Streptophyta
Class		Zygnematophyceae
Scientific name		Closterium spinosporum Hodgetts var. spinosporum
Synonym
Former name
Common name		Desmid
Locality (Date of collection)		Tsukude, Aichi, Japan (1972-10-**)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Pond water)
History		< Watanabe, Masayuki
Isolator (Date of isolation)		Ichimura, Terunobu
Identified by		Watanabe, Masayuki
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: CAM Temperature: 20 (25) C Light intensity: 4-8 (12) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (1 M)
Gene information		18S rRNA ( AF352224 ) , ITS1-5.8S-ITS2 ( AF419989 ) , 26S rRNA ( AF419990 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Homothallic
Other strain no.		Other strain no. : A-2-22
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-228	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229
NIES-229	Closterium ehrenbergii	Desmid	Freshwater (Paddy soil)	Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228
NIES-67	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (+) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-68	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Pool soil)	Heterothallic: Mating type (−) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)
NIES-172	Closterium moniliferum	Desmid		Homothallic

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-65	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Lake water)	Heterothallic: Mating type (+) ; Mating group II B

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-92	Tetradesmus acuminatus	Green alga	Freshwater (Lake water)
NIES-634	Carteria eugametos	Green alga	Freshwater
NIES-664	Pleurotaenium nodosum	Desmid	Freshwater (Bog water)
NIES-104	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Moat water)	Cyanobacterial water bloom (aoko) ; ST11 (Tanabe et al. 2007)
NIES-305	Pseudanabaena sp.	Blue-green alga ; Cyanobacteria	Freshwater (Sediment)

Reference
Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072. Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales Strain(s): 52, 125, 165, 170, 174, 175, 180, 181, 185, 187, 188, 191, 194, 198, 199, 200, 339, 449 DOI: 10.1046/j.1529-8817.2001.00146.x Ichimura, T. & Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14. Strain(s): 163, 164, 165, 168, 186, 187, 194, 339, 340, 341 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Watanabe, M. 1978 A taxonomic study of the Closterium calosporum complex (1). Bull. Natn. Sci. Mus., Ser. B (Bot.), 4, 133-154, pls. 1-8. Strain(s): 163, 164, 165, 168, 171, 186, 187, 188, 189, 191, 192, 193, 194, 197, 339, 341 Watanabe, M. 1979 A taxonomic study of the Closterium calosporum complex (2). Bull. Natn. Sci. Mus., Ser. B (Bot.), 5, 1-23, pls. 9-11. Strain(s): 163, 164, 165, 168, 186, 187, 188, 189, 191, 192, 194, 197, 339, 340, 341

Reference

Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072.
Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales
Strain(s): 52, 125, 165, 170, 174, 175, 180, 181, 185, 187, 188, 191, 194, 198, 199, 200, 339, 449
DOI: 10.1046/j.1529-8817.2001.00146.x

Ichimura, T. & Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14.
Strain(s): 163, 164, 165, 168, 186, 187, 194, 339, 340, 341

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Watanabe, M. 1978 A taxonomic study of the Closterium calosporum complex (1). Bull. Natn. Sci. Mus., Ser. B (Bot.), 4, 133-154, pls. 1-8.
Strain(s): 163, 164, 165, 168, 171, 186, 187, 188, 189, 191, 192, 193, 194, 197, 339, 341

Watanabe, M. 1979 A taxonomic study of the Closterium calosporum complex (2). Bull. Natn. Sci. Mus., Ser. B (Bot.), 5, 1-23, pls. 9-11.
Strain(s): 163, 164, 165, 168, 186, 187, 188, 189, 191, 192, 194, 197, 339, 340, 341

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