Strain Data

Strain number		NIES-3374
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Chlorogonium capillatum Nozaki, M.M.Watanabe & Aizawa
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Osaka, Japan
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Pond water)
History		< Suzaki, Toshinobu
Isolator (Date of isolation)		Suzaki, Toshinobu (1988--)
Identified by		Suzaki, Toshinobu
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Jan]
Culture condition (Preculture condition)		Medium: AF-6 Temperature: 20 C Light intensity: 11-17 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		act1 ( AB973441 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Mixotrophic ; Osmotrophic ; Planktonic ; Prey for protozoa (flagellates, ciliates, amoebids, heliozoans)
Other strain no.		Other strain no. : ATCC 50936
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2296	Chlorogonium complexum	Green alga	Freshwater (Soil)	Authentic strain (Nakada et al. 2010) ; Homothallic ; Isogamous ; Resting spore-forming
NIES-755	Chlorogonium euchlorum	Green alga		Authentic strain ; Type specimen (NIES-50003, Epitype)
NIES-743	Chlorogonium capillatum	Green alga	(Soil)
NIES-692	Chlorogonium capillatum	Green alga	Freshwater (Bog soil)	Authentic strain ; Monoecious ; Isogamou ; Paedogamy ; Type specimen (NIES-50001, Epitype)
NIES-1357	Chlorogonium elongatum	Green alga	Freshwater (Paddy soil)	Mixotrophic ; Osmotrophic ; Planktonic ; Cosmopolitan

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-751	Chlorogonium elongatum	Green alga	Freshwater (Pond water)	Authentic strain ; Type specimen (NIES-50002, Epitype)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-8	Chrysotila roscoffensis	Coccolithophore	Marine	Red tide
NIES-351	Eudorina elegans	Green alga	Freshwater (Lake water)	Phototaxis ; Homothallic
NIES-2300	Ochromonas sp.		Freshwater (Pond sediment)	Mixotrophic ; Phagotrophic
NIES-1334	Kathablepharis japonica		Marine (Seawater)	Heterotrophic ; Phagotrophic
NIES-2677	Bigelowiella natans		Marine (Seawater)	Planktonic

Reference
Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae. J. Gen. Appl. Micobiol., 59, 313-319. Keywords: green alga; homospermidine; polyamine; thermospermine Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374 PubMed: 24005181 DOI: 10.2323/jgam.59.313 Sakaguchi, M., Murakami, H., Suzaki, T. 2001 Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Protist, 152, 33-41. Strain(s): 2497, 3374 PubMed: 11401035 DOI: 10.1078/1434-4610-00041 Kinoshita, E., Suzaki, T., Sugiyama, M., Shigenaka, Y. 1995 Ultrastructure and rapid axopodial contraction of a heliozoan, Raphidiophyrys contractilis sp. nov. J. Eucaryot. Microbiol., 42, 283-288. Keywords: Contraction; Heliozoa; microtubule pattern; new species; Raphidiophyrys; silicic scale; X-ray microanalysis Strain(s): 2498, 3374 DOI: 10.1111/j.1550-7408.1995.tb01581.x Sakaguchi, M., Suzuki, T., Khan, S. M. M. K. Hausmann, K. 2002 Food capture by kinetocysts in the heliozoon Raphidiophrys contractilis. Eur. J. Protistol., 37, 453-458. Keywords: Exocytosis; Extrusome; Heliozoa; Kinetocyst Strain(s): 2498, 3374 DOI: 10.1078/0932-4739-00847 Sakaguchi, M., Suzuki, T. 1999 Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol., 35, 1-10. Keywords: Actinophrys sol; Antibiotic; Heliozoa; Monoxenic Culture Strain(s): 2497, 3374 DOI: 10.1016/S0932-4739(99)80050-9 Song, C., Suzaki, T. 2013 Improved preservation of organelies in Paramecium bursaria by freeze-substitution with glutaraldehyde and osmium tetroxide. J. Electr. Microsc. Technol. Med. Biol., 27, 1-8. Keywords: Paramecium bursaria; endosymbiosis; cryofixation; ultrastructural preservation; freeze-substitution Strain(s): 3374 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. 2015 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Plant Syst. Evol., 301, 1725-1733. Keywords: Volvocales; Divergent actin Strain(s): 351, 546, 576, 717, 3374 DOI: 10.1007/s00606-014-1187-5 Higuchi, S., Sugahara, F., Pascual-Anaya, J., Takagi, W., Oisi, Y., Kuratani, S. 2019 Inner ear development in cyclostomes and evolution of the vertebrate semicircular canals. Nature, 565, 347–350. Strain(s): 3374 PubMed: 30518864 DOI: 10.1038/s41586-018-0782-y Higuchi, R., Song, C., Hoshina, R., Suzaki, T. 2018 Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea). Eur. J. Protistol., 66, 149-155. Keywords: Cell wall; Chlorella variabilis; GlycosaminoglycansParamecium bursaria; Symbiosis Strain(s): 3374 PubMed: 30286318 DOI: 10.1016/j.ejop.2018.09.003 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Hoshina, R., Tsukii, Y., Harumoto, T., Suzaki, T. 2021 Characterization of a green Stentor with symbiotic algae growing in an extremely oligotrophic environment and storing large amounts of starch granules in its cytoplasm. Sci Rep, 11, 2865 (article ID). Strain(s): 3374 PubMed: 33536497 DOI: 10.1038/s41598-021-82416-9 Azuma, T., Pánek, T., Tice, A. K., Kayama, M., Kobayashi, M., Miyashita, H., Suzaki, T., Yabuki, A., Brown, M. W., Kamikawa, R. 2022 An enigmatic stramenopile sheds light on early evolution in Ochrophyta plastid organellogenesis. Mol. Biol. Evol., 39, msac065 (article ID). Keywords: Actinophryidae; plastid evolution; aminoacyl-tRNA synthase; gene transfer; phylogenomics; organellar DNA Strain(s): 2497, 3374 PubMed: 35348760 DOI: 10.1093/molbev/msac065

