Strain Data

Strain number		NIES-213
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Pediastrum duplex Meyen var. duplex
Synonym		Pediastrum napoleonis Ralfs; Pediastrum pertusum Kützing; Pediastrum duplex f. genuinum Arnold & Aleksenko; Pediastrum selenaea Kützing 1845; Pediastrum pertusum var. genuinum A.Braun 1855; Pediastrum duplex var. reticulatum Lagerheim 1882; Pediastrum duplex var. clathratum (A.Braun) Lagerheim 1882; Pediastrum duplex var. genuinum (A.Braun) Lagerheim 1882
Former name
Common name		Green alga
Locality (Date of collection)		Tsukuba, Ibaraki, Japan (1983-05-25)
Latitude / Longitude		36.105319 / 140.081738
Habitat (Isolation source)		Freshwater (Pond water)
History		< Hiwatari, Takehiko
Isolator (Date of isolation)		Hiwatari, Takehiko (1983-06-14)
Identified by		Hiwatari, Takehiko; Watanabe, Masayuki (Reidentify)
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		atpB ( AB084306 ) , rbcL ( AB084333 ) , psaB ( AB084340 ) , Pcy ( AB218890 )
Cell size (min - max)
Organization
Characteristics
Other strain no.		Other strain no. : AQ-P-1
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-301	Pediastrum boryanum	Green alga	Freshwater (Lake water)
NIES-211	Pediastrum duplex	Green alga	Freshwater (Lake water)
NIES-212	Pediastrum duplex	Green alga	Freshwater (Lake water)
NIES-214	Pediastrum duplex	Green alga	Freshwater (Pond water)
NIES-215	Pediastrum simplex	Green alga	Freshwater (Lake water)

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-300	Pediastrum angulosum	Green alga	Freshwater (Lake water)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-667	Yamagishiella unicocca	Green alga	Freshwater (Pond water)	Phototaxis ; Heterothallic ; Isogamous ; Mating type (−) ; Crosses with NIES-666
NIES-628	Astrephomene gubernaculifera	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Isogamous ; Mating type (−)
NIES-248	Cosmocladium constrictum	Desmid	Freshwater (Lake water)
NIES-506	Phormidium jenkelianum	Blue-green alga ; Cyanobacteria	Freshwater (River sediment)
NIES-303	Penium margaritaceum	Desmid	Freshwater (Ditch water)

Reference
Hiwatari, T., Kasai, F., Watanabe, M. M., Nei, T. 1984 Cryopreservation of Scenedesmus acutus and Pediastrum duplex in liquid nitrogen - Survival and growth after freezing. Jpn. J. Freez. Dry., 30, 27-31 (in Japanese with English title). Strain(s): 95, 213 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579 Nakamura, M. 2019 Reciprocal expression of PCY1 and CYC6 in Pseudopediastrum boryanum and Pediastrum duplex (Chlorophyceae) is regulated via a common transcription mechanism under copper-deficient condition. Toho Liberal Arts Review, 50, 81-97. Keywords: Chlorophyceae; Copper response elements; Cytochrome _c6; Gene expression; Hydrodictyaceae; GTAC motif; Pediastrum; Plastocyanin Strain(s): 213, 301 DOI: 10.14994/toho.liberal.arts.rev.50.81 Nakamura, M. 2022 Plastocyanin and cytochrome _c6 from green algae. Toho Liberal Arts Review, 54, 105-116 (in Japanese). Strain(s): 213, 215, 301, 2863 DOI: 10.14994/toho.liberal.arts.rev.54.105

Reference

Hiwatari, T., Kasai, F., Watanabe, M. M., Nei, T. 1984 Cryopreservation of Scenedesmus acutus and Pediastrum duplex in liquid nitrogen - Survival and growth after freezing. Jpn. J. Freez. Dry., 30, 27-31 (in Japanese with English title).
Strain(s): 95, 213

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Nakamura, M. 2019 Reciprocal expression of PCY1 and CYC6 in Pseudopediastrum boryanum and Pediastrum duplex (Chlorophyceae) is regulated via a common transcription mechanism under copper-deficient condition. Toho Liberal Arts Review, 50, 81-97.
Keywords: Chlorophyceae; Copper response elements; Cytochrome _c6; Gene expression; Hydrodictyaceae; GTAC motif; Pediastrum; Plastocyanin
Strain(s): 213, 301
DOI: 10.14994/toho.liberal.arts.rev.50.81

Nakamura, M. 2022 Plastocyanin and cytochrome _c6 from green algae. Toho Liberal Arts Review, 54, 105-116 (in Japanese).
Strain(s): 213, 215, 301, 2863
DOI: 10.14994/toho.liberal.arts.rev.54.105

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