Strain number NIES-3773  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pyrobotrys squarrosa (Korshikov) Korshikov  
Synonym Chlamydobotrys squarrosa Korshikov 1928; Uva squarrosa (Korshikov) Fott 1967; Pyrobotrys squarrosa (Korshikov) Huber-Pestalozzi in Fott 1967  
Former name  
Common name Green alga  
Locality (Date of collection) Akiragi, Hagi, Yamaguchi, Japan (2013-01-08)  
Latitude / Longitude 34.347111 / 131.419667 
Habitat (Isolation source) Freshwater (Sediment)  
History < Sugasawa, Mizuho  
Isolator (Date of isolation) Sugasawa, Mizuho (2014-04-08)  
Identified by Sugasawa, Mizuho  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  AFAC  
Temperature:  20 C
Light intensity:  12-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 18S rRNA ( LC093474 ) , rbcL ( LC093475 ) , psaB ( LC093476 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Other strain no. Other strain no. : YS11  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2564
Pyrobotrys squarrosa Green alga   Freshwater (Paddy soil) Mixotrophic ; Osmotrophic ; Resting spore-forming  
NIES-3770
Pyrobotrys casinoensis Green alga   Freshwater (Sediment) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3771
Pyrobotrys elongata Green alga   Freshwater (Paddy soil) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3772
Pyrobotrys elongata Green alga   Freshwater (Paddy soil) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3774
Pyrobotrys squarrosa Green alga   Freshwater (Sediment) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-3769
Pyrobotrys casinoensis Green alga   Freshwater (Sediment) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Reference
Sugasawa, M., Matsuzaki, R., Kawafune, K., Takahashi, T., Kawachi, M., Krientiz, L., Nozaki, H. 2015 Taxonomic study of Pyrobotrys (Spondylomoraceae, Chlorophyceae) based on comparative morphological and molecular analyses of culture strains established using novel methods. Cytologia, 80, 513-524.
Keywords: Molecular phylogeny; Morphology; Pyrobotrys; Pyrobotrys elongata; Spondylomoraceae; Stigma; Taxonomy; Ultrastructure
Strain(s): 3768376937703771377237733774 
DOI: 10.1508/cytologia.80.513

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