Strain Data

Strain number		NIES-2860
Phylum		Heterokontophyta
Class		Eustigmatophyceae
Scientific name		Vacuoliviride crystalliferum T.Nakayama, T.Nakamura, A.Yokoyama, T.Shiratori, I.Inouye & Ishida
Synonym
Former name
Common name
Locality (Date of collection)		inside Tsukuba University, Tsukuba, Ibaraki, Japan (1999--)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Paste)
History		< Inouye, Isao
Isolator (Date of isolation)		Inouye, Isao (1999--)
Identified by		Nakayama, Takeshi
State of strain		Cryopreservation; Unialgal; Axenic[2018 Jan]
Culture condition (Preculture condition)		Medium: AF-6 Temperature: 20 C Light intensity: 23 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA ( LC004696 ) , rbcL ( MZ336043 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Benthic ; Authentic strain (Nakayama et al. 2015)
Other strain no.		Other strain no. : nak-9
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-4277	Vacuoliviride crystalliferum		Freshwater (Water)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-276	Cryptomonas borealis		Freshwater (Pond water)	Genome decoded strain (Suzuki et al. 2022)
NIES-1388	Developayella elegans		Marine (Seawater)	Heterotrophic ; Phagotrophic
NIES-1435	Unidentified coccoid prasinophyte		Marine (Seawater)
NIES-417	Asterionellopsis glacialis	Pennate diatom	Marine (Seawater)	Not growing under axenic condition
NIES-286	Euglena mutabilis		Freshwater (Sediment)	Bioindicator

Reference
Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89. Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860 PubMed: 24346654 DOI: 10.1007/s10265-013-0596-9 Nakayama, T., Nakamura, A., Yokoyama, A., Shiratori, T., Inouye, I., Ishida, K-I. 2015 Taxonomic study of a new eustigmatophycean alga, Vacuoliviride crystalliferum gen. et so. nov. J. Plant Res., 128, 249-257. Keywords: Eustigmatophyceae; Phylogeny; Taxonomy; Vacuoliviride crystalliferum gen. et so. nov. Strain(s): 2860 PubMed: 25516501 DOI: 10.1007/s10265-014-0686-3 Shiraiwa, Y., Inouye, I., Ishida, K. 2014 放射性同位元素の除去剤および除去方法 In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Tokukai2014-6051, 30 pp. Strain(s): 2854, 2855, 2856, 2857, 2858, 2859, 2860 Kim, T. Y., Hong, J. E., Park, H. M., Lee, U. J., Lee, S-Y. 2020 Decontamination of low-level contaminated water from radioactive cesium and cobalt using microalgae. J. Radioanal. Nucl. Chem., 323, 903-908. Keywords: Radionuclide; Microalgae; Cobalt; Cesium; Decontamination; Biosorption Strain(s): 2860 DOI: 10.1007/s10967-019-07008-4 Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730. Strain(s): 2285, 2587, 2860 PubMed: 33293415 DOI: 10.1073/pnas.2005460117 Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294. Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860 PubMed: 35672454 DOI: 10.1038/s41396-022-01264-1 Přibyl, P. & MProcházková, L. 2023 Trebonskia zoosporica, gen. et sp. nov., a new member of the Goniochloridales (Eustigmatophyceae, Stramenopiles) with an unusual mode of reproduction. Eur. J. Phycol., 58, 199-213. Keywords: 18S rRNA; Eustigmatophyceae; fatty acids; Goniochloridales; rbcL; Trebonskia zoosporica; zoospores Strain(s): 2860 DOI: 10.1080/09670262.2022.2089913 Fawley, M. W., Fawley, K. P., Cahoon, A. B. 2021 Finding needles in a haystack—Extensive diversity in the eustigmatophyceae revealed by community metabarcode analysis targeting the rbcL gene using lineage-directed primers. J. Phycol., 57, 1636-1647. Keywords: algal diversity; community structure; environmental DNA; Eustigmatophyceae; metabar-coding; species diversity Strain(s): 2860 PubMed: 34218435 DOI: 10.1111/jpy.13196 Khudzari, J., Fukuda, S., Shiraiwa, Y., Iwamoto, K. 2022 Kinetic properties of ¹³⁷Cs uptake by the cesium‑accumulating eustigmatophycean microalga. Environ. Sci. Pollut. Res., 30, 34460–34467. Keywords: ¹³⁷Cs; Radioactive cesium-accumulating mechanism; Cesium-accumulating microalgae; Vacuoliviride crystalliferum (eustigmatophyceae); Radiocesium phycoremediation Strain(s): 2860 PubMed: 36509956 DOI: 10.1007/s11356-022-24573-6 Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62. Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392 DOI: 10.60369/microresys.38.2_51

