Strain Data

Strain number		NIES-230
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Merismopedia tenuissima Lemmermann
Synonym
Former name
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Tsukuba, Ibaraki, Japan (1984-05-07)
Latitude / Longitude		36.105319 / 140.081738
Habitat (Isolation source)		Freshwater (Pond water)
History		< Kasai, Fumie
Isolator (Date of isolation)		Kasai, Fumie (1984-05-09)
Identified by		Watanabe, Makoto M.
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 15-22 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information
Cell size (min - max)		- 3 μm
Organization
Characteristics
Other strain no.		Other strain no. : F98-2
Remarks		Cryopreserved
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-3779	Merismopedia sp.	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)
NIES-4384	Merismopedia sp.	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-300	Pediastrum angulosum	Green alga	Freshwater (Lake water)
NIES-564	Astrephomene perforata	Green alga	Freshwater (Paddy soil)	Phototaxis ; Authentic strain ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-565
NIES-132	Coelastrum reticulatum	Green alga	Freshwater
NIES-530	Stichococcus bacillaris	Green alga	Freshwater (River water)	Starch production (Shimonaga, T. 2008)
NIES-28	Desmonostoc sp.	Blue-green alga ; Cyanobacteria

Reference
Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159. Strain(s): 41, 45, 81, 102, 204, 230, 298, 592 PubMed: 12715875 DOI: 10.7164/antibiotics.56.154 Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83. Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298 DOI: 10.1016/0269-7491(93)90180-V Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657. Strain(s): 45, 81, 102, 230, 298, 592 PubMed: 14513910 DOI: 10.7164/antibiotics.56.655 Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese). Strain(s): 73, 81, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 204, 230, 298, 299, 604 Hamana, K., Kobayashi, M., Yokota, A., Sekiguchi, H., Niitsu, M. 2008 Cellular polyamine profiles in cyanobacteria. Microbiol. Cult. Coll., 24, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine Strain(s): 26, 33, 36, 46, 206, 208, 230, 267, 268, 305, 361, 416, 433, 434, 481, 507, 597, 598, 931, 932, 1031, 2115, 2133, 2135 Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116 DOI: 10.1016/j.hal.2012.01.003 Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303. Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate Strain(s): 35, 119, 227, 230, 2235 PubMed: 21906932 DOI: 10.1016/j.biortech.2011.07.101 Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A. 2006 Evaluation of herbicide effects on microalgal cells by flow cytometric analysis. J. Weed Sci. Tech., 51, 239-248 (in Japanese with English summary). Keywords: herbicide; flow cytometry; algae; morphological change; recoverability; ecological risk assessment Strain(s): 71, 230 DOI: 10.3719/weed.51.239 Kim, B.-H., Oh, H.-M., Lee, Y.-K., Choi, G.-G., Ahn, C.-y., Yoon, B.-D., Kim, H.-S., 2006 Simple method for RNA preparation from cyanobacteria. J. Phycol., 42, 1137-1141. Keywords: bead–phenol–chloroform method; cyanobacteria; RNA extraction; xanthogenate–SDS–phenol method Strain(s): 33, 39, 230 DOI: 10.1111/j.1529-8817.2006.00263.x Baresova, M., Pivokonsky, M., Novotna, K., Naceradska, J., Branyik, T. 2017 An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Res., 122, 70-77. Keywords: Algal cellular organic matter; Coagulation; Cyanobacterial cells; Merismopedia tenuissima; Water treatment Strain(s): 230 PubMed: 28591663 DOI: 10.1016/j.watres.2017.05.070 Sun, L. W., Jiang, W. J.4, Zhang, J. Y., Wang, W. Q., Du, Y., Sato, H., Kawachi, M., Yu, R. 2018 Identification and detection sensitivity of Microcystis aeruginosa from mixed and field samples using MALDI-TOF MS. Environ. Monit. Assess., 190, 712 (article ID). Keywords: Microcystis aeruginosa; MALDI-TOFMS; Cyanobacterial bloom; Ribosomal protein Strain(s): 206, 230, 843, 1639 PubMed: 30415457 DOI: 10.1007/s10661-018-7093-5 Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148

Reference

Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159.
Strain(s): 41, 45, 81, 102, 204, 230, 298, 592
PubMed: 12715875
DOI: 10.7164/antibiotics.56.154

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35, 44, 73, 77, 102, 137, 151, 230, 231, 255, 275, 280, 298
DOI: 10.1016/0269-7491(93)90180-V

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657.
Strain(s): 45, 81, 102, 230, 298, 592
PubMed: 14513910
DOI: 10.7164/antibiotics.56.655

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 73, 81, 87, 88, 89, 90, 91, 98, 99, 100, 101, 102, 104, 105, 106, 108, 109, 110, 111, 112, 204, 230, 298, 299, 604

Hamana, K., Kobayashi, M., Yokota, A., Sekiguchi, H., Niitsu, M. 2008 Cellular polyamine profiles in cyanobacteria. Microbiol. Cult. Coll., 24, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 26, 33, 36, 46, 206, 208, 230, 267, 268, 305, 361, 416, 433, 434, 481, 507, 597, 598, 931, 932, 1031, 2115, 2133, 2135

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116
DOI: 10.1016/j.hal.2012.01.003

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303.
Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 35, 119, 227, 230, 2235
PubMed: 21906932
DOI: 10.1016/j.biortech.2011.07.101

Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A. 2006 Evaluation of herbicide effects on microalgal cells by flow cytometric analysis. J. Weed Sci. Tech., 51, 239-248 (in Japanese with English summary).
Keywords: herbicide; flow cytometry; algae; morphological change; recoverability; ecological risk assessment
Strain(s): 71, 230
DOI: 10.3719/weed.51.239

Kim, B.-H., Oh, H.-M., Lee, Y.-K., Choi, G.-G., Ahn, C.-y., Yoon, B.-D., Kim, H.-S., 2006 Simple method for RNA preparation from cyanobacteria. J. Phycol., 42, 1137-1141.
Keywords: bead–phenol–chloroform method; cyanobacteria; RNA extraction; xanthogenate–SDS–phenol method
Strain(s): 33, 39, 230
DOI: 10.1111/j.1529-8817.2006.00263.x

Baresova, M., Pivokonsky, M., Novotna, K., Naceradska, J., Branyik, T. 2017 An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Res., 122, 70-77.
Keywords: Algal cellular organic matter; Coagulation; Cyanobacterial cells; Merismopedia tenuissima; Water treatment
Strain(s): 230
PubMed: 28591663
DOI: 10.1016/j.watres.2017.05.070

Sun, L. W., Jiang, W. J.4, Zhang, J. Y., Wang, W. Q., Du, Y., Sato, H., Kawachi, M., Yu, R. 2018 Identification and detection sensitivity of Microcystis aeruginosa from mixed and field samples using MALDI-TOF MS. Environ. Monit. Assess., 190, 712 (article ID).
Keywords: Microcystis aeruginosa; MALDI-TOFMS; Cyanobacterial bloom; Ribosomal protein
Strain(s): 206, 230, 843, 1639
PubMed: 30415457
DOI: 10.1007/s10661-018-7093-5

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.