Strain Data

Strain number		NIES-447
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Gloeomonas anomalipyrenoides Nakada, Matsuzaki & Nozaki
Synonym
Former name		Chloromonas insignis (Anachin) Gerloff & Ettl
Common name		Green alga
Locality (Date of collection)		Lake Kasumigaura, Tsuchiura, Ibaraki, Japan (1983-08-22)
Latitude / Longitude		36.081204 / 140.214193
Habitat (Isolation source)		Freshwater (Lake water)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1983-08-24)
Identified by		Nakada, Takashi (Reidentify)
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 3 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA ( AB504776 AB624567 ) , 18S-ITS1-5.8S-ITS2-28S ( AB971362 ) , ITS2 ( AB624578 ) , atpB ( AB084313 ) , psaA ( AB624593 ) , psaB ( AB084348 ) , rbcL ( AB022226 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Planktonic ; Authentic strain (Nakada et al. 2015)
Other strain no.		Other strain no. : Kas-8
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-464	Gloeomonas lateperforata	Green alga	Freshwater (Pond water)
NIES-3640	Gloeomonas anomalipyrenoides	Green alga	Freshwater (Lake water)	Planktonic

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-34	Phormidium foveolarum	Blue-green alga ; Cyanobacteria
NIES-35	Raphidocelis subcapitata	Green alga	Freshwater	Test strain for AGP ; Genome decoded strain (Suzuki et al. 2018)
NIES-55	Closterium peracerosum-strigosum-littorale complex	Desmid	Freshwater (Paddy water)	Heterothallic: Mating type (−) ; Mating group II C
NIES-647	Gonium quadratum	Green alga	Freshwater	Phototaxis ; Heterothallic ; Isogamous ; Mating type (−) ; F1 clone of NIES-652 x 653 ; Sister clone to NIES-648, 649 and 650 from one zygote
NIES-298	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater	Cyanobacterial water bloom (aoko) ; Toxicity ; microcystin (+) (Tanabe et al. 2009b) ; ST60 (Tanabe et al. 2007) ; Draft genome sequence (Rhee et al. 2012)

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO₂-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372. Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase Strain(s): 447 DOI: 10.1007/s004250050571 Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201. Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales Strain(s): 447, 464 DOI: 10.1111/j.1529-8817.2009.00773.x Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85. Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales Strain(s): 447 DOI: 10.2216/11-18.1 Nakada, T., Matsuzaki, R., Krienitz, L., Tomiwa, M., Nozaki, H. 2015 Taxonomic reassessment of strains formerly classified as Chloromonas insignis (Volvocales, Chlorophyceae) and description of Gloeomonas anomalipyrenoides sp. nov. Acta Phytotax. Geobot., 66, 23-33. Keywords: 18S rRNA; Chloromonas; culture strain; Gloeomonas; light microscopy; new species; phycology; phylogeny Strain(s): 447, 3640, 3641, 3642 DOI: 10.18942/apg.KJ00009868503 Watanabe, S., Nozaki, H., Nakada, T., Lewis, L. A. 2016 Comparative ultrastructural analysis of Chloromonas and Gloeomonas (Chlorophyceae): tracing the origin of Gloeomonas-specific basal apparatus traits. Phycologia, 55, 585-601. Keywords: Chloromonadinia; Coarsely striated proximal fiber (CSPF); Distal connecting fiber-associated layered structure (DFLS); Gloeomonas rubrifilum comb. nov.; Ixipapillifera; Morphological novelty Strain(s): 447, 464 DOI: 10.2216/15-147.1 Barcytė, D. & Eliáš, M. 2025 Characterization of Lercomonas mokradensis gen. et sp. nov. enriches the phylogenetic and taxonomic diversity of Chloromonadinia (Chlorophyceae). Phycologia, 64, 71-83. Strain(s): 447, 464, 2215, 2243, 2379, 2563, 2862, 3389, 3707, 3753, 4284, 4476 DOI: 10.1080/00318884.2025.2471620

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO₂-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372.
Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase
Strain(s): 447
DOI: 10.1007/s004250050571

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201.
Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales
Strain(s): 447, 464
DOI: 10.1111/j.1529-8817.2009.00773.x

Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85.
Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales
Strain(s): 447
DOI: 10.2216/11-18.1

Nakada, T., Matsuzaki, R., Krienitz, L., Tomiwa, M., Nozaki, H. 2015 Taxonomic reassessment of strains formerly classified as Chloromonas insignis (Volvocales, Chlorophyceae) and description of Gloeomonas anomalipyrenoides sp. nov. Acta Phytotax. Geobot., 66, 23-33.
Keywords: 18S rRNA; Chloromonas; culture strain; Gloeomonas; light microscopy; new species; phycology; phylogeny
Strain(s): 447, 3640, 3641, 3642
DOI: 10.18942/apg.KJ00009868503

Watanabe, S., Nozaki, H., Nakada, T., Lewis, L. A. 2016 Comparative ultrastructural analysis of Chloromonas and Gloeomonas (Chlorophyceae): tracing the origin of Gloeomonas-specific basal apparatus traits. Phycologia, 55, 585-601.
Keywords: Chloromonadinia; Coarsely striated proximal fiber (CSPF); Distal connecting fiber-associated layered structure (DFLS); Gloeomonas rubrifilum comb. nov.; Ixipapillifera; Morphological novelty
Strain(s): 447, 464
DOI: 10.2216/15-147.1

Barcytė, D. & Eliáš, M. 2025 Characterization of Lercomonas mokradensis gen. et sp. nov. enriches the phylogenetic and taxonomic diversity of Chloromonadinia (Chlorophyceae). Phycologia, 64, 71-83.
Strain(s): 447, 464, 2215, 2243, 2379, 2563, 2862, 3389, 3707, 3753, 4284, 4476
DOI: 10.1080/00318884.2025.2471620

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