Strain number NIES-2666  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Kamigo Saitama, Japan (2004-08-08)  
Latitude / Longitude 36.2692 / 139.1331 
Habitat (Isolation source) Freshwater (Paddy water)  
History < Sekimoto, Hiroyuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2004-08-10)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  22 C
Light intensity:  11-14 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 5.8S-ITS2-28S ( AB625611 ) , 18S rRNA ( AB477426 ) , CpPI-kd01 ( AB558136 ) , CpPI-kd02 ( AB558137 ) , CpPI-kd03 ( AB558138 ) , CpPI-kd04 ( AB558139 ) , CpPI-kd05 ( AB558140 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Homothallic ; Isogamous  
Other strain no. Other strain no. : kodama20  
Remarks  
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-228
Closterium ehrenbergii Desmid   Freshwater (Paddy soil) Heterothallic ; Mating type (+) ; Mating group B ; Crossing with NIES-229  
NIES-229
Closterium ehrenbergii Desmid   Freshwater (Paddy soil) Heterothallic ; Mating type (−) ; Mating group B ; Crossing with NIES-228  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-62
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (+) ; Mating group II A  
NIES-59
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (+) ; Mating group II A  
NIES-4319
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Pond water) Heterothallic ; Isogamou ; Mating type (−) ; Mating group G (Tsuchikane et al. 2018)  
NIES-61
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (−) ; Mating group II B  
NIES-63
Closterium peracerosum-strigosum-littorale complex Desmid   Freshwater (Paddy water) Heterothallic: Mating type (−) ; Mating group II A  
Reference
Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 585960616263646567686970261266645814582 
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Kokubun, Y., Sekimoto, H. 2010 Characterization and molecular cloning of conjugation-regulating sex pheromones in homothallic Closterium. Plant Cell Physiol., 51, 1515-1523.
Keywords: Closterium; Conjugation; Homothallic; Homothallism; Sex pheromone; Sexual reproduction; Zygospore
Strain(s): 2666 
PubMed: 20656896
DOI: 10.1093/pcp/pcq110

Tsuchikane, Y., Sato, M., Ootaki, T., Kokubun, Y., Nozaki, H., Ito, M., Sekimoto, H. 2010 Sexual prosesses and phylogenetic relationship of a homothallic strain in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 46, 278-284.
Keywords: Closterium; conjugation; heterothallic; pheromone; sexual reproduction
Strain(s): 2666 
DOI: 10.1111/j.1529-8817.2010.00806.

Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 535458596263646568261266643194320432145484550455145524581458245834584 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Watanabe, M., Kawaguchi, Y. W., Uehara, K., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2024 Diversity of genome size and chromosome number in homothallic and heterothallic strains of the Closterium peracerosum–strigosum–littorale complex (Desmidiales, Zygnematophyceae, Streptophyta). J. Phycol., 60, 654-667.
Keywords: Closterium; flow cytometry; genome size; heterothallism; homothallism; sexual reproduction
Strain(s): 5253545859606162636465676826126664319432043214322454845494550455145524581458245834584 
PubMed: 38678594
DOI: 10.1111/jpy.13457

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