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Strain number NIES-3351  
Phylum Dinophyta  
Class Dinophyceae  
Scientific name Ostreopsis cf. ovata  
Synonym  
Former name  
Common name Dinoflagellate  
Locality (Date of collection) Issai, Otsuki, Kochi, Japan (2009-08-06)  
Latitude / Longitude 32.787319078 / 132.635553303 
Habitat (Isolation source) Marine (Seaweed)  
History < Sato, Shinya  
Isolator (Date of isolation) Sato, Shinya (2009-08-07)  
Identified by Sato, Shinya  
State of strain Subculture; Unialgal; Clonal; Non-aAxenic[2023 Novt]  
Culture condition
(Preculture condition)
Medium:  MNKf/2  
Temperature:  20 C
Light intensity:  20-24 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information  
Cell size (min - max) - 37 μm  
Organization Unicellular 
Characteristics Toxicity (Sato et al. 2011) ; clade A (Sato et al. 2011) ; Confirmed same sequence with LSU rDNA D8-D10 (Accession No. AB674776) of IshiOst61 (Sato et al. 2011)  
Other strain no. Other strain no. : s0752  
Remarks Toxicity; Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Sato, S., Nishimura, T., Uehara, K., Sakanari, H., Tawong, W., Hariganeya, N., Smith, K., Rhodes, L., Yasumoto, T., Taira, Y., Suda, S., Yamaguchi, H., Adachi, M. 2011 Phylogeography of Ostreopsis along West Pacific coast, with special reference to a novel clade from Japan. PLoS One, 6, e27983 (article ID).
Strain(s): 3351 
PubMed: 22164222
DOI: 10.1371/journal.pone.0027983

Suzuki, T., Watanabe, R., Uchida, H., Matsushima, R., Nagai, H., Yasumoto, T., Yoshimatsu, T., Sato, S., Adachi, M. 2012 LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan. Harmful Algae, 20, 81-91.
Keywords: Novel ovatoxin isomers; Ostreopsis; Palytoxin analogues; LC-MS/MS
Strain(s): 3351 
DOI: 10.1016/j.hal.2012.08.002

Poli, M., Ruiz-Olvera, P., Nalca, A., Ruiz, S., Livingston, V., Frick, O., Dyer, D., Schellhase C., Raymond, J., Kulis, D., Anderson, D., McGrath, S., Deeds, J. 2018 Toxicity and pathophysiology of palytoxin congeners after intraperitoneal and aerosol administration in rats. Toxicon, 150, 235-250.
Keywords: Palytoxin congeners; Intraperitoneal administration; Aerosol administration; Lethality; Tissue histopathology; Rats
Strain(s): 3351 
PubMed: 29902540
DOI: 10.1016/j.toxicon.2018.06.067

D'Arelli, A., Carlin, M., Sosa, S., Melchiorre, C., Varriale, F., Tartaglione, L., Varra, M., Kulis, D., Anderson, D. M., Poli, M. 2025 Cutaneous effects of ovatoxin-a: an in vitro study on human skin keratinocytes. Arch. Toxicol., 100, 773-788.
Keywords: Ovatoxin-a; Ostreopsis cf. ovata; Palytoxin; Cutaneous toxicity; Mechanism of action
Strain(s): 3351 
PubMed: 41171426
DOI: 10.1007/s00204-025-04229-3

Yoshioka, Y., Ando, C., Yamashita, H., Kawamitsu, M., Kawachi, M., Tsunematsu, Y., Shoguchi, E. 2026 A dataset for forty complete bacterial genome sequences in cultures of the toxic dinoflagellate Ostreopsis cf. ovata. Data Brief, 65, 112499 (article ID).
Keywords: Alphaproteobacteria; Gammaproteobacteria; Bacteroidia; Rhodothermia; Actinomycete; Metagenome assembly; NIES-3351
Strain(s): 3351 
PubMed: 41704501
DOI: 10.1016/j.dib.2026.112499

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