Strain Data

A big image opens when the thumbnail is clicked.(nies-0255.jpg)

Strain number		NIES-255
Phylum		Chlorophyta
Class		Mamiellophyceae
Scientific name		Monomastix minuta Skuja
Synonym
Former name
Common name
Locality (Date of collection)		Tsuchiura, Ibaraki, Japan (1983-07-22)
Latitude / Longitude		36.081096 / 140.16337
Habitat (Isolation source)		Freshwater (Pond water)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1983-07-22)
Identified by		Suda, Shoichiro
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Jan]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 24-39 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563091 ) , 18S-ITS1-5.8S-ITS2-28S ( FN562446 )
Cell size (min - max)		3 - 12 μm
Organization		Unicellular; Flagellate
Characteristics
Other strain no.		Other strain no. : SIS-Mono
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-256	Monomastix minuta		Freshwater (Bog water)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3403	Pyramimonas sp.		Marine (Sand)
NIES-3398	Pyramimonas sp.		Marine (Sand)
NIES-102	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Toxicity ; microcystin (+) (Tanabe et al. 2009b) ; Genome decoded strain (Yamaguchi et al. 2020) ; ST10 (Tanabe et al. 2007)
NIES-275	Cryptomonas ovata		Freshwater (Pond water)
NIES-363	Pedinomonas minor		Freshwater (Paddy soil)

Reference
Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83. Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298 DOI: 10.1016/0269-7491(93)90180-V Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295. Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536 Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251. Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates Strain(s): 255, 483, 485 PubMed: 16171190 DOI: 10.1016/j.protis.2005.04.003 Marin, B., Melkonian, M. 2010 Molecular phylogeny and classiﬁcation of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336. Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae Strain(s): 252, 255, 360, 483 PubMed: 20005168 DOI: 10.1016/j.protis.2009.10.002 Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae. J. Gen. Appl. Micobiol., 59, 313-319. Keywords: green alga; homospermidine; polyamine; thermospermine Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374 PubMed: 24005181 DOI: 10.2323/jgam.59.313 Suda, S., Bhuiyan, M. A. H., Faria, D. G. 2013 Genetic diversity of Pyramimonas from Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales). J. Mar. Sci. Tech., 21, 285-296. Keywords: Pyramimonas; nuclear SSU rDNA; genetic diversity; Ryukyu Archipelago Strain(s): 221, 251, 255, 3392, 3393, 3394, 3396, 3397, 3398, 3399, 3401, 3403, 3405, 3407, 3408, 3411, 3413, 3414, 3416, 3417, 3418 DOI: 10.6119/JMST-013-1220-16

Reference

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298
DOI: 10.1016/0269-7491(93)90180-V

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18, 251, 254, 255, 296, 329, 483, 486, 536

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255, 483, 485
PubMed: 16171190
DOI: 10.1016/j.protis.2005.04.003

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classiﬁcation of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252, 255, 360, 483
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae. J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Suda, S., Bhuiyan, M. A. H., Faria, D. G. 2013 Genetic diversity of Pyramimonas from Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales). J. Mar. Sci. Tech., 21, 285-296.
Keywords: Pyramimonas; nuclear SSU rDNA; genetic diversity; Ryukyu Archipelago
Strain(s): 221, 251, 255, 3392, 3393, 3394, 3396, 3397, 3398, 3399, 3401, 3403, 3405, 3407, 3408, 3411, 3413, 3414, 3416, 3417, 3418
DOI: 10.6119/JMST-013-1220-16

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.