Strain Data
Strain number NIES-3714  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Chaetoceros tenuissimus Meunier  
Synonym  
Former name  
Common name Centric diatom  
Locality (Date of collection) Ohno-Seto, Seto Inland Sea, Hatsukaichi, Hiroshima, Japan (2002-07-09)  
Latitude / Longitude 34.275251 / 132.26701 
Habitat (Isolation source) Marine (Seawater)  
History < Kimura, Kei < Tomaru, Yuji  
Isolator (Date of isolation) Tomaru, Yuji (2002-07-09)  
Identified by Tomaru, Yuji  
State of strain Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition) 		Medium:  mSWM-3  
Temperature:  15 C
Light intensity:  9-11 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information endogenous viral fragment ( LC650338 LC650339 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Red tide ; The virus which infects with this diatom strain has been reported (Tomaru et al. 2010).  
Other strain no. Other collection strain no. : CCAP 1010/42
Other strain no. : 2-6  
Remarks Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-586
 		Chaetoceros didymus Centric diatom   Marine (Seawater) Red tide ; Test strain (Kohira, M.., et al. 2016)  
NIES-3715
 		Chaetoceros tenuissimus Centric diatom   Marine (Seawater) Red tide ; Genome decoded strain (Hongo et al. 2021) ; The virus which infects with this diatom strain has been reported (Shirai et al. 2008).  
NIES-3710
 		Chaetoceros debilis Centric diatom   Marine (Seawater) Red tide ; Resting spore-forming ; The virus which infects with this diatom strain has been reported (Tomaru, Y. et al. 2008).  
NIES-3711
 		Chaetoceros lorenzianus Centric diatom   Marine (Seawater) Red tide ; The virus which infects with this diatom strain has been reported (Tomaru et al. 2011).  
NIES-3713
 		Chaetoceros socialis Centric diatom   Marine (Seawater) Red tide ; The virus which infects with this diatom strain has been reported (Tomaru et al. 2009).  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-3712
 		Chaetoceros setoensis Centric diatom   Marine (Seawater) Red tide ; The virus which infects with this diatom strain has been reported (Tomaru et al. 2013).  
NIES-3716
 		Chaetoceros sp. Centric diatom   Marine (Seawater) Red tide ; The virus which infects with this diatom strain has been reported (Tomaru et al. 2012).  
NIES-324
 		Skeletonema marinoi-dohrnii complex Centric diatom   Marine (Seawater) Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade  
Reference
Tomaru, Y., Shirai, Y., Toyoda, K., Nagasaki, K. 2011 Isolation and characterisation of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus. Aquat. Microb. Ecol., 64, 175-184.
Keywords: Bacilladnavirus; Chaetoceros; Diatom; ssDNA; Virus; Rod-shaped particle
Strain(s): 3714,  3715 
DOI: 10.3354/ame01517

Kimura, K. & Tomaru, Y. 2017 Effects of temperature and salinity on diatom cell lysis by DNA and RNA viruses. Aquat. Microb. Ecol., 79, 79-83.
Keywords: Water temperature; Water salinity; Host-virus interactions; Diatom lysis
Strain(s): 3714,  3716 
DOI: 10.3354/ame01818

Tomaru, Y., Toyoda, K., Kimura, K. 2018 Occurrence of the planktonic bloom-forming marine diatom Chaetoceros tenuissimus Meunier and its infectious viruses in western Japan. Hydrobiologia, 805, 221-230.
Keywords: Algal viruses; Primary producers; Marine algae; Real-time quantitative PCR; Restingstage cells
Strain(s): 3714,  3715 
DOI: 10.1007/s10750-017-3306-0

Tomaru, Y. & Kimura, K. 2020 Novel protocol for estimating viruses specifically infecting the marine planktonic diatoms. Diversity-Basel, 12, 225 (article ID).
Keywords: marine diatom; Chaetoceros tenuissimus; Chaetoceros setoensis; host-virus specificity; RdRp; ssRNA virus; ssDNA virus; viral genome
Strain(s): 3712,  3714,  3715 
DOI: 10.3390/d12060225

Yamaguchi, H., Sumida, N., Matsukawa, K., Adachi, M., Tomaru, Y., Ishikawa, K., Hotta, J. 2023 Establishment of a simple method for cryopreservation of the marine diatoms, Chaetoceros and Phaeodactylum. J. Appl. Phycol., 33, 2285–2293.
Keywords: CELLBANKER; Cryopreservation; Marine diatoms; Chaetoceros tenuissimus; Phaeodactylum tricornutum; Transgenic diatom
Strain(s): 324,  3714,  3715 
DOI: 10.1007/s10811-023-03040-w

Tomaru, Y. 2025 Kinetics of adsorption of two distinct viruses to marine planktonic diatom Chaetoceros tenuissimus Meunier. Plankton Benthos Res., 20, 142-147.
Keywords: adsorption kinetics; Chaetoceros tenuissimus; CtenDNAV-II; CtenRNAV-II; diatom
Strain(s): 3713,  3714,  3715 
DOI: 10.3800/pbr.20.142

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