Strain Data

Strain number		NIES-2722
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Fistulifera saprophila (Lange-Bertalot) Lange-Bertalot
Synonym
Former name
Common name		Pennate diatom
Locality (Date of collection)		Sakagawa River, Tsukuba, Ibaraki, Japan (2005-06-27)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Stone)
History		< Mayama, Shigeki
Isolator (Date of isolation)		Ishihara, Satoru (2005-07-10)
Identified by		Mayama, Shigeki
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: CSi Temperature: 15 C Light intensity: 5-8 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA ( AB769958 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Test strain for AGP etc. (Ishihara, S. et al. 2006)
Other strain no.		Other strain no. : K10-3
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1	Chattonella marina		Marine (Seawater)	Red tide
NIES-223	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine	Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-324	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Seawater)	Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-2844	Skeletonema tropicum	Centric diatom	Marine (Seawater)	Red tide (Yamada et al. 2010) ; Planktonic
NIES-407	Achnanthidium minutissimum	Pennate diatom	Freshwater (River water)

Reference
Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A., Mayama, S. 2006 Development of a growth inhibition test method using fresh water attached diatoms. Jpn. J. Environ. Toxicol., 9, 101-113 (in Japanese with English summary). Keywords: attached diatom; growth inhibition test; herbicide; ecological risk assessment Strain(s): 71, 2717, 2718, 2719, 2720, 2721, 2722, 2724, 2725, 2726, 2727, 2728, 2729, 2730, 2731, 2732, 2733 DOI: 10.11403/jset.9.101 Ishihara, S. 2008 Development of risk assessment pocedure for evaluating effect of herbicides on primary productivity of river ecosystem. Bull. Nat. Inst. Agro-Env. Sci., 25, 1-92 (in Japanese with English summary). Strain(s): 2717, 2718, 2719, 2720, 2721, 2722, 2723, 2724, 2725, 2726, 2727, 2728, 2729, 2730, 2731, 2732, 2733 Matsumoto, M., Mayama, S., Nemoto, M., Fukuda, Y., Muto, M., Yoshino, T., Matsunaga, T., Tanaka, T. 2014 Morphological and molecular phylogenetic analysis of the high triglyceride-producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae). Phycol. Res., 62, 257-268. Keywords: 18S rDNA; JPCC DA0580; marine diatom; morphometric analyses Strain(s): 2722 DOI: 10.1111/pre.12066 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 DOI: 10.60369/microresys.33.1_19

Reference

Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A., Mayama, S. 2006 Development of a growth inhibition test method using fresh water attached diatoms. Jpn. J. Environ. Toxicol., 9, 101-113 (in Japanese with English summary).
Keywords: attached diatom; growth inhibition test; herbicide; ecological risk assessment
Strain(s): 71, 2717, 2718, 2719, 2720, 2721, 2722, 2724, 2725, 2726, 2727, 2728, 2729, 2730, 2731, 2732, 2733
DOI: 10.11403/jset.9.101

Ishihara, S. 2008 Development of risk assessment pocedure for evaluating effect of herbicides on primary productivity of river ecosystem. Bull. Nat. Inst. Agro-Env. Sci., 25, 1-92 (in Japanese with English summary).
Strain(s): 2717, 2718, 2719, 2720, 2721, 2722, 2723, 2724, 2725, 2726, 2727, 2728, 2729, 2730, 2731, 2732, 2733

Matsumoto, M., Mayama, S., Nemoto, M., Fukuda, Y., Muto, M., Yoshino, T., Matsunaga, T., Tanaka, T. 2014 Morphological and molecular phylogenetic analysis of the high triglyceride-producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae). Phycol. Res., 62, 257-268.
Keywords: 18S rDNA; JPCC DA0580; marine diatom; morphometric analyses
Strain(s): 2722
DOI: 10.1111/pre.12066

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691
DOI: 10.60369/microresys.33.1_19

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