Reference

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae. J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Sakaguchi, M., Murakami, H., Suzaki, T. 2001 Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Protist, 152, 33-41.
Strain(s): 2497, 3374
PubMed: 11401035
DOI: 10.1078/1434-4610-00041

Kinoshita, E., Suzaki, T., Sugiyama, M., Shigenaka, Y. 1995 Ultrastructure and rapid axopodial contraction of a heliozoan, Raphidiophyrys contractilis sp. nov. J. Eucaryot. Microbiol., 42, 283-288.
Keywords: Contraction; Heliozoa; microtubule pattern; new species; Raphidiophyrys; silicic scale; X-ray microanalysis
Strain(s): 2498, 3374
DOI: 10.1111/j.1550-7408.1995.tb01581.x

Sakaguchi, M., Suzuki, T., Khan, S. M. M. K. Hausmann, K. 2002 Food capture by kinetocysts in the heliozoon Raphidiophrys contractilis. Eur. J. Protistol., 37, 453-458.
Keywords: Exocytosis; Extrusome; Heliozoa; Kinetocyst
Strain(s): 2498, 3374
DOI: 10.1078/0932-4739-00847

Sakaguchi, M., Suzuki, T. 1999 Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol., 35, 1-10.
Keywords: Actinophrys sol; Antibiotic; Heliozoa; Monoxenic Culture
Strain(s): 2497, 3374
DOI: 10.1016/S0932-4739(99)80050-9

Song, C., Suzaki, T. 2013 Improved preservation of organelies in Paramecium bursaria by freeze-substitution with glutaraldehyde and osmium tetroxide. J. Electr. Microsc. Technol. Med. Biol., 27, 1-8.
Keywords: Paramecium bursaria; endosymbiosis; cryofixation; ultrastructural preservation; freeze-substitution
Strain(s): 3374

Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. 2015 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Plant Syst. Evol., 301, 1725-1733.
Keywords: Volvocales; Divergent actin
Strain(s): 351, 546, 576, 717, 3374
DOI: 10.1007/s00606-014-1187-5

Higuchi, S., Sugahara, F., Pascual-Anaya, J., Takagi, W., Oisi, Y., Kuratani, S. 2019 Inner ear development in cyclostomes and evolution of the vertebrate semicircular canals. Nature, 565, 347–350.
Strain(s): 3374
PubMed: 30518864
DOI: 10.1038/s41586-018-0782-y

Higuchi, R., Song, C., Hoshina, R., Suzaki, T. 2018 Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea). Eur. J. Protistol., 66, 149-155.
Keywords: Cell wall; Chlorella variabilis; GlycosaminoglycansParamecium bursaria; Symbiosis
Strain(s): 3374
PubMed: 30286318
DOI: 10.1016/j.ejop.2018.09.003

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775, 4477, 4478
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Hoshina, R., Tsukii, Y., Harumoto, T., Suzaki, T. 2021 Characterization of a green Stentor with symbiotic algae growing in an extremely oligotrophic environment and storing large amounts of starch granules in its cytoplasm. Sci Rep, 11, 2865 (article ID).
Strain(s): 3374
PubMed: 33536497
DOI: 10.1038/s41598-021-82416-9

Azuma, T., Pánek, T., Tice, A. K., Kayama, M., Kobayashi, M., Miyashita, H., Suzaki, T., Yabuki, A., Brown, M. W., Kamikawa, R. 2022 An enigmatic stramenopile sheds light on early evolution in Ochrophyta plastid organellogenesis. Mol. Biol. Evol., 39, msac065 (article ID).
Keywords: Actinophryidae; plastid evolution; aminoacyl-tRNA synthase; gene transfer; phylogenomics; organellar DNA
Strain(s): 2497, 3374
PubMed: 35348760
DOI: 10.1093/molbev/msac065

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