Reference

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 24, 36, 71, 155, 160, 203, 233, 246, 329, 333, 391, 405, 417, 440, 487, 503, 529, 531, 538, 548, 553, 587, 597, 678, 727, 805, 843, 859, 931, 995, 1003, 1012, 1015, 1017, 1031, 1044, 1045, 1259, 1326, 1331, 1377, 1382, 1388, 1411, 1435, 1439, 1440, 1441, 1442, 1458, 1728, 1826, 1831, 1833, 1840, 1846, 1862, 1865, 1956, 2131, 2144, 2146, 2147, 2150, 2175, 2176, 2185, 2268, 2325, 2341, 2352, 2377, 2412, 2437, 2854, 2855, 2856, 2858, 2859, 2860
PubMed: 24346654
DOI: 10.1007/s10265-013-0596-9

Nakayama, T., Nakamura, A., Yokoyama, A., Shiratori, T., Inouye, I., Ishida, K-I. 2015 Taxonomic study of a new eustigmatophycean alga, Vacuoliviride crystalliferum gen. et so. nov. J. Plant Res., 128, 249-257.
Keywords: Eustigmatophyceae; Phylogeny; Taxonomy; Vacuoliviride crystalliferum gen. et so. nov.
Strain(s): 2860
PubMed: 25516501
DOI: 10.1007/s10265-014-0686-3

Shiraiwa, Y., Inouye, I., Ishida, K. 2014 放射性同位元素の除去剤および除去方法 In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Tokukai2014-6051, 30 pp.
Strain(s): 2854, 2855, 2856, 2857, 2858, 2859, 2860

Kim, T. Y., Hong, J. E., Park, H. M., Lee, U. J., Lee, S-Y. 2020 Decontamination of low-level contaminated water from radioactive cesium and cobalt using microalgae. J. Radioanal. Nucl. Chem., 323, 903-908.
Keywords: Radionuclide; Microalgae; Cobalt; Cesium; Decontamination; Biosorption
Strain(s): 2860
DOI: 10.1007/s10967-019-07008-4

Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730.
Strain(s): 2285, 2587, 2860
PubMed: 33293415
DOI: 10.1073/pnas.2005460117

Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294.
Strain(s): 67, 303, 995, 1604, 2146, 2285, 2860
PubMed: 35672454
DOI: 10.1038/s41396-022-01264-1

Přibyl, P. & MProcházková, L. 2023 Trebonskia zoosporica, gen. et sp. nov., a new member of the Goniochloridales (Eustigmatophyceae, Stramenopiles) with an unusual mode of reproduction. Eur. J. Phycol., 58, 199-213.
Keywords: 18S rRNA; Eustigmatophyceae; fatty acids; Goniochloridales; rbcL; Trebonskia zoosporica; zoospores
Strain(s): 2860
DOI: 10.1080/09670262.2022.2089913

Fawley, M. W., Fawley, K. P., Cahoon, A. B. 2021 Finding needles in a haystack—Extensive diversity in the eustigmatophyceae revealed by community metabarcode analysis targeting the rbcL gene using lineage-directed primers. J. Phycol., 57, 1636-1647.
Keywords: algal diversity; community structure; environmental DNA; Eustigmatophyceae; metabar-coding; species diversity
Strain(s): 2860
PubMed: 34218435
DOI: 10.1111/jpy.13196

Khudzari, J., Fukuda, S., Shiraiwa, Y., Iwamoto, K. 2022 Kinetic properties of ¹³⁷Cs uptake by the cesium‑accumulating eustigmatophycean microalga. Environ. Sci. Pollut. Res., 30, 34460–34467.
Keywords: ¹³⁷Cs; Radioactive cesium-accumulating mechanism; Cesium-accumulating microalgae; Vacuoliviride crystalliferum (eustigmatophyceae); Radiocesium phycoremediation
Strain(s): 2860
PubMed: 36509956
DOI: 10.1007/s11356-022-24573-6

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392
DOI: 10.60369/microresys.38.2_51

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