Strain Data

Strain number		NIES-30
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Leptolyngbya sp.
Synonym
Former name		Phormidium tenue (C.Agardh ex Gomont) Anagnostidis et Komárek
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Akita, Akita, Japan
Latitude / Longitude		39.710407 / 140.232038
Habitat (Isolation source)		Freshwater (Paddy field)
History		< IAM (1983);
Isolator (Date of isolation)		Ishikawa, Masako
Identified by		Homma, Takamitsu (Reidentify)
State of strain		Cryopreservation; Unialgal; Non-clonal; Non-axenic
Culture condition (Preculture condition)		Medium: MDM (agar) Temperature: 20 (25) C Light intensity: 4-10 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M (14 D)
Gene information		Whole-genome ( MRCG00000000 ) , 16S rRNA ( AB042857 )
Cell size (min - max)		1 - 3 μm
Organization		Filamentous
Characteristics		Genome decoded strain (Zhu et al. 2017)
Other strain no.		Other collection strain no. : IAM M-40
Remarks		Cryopreserved
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2104	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance ; Genome decoded strain (Shimura et al. 2015)
NIES-2129	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria
NIES-3755	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial (Soil)	Genome decoded strain (Hirose et al. 2016)
NIES-2118	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria
NIES-2103	Leptolyngbya sp.	Blue-green alga ; Cyanobacteria	Terrestrial	Drought tolerance

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2135	Leptolyngbya boryana	Blue-green alga ; Cyanobacteria		Genome decoded strain

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-646	Gonium pectorale	Green alga	Freshwater (Pond sediment)	Phototaxis ; Heterothallic ; Isogamous ; Mating type (−)
NIES-359	Oltmannsiellopsis unicellularis		Marine (Seawater)	Red tide ; Authentic strain
NIES-129	Coelastrum astroideum	Green alga	Freshwater
NIES-532	Stigeoclonium fasciculare	Green alga	Freshwater (Lake water)
NIES-2102	Cylindrospermum muscicola	Blue-green alga ; Cyanobacteria

Reference
Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary). Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees Strain(s): 21, 30, 33, 41, 73, 76, 512 DOI: 10.11532/proes1992.37.183 Shinobu, K., Abe, S., Asano, K., Abe, T. 1992 On the laboratory experiment of algal culturing (AGP-test) [Sôrui baiyô no shitsunai jikken (AGP shiken) ni tsuite]. Heisei 4-nendo Tôhoku Chihô Kensetsu-kyoku Kan-nai Gijutsu Kenkyû Happyôkai Shiryô, Kyô-28, 151-154 (in Japanese). Strain(s): 30 Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermidine Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277 Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116 DOI: 10.1016/j.hal.2012.01.003 Oikawa, E. Ishibashi, Y., 2004 Species speciﬁcity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46. Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree Strain(s): 21, 30, 33, 41, 73, 76, 512, 611 PubMed: 15237605 DOI: 10.2166/wst.2004.0529 Zhu, T., Hou, S., Lu, X., Hess, W. R. 2017 Draft genome sequences of nine cyanobacterial strains from diverse habitats. GenomeA, 5, e01676-16 (article ID). Strain(s): 30, 208, 592, 593, 1031, 2101, 2119, 2130 PubMed: 28254973 DOI: 10.1128/genomeA.01676-16 Lin, W., Zhao, D., Luo, J. 2018 Distribution of alkaline phosphatase genes in cyanobacteria and the role of alkaline phosphatase on the acquisition of phosphorus from dissolved organic phosphorus for cyanobacterial growth. J. Appl. Phycol., 30, 839-850. Keywords: Alkaline; phosphatase; Cyanobacteria; Dissolved organic phosphorus; Distribution Strain(s): 30, 39, 81, 2130, 2549, 3756 DOI: 10.1007/s10811-017-1267-3 Gómez-Lojero, C., Leyva-Castillo, L. E., Herrera-Salgado, P., Barrera-Rojas, J., Ríos-Castro, E., Gutiérrez-Cirlos, E. B. 2018 Leptolyngbya CCM 4, a cyanobacterium with far-red photoacclimation from Cuatro Ciénegas Basin, México. Photosynthetica, 56, 342–353. Keywords: chlorophyll f; Cuatro Ciénegas Basin; far-red light; photosynthesis; phycobilisomes. Strain(s): 30 DOI: 10.1007/s11099-018-0774-z MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451. Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106 PubMed: 31899489 DOI: 10.1093/molbev/msz308

Reference

Ichimura, T. & Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary).
Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees
Strain(s): 21, 30, 33, 41, 73, 76, 512
DOI: 10.11532/proes1992.37.183

Shinobu, K., Abe, S., Asano, K., Abe, T. 1992 On the laboratory experiment of algal culturing (AGP-test) [Sôrui baiyô no shitsunai jikken (AGP shiken) ni tsuite]. Heisei 4-nendo Tôhoku Chihô Kensetsu-kyoku Kan-nai Gijutsu Kenkyû Happyôkai Shiryô, Kyô-28, 151-154 (in Japanese).
Strain(s): 30

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116
DOI: 10.1016/j.hal.2012.01.003

Oikawa, E. Ishibashi, Y., 2004 Species speciﬁcity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46.
Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree
Strain(s): 21, 30, 33, 41, 73, 76, 512, 611
PubMed: 15237605
DOI: 10.2166/wst.2004.0529

Zhu, T., Hou, S., Lu, X., Hess, W. R. 2017 Draft genome sequences of nine cyanobacterial strains from diverse habitats. GenomeA, 5, e01676-16 (article ID).
Strain(s): 30, 208, 592, 593, 1031, 2101, 2119, 2130
PubMed: 28254973
DOI: 10.1128/genomeA.01676-16

Lin, W., Zhao, D., Luo, J. 2018 Distribution of alkaline phosphatase genes in cyanobacteria and the role of alkaline phosphatase on the acquisition of phosphorus from dissolved organic phosphorus for cyanobacterial growth. J. Appl. Phycol., 30, 839-850.
Keywords: Alkaline; phosphatase; Cyanobacteria; Dissolved organic phosphorus; Distribution
Strain(s): 30, 39, 81, 2130, 2549, 3756
DOI: 10.1007/s10811-017-1267-3

Gómez-Lojero, C., Leyva-Castillo, L. E., Herrera-Salgado, P., Barrera-Rojas, J., Ríos-Castro, E., Gutiérrez-Cirlos, E. B. 2018 Leptolyngbya CCM 4, a cyanobacterium with far-red photoacclimation from Cuatro Ciénegas Basin, México. Photosynthetica, 56, 342–353.
Keywords: chlorophyll f; Cuatro Ciénegas Basin; far-red light; photosynthesis; phycobilisomes.
Strain(s): 30
DOI: 10.1007/s11099-018-0774-z

MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G. 2020 Origin and evolution of carboxysome positioning systems in cyanobacteria. Mol. Biol. Evol., 37, 1434–1451.
Strain(s): 21, 22, 25, 26, 30, 39, 44, 73, 87, 88, 267, 592, 806, 843, 932, 970, 2098, 2100, 2107, 2108, 2111, 2549, 3275, 3754, 3756, 3974, 4073, 4074, 4075, 4101, 4103, 4106
PubMed: 31899489
DOI: 10.1093/molbev/msz308

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