Strain Data

Strain number		NIES-2842
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Skeletonema menzelii Guillard, Carpenter & Reimann
Synonym
Former name
Common name		Centric diatom
Locality (Date of collection)		Dokai Bay, Kitakyushu, Fukuoka, Japan (2008-09-10)
Latitude / Longitude		33.88388888 / 130.78722222
Habitat (Isolation source)		Marine (Seawater)
History		< Yamada, Machiko
Isolator (Date of isolation)		Yamada, Machiko (2008--)
Identified by		Yamada, Machiko
State of strain		Subculture; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: ESM Temperature: 15 C Light intensity: 3-10 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 21 D
Gene information		28S rRNA ( AB572830 ) , CO1 ( AB948163 )
Cell size (min - max)
Organization
Characteristics		Planktonic
Other strain no.		Other strain no. : FDK222
Remarks
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-16	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Sediment)	Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-2537	Skeletonema tropicum	Centric diatom	Marine (Seawater)	Red tide ; Planktonic
NIES-323	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Seawater)	Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-324	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Seawater)	Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-2838	Skeletonema costatum	Centric diatom	Marine (Seawater)	Red tide (Yamada et al. 2010) ; Planktonic

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3698	Skeletonema sp.	Centric diatom	Marine (Seawater)	Red tide ; Planktonic
NIES-2840	Skeletonema grevillei	Centric diatom	Marine (Seawater)	Planktonic

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-1975	Chattonella marina		Marine (Seawater)	Red tide
NIES-377	Chaetoceros socialis	Centric diatom	Marine (Seawater)
NIES-589	Odontella aurita	Centric diatom	Marine (Seawater)	Red tide
NIES-2697	Emiliania huxleyi	Coccolithophore	Marine (Seawater)	Planktonic
NIES-277	Cryptomonas rostratiformis		Freshwater (Pond water)

Reference
Yamada, M., Katsuki, E., Otsubo, M., Kawaguchi, M., Ichimi, K., Keriyama, H., Tada, K., Harrison, P. J. 2010 Species diversity of the genus Skeltonema (Bacillariophyceae) in the Industrial Harbor Dokai Bay, Japan. J. Oceanogr., 66, 755-771. Keywords: Skeletonema; species diversity; isolation characteristics; Dokai Bay; LSU rDNA; SSU rDNA; SEM Strain(s): 2837, 2838, 2839, 2841, 2842, 2843, 2844 DOI: 10.1007/s10872-010-0062-4 Kaeriyama, H., Katsuki, E., Otsubo, M., Yamada, K., Ichimi, K., Tada, K., Harrison, P. J. 2011 Effects of temperature and irradiance on growth of strains belonging to seven Skeletonema species isolated from Dokai Bay, southern Japan. Eur. J. Phycol., 46, 113-124. Keywords: Dokai Bay; irradiance; physiology; Skeletonema species; specific growth rate; temperature Strain(s): 2837, 2838, 2839, 2841, 2842, 2843, 2844 DOI: 10.1080/09670262.2011.565128 Kawachi, M., Kataoka, T., Sato, M., Noël, M.-H., Kuwata, A., Demura, M., Yamaguchi, H. 2016 Application of cryopreservation to genetic analyses of a photosynthetic picoeukaryote community. Gene, 576, 708-716. Keywords: 18S rRNA gene; 454 pyros equencing; Cryopreservation; Photosynthetic picoeukaryotes Strain(s): 765, 1966, 2842 PubMed: 26506442 DOI: 10.1016/j.gene.2015.10.033 Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225. Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA Strain(s): 16, 223, 2837, 2838, 2842 DOI: 10.1111/pre.12179 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 DOI: 10.60369/microresys.33.1_19

Reference

Yamada, M., Katsuki, E., Otsubo, M., Kawaguchi, M., Ichimi, K., Keriyama, H., Tada, K., Harrison, P. J. 2010 Species diversity of the genus Skeltonema (Bacillariophyceae) in the Industrial Harbor Dokai Bay, Japan. J. Oceanogr., 66, 755-771.
Keywords: Skeletonema; species diversity; isolation characteristics; Dokai Bay; LSU rDNA; SSU rDNA; SEM
Strain(s): 2837, 2838, 2839, 2841, 2842, 2843, 2844
DOI: 10.1007/s10872-010-0062-4

Kaeriyama, H., Katsuki, E., Otsubo, M., Yamada, K., Ichimi, K., Tada, K., Harrison, P. J. 2011 Effects of temperature and irradiance on growth of strains belonging to seven Skeletonema species isolated from Dokai Bay, southern Japan. Eur. J. Phycol., 46, 113-124.
Keywords: Dokai Bay; irradiance; physiology; Skeletonema species; specific growth rate; temperature
Strain(s): 2837, 2838, 2839, 2841, 2842, 2843, 2844
DOI: 10.1080/09670262.2011.565128

Kawachi, M., Kataoka, T., Sato, M., Noël, M.-H., Kuwata, A., Demura, M., Yamaguchi, H. 2016 Application of cryopreservation to genetic analyses of a photosynthetic picoeukaryote community. Gene, 576, 708-716.
Keywords: 18S rRNA gene; 454 pyros equencing; Cryopreservation; Photosynthetic picoeukaryotes
Strain(s): 765, 1966, 2842
PubMed: 26506442
DOI: 10.1016/j.gene.2015.10.033

Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225.
Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA
Strain(s): 16, 223, 2837, 2838, 2842
DOI: 10.1111/pre.12179

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691
DOI: 10.60369/microresys.33.1_19

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