Strain number NIES-3708  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Geminocystis sp.  
Synonym  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Mimomi Hongo Park, Narashino, Chiba, Japan (2013-11-01)  
Latitude / Longitude 35.684195 / 140.064770 
Habitat (Isolation source) Freshwater (Lake water)  
History < Hirose, Yuu < Katayama, Mitsunori  
Isolator (Date of isolation) Katayama, Mitsunori (2013-11-01)  
Identified by Hirose, Yuu  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  BG-11  
Temperature:  20 C
Light intensity:  6-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information Whole-genome ( AP014815 )  
Cell size (min - max)  
Organization  
Characteristics Phototaxis ; chromatic adaptation ; Genome decoded strain (Hirose et al. 2015a)  
Other strain no. Other strain no. : CIT003  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-1643
Sphaerospermopsis aphanizomenoides Blue-green alga ; Cyanobacteria   Freshwater (Pond water) Planktonic  
NIES-3755
Leptolyngbya sp. Blue-green alga ; Cyanobacteria   Terrestrial (Soil) Genome decoded strain (Hirose et al. 2016)  
NIES-932
Raphidiopsis curvata Blue-green alga ; Cyanobacteria   Freshwater (Pond water) Cyanobacterial water bloom (aoko) ; Planktonic ; Gram negative ; Genome decoded strain  
NIES-39
Arthrospira platensis Blue-green alga ; Cyanobacteria   Salt water Autotrophic ; Genome decoded strain (Fujisawa et al. 2010) ; Hydrogen evolution ; Contains good quality of proteins  
NIES-3736
Planktothrix suspensa Blue-green alga ; Cyanobacteria   Freshwater (Lake water)  
Reference
Hirose, Y., Katayama, M., Ohtsubo, Y., Misawa, N., Iioka, E., Suda, W., Oshima, K., Hanaoka, M., Tanaka, K., Eki, T., Ikeuchi, M., Kikuchi, Y., Ishida, M., Hattori, M. 2015a Complete genome sequence of cyanobacterium Geminocystis sp. strain NIES-3708, which performs type II complementary chromatic acclimation. GenomeA, 3, e00357-15 (article ID).
Strain(s): 3708 
PubMed: 25953174
DOI: 10.1128/genomeA.00357-15

Hirose, Y., Misawa, N., Yonekawa, C., Nagao, N., Watanabe, M., Ikeuchi, M., Eki, T. 2017 Characterization of the genuine type 2 chromatic acclimation in the two Geminocystis cyanobacteria. DNA Res., 24, 387-396.
Keywords: Cyanobacteria; chromatic acclimation; phycobilisome; cyanobacteriochrome
Strain(s): 3708,  3709 
PubMed: 28338901
DOI: 10.1093/dnares/dsx011

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27,  39,  42,  73,  81,  87,  89,  100,  101,  102,  103,  104,  105,  107,  111,  112,  207,  208,  230,  266,  298,  416,  433,  478,  510,  512,  515,  592,  593,  604,  611,  808,  809,  835,  843,  846,  847,  905,  928,  931,  932,  945,  981,  989,  1025,  1043,  1058,  1061,  1064,  1068,  1069,  1071,  1076,  1085,  1099,  1101,  1105,  1113,  1115,  1126,  1133,  1134,  1135,  1144,  1157,  1215,  1642,  1643,  1652,  1668,  1672,  1673,  1677,  1678,  1698,  2087,  2131,  2412,  2665,  3708,  3733,  3734,  3736,  3755,  3756,  3757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Ohbayashi, R., Hirooka, S., Onuma, R., Kanesaki, Y., Hirose, Y., Kobayashi, Y., Fujiwara, T., Furusawa, C., Miyagishima, S. 2020 Evolutionary changes in DnaA-dependent chromosomal replication in cyanobacteria. Front. Microbiol., 11, 786 (article ID).
Keywords: CDS skew; chromosome replication; cyanobacteria; DnaA, GC skew; polyploidy
Strain(s): 3708 
PubMed: 32411117
DOI: 10.3389/fmicb.2020.00786

Karimi-Fard, A., Saidi, A., Tohidfar, M., Emami, S. N. 2025 Integrative bioinformatics approaches reveal key hub genes in cyanobacteria: insights from Synechocystis sp. PCC 6803 and Geminocystis sp. NIES-3708 under abiotic stress conditions. Genes Genom., 47, 383–397.
Keywords: Environmental stresses; Meta-analysis; WGCNA; Machine learning; Gene expression; Cyanobacteria
Strain(s): 3708 
PubMed: 39849193
DOI: 10.1007/s13258-025-01615-0

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