Strain Data

A big image opens when the thumbnail is clicked.(nies-0252.jpg)
Strain number NIES-252  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis astigmatica Inouye & Pienaar  
Synonym  
Former name  
Common name  
Locality (Date of collection) Tateyama Port, Chiba, Japan (1983-08-10)  
Latitude / Longitude 34.998 / 139.842 
Habitat (Isolation source) Marine (Seawater)  
History < Inouye, Isao  
Isolator (Date of isolation) Inouye, Isao (1983-08-10)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition) 		Medium:  f/2 ;  ESM  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information Plastid genome ( KJ746600 ) , 18S rRNA ( AB158374 FN562433 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563084 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610145 )  
Cell size (min - max) - 8 μm  
Organization Unicellular; Flagellate 
Characteristics Red tide ; Genome decoded strain (Lemieux et al. 2014)  
Other strain no. Other strain no. : 810-13  
Remarks Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-1418
 		Nephroselmis sp.   Marine (Seawater)  
NIES-486
 		Nephroselmis viridis   Marine (Sediment) Red tide ; Authentic strain (Yamaguchi et al. 2011)  
NIES-483
 		Nephroselmis olivacea   Freshwater (Pond sediment) Heterothallic ; Mating type (+)  
NIES-484
 		Nephroselmis olivacea   Freshwater (Pond sediment) Genome decoded strain (Turmel et al. 1999a, Turmel et al. 1999b) ; Heterothallic ; Mating type (−)  
NIES-1415
 		Nephroselmis astigmatica   Marine (Sand and seawater) Benthic  
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-934
 		Nephroselmis spinosa   Marine (Sediment) , Euryhaline  
NIES-1416
 		Nephroselmis pyriformis   Marine (Seawater)  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-381
 		Eutreptiella gymnastica   Marine Red tide ; Genome decoded strain  
NIES-1411
 		Micromonas commoda   Marine (Seawater)  
NIES-360
 		Oltmannsiellopsis viridis   Marine (Seawater) Genome decoded strain (Pombert, J. F. et al. 2006a, Pombert, J. F. et al. 2006b)  
NIES-254
 		Pyramimonas parkeae   Marine (Seawater) Red tide ; Prey for Oxyrrhis marina NIES-494 and NIES-3607  
NIES-221
 		Pterosperma cristatum   Marine (Sediment) Red tide  
Reference
Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 252,  483,  486,  934,  935,  2309 
DOI: 10.1111/j.1529-8817.2005.00103.x

Nakayama, T., Suda, S., Kawachi, M., Inouye, I. 2007 Phylogeny and ultrastructure of Nephroselmis and Pseudoscourfieldia (Chlorophyta), including the description of Nephroselmis anterostigmatica sp. nov. and a proposal for the Nephroselmidales ord. nov. Phycologia, 46, 680-697.
Keywords: 18S rDNA; hair scales; Nephroselmidales ord nov.; Nephroselmis anterostigmatica sp. nov.; phylogeny; Prasinophyceae; Pseudoscourfieldia marina; taxonomy; ultrastructure
Strain(s): 252,  935 
DOI: 10.2216/04-25.1

Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classification of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252,  483 
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720.
Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa
Strain(s): 1,  3,  7,  221,  251,  252,  254,  359,  381,  462,  486 
DOI: 10.1007/s10811-007-9257-5

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252,  255,  360,  483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Lemieux, C., Otis, C.,Turmel, M. 2014 Six newly sequenced chloroplast genomes from prasinophyte green algae provide insights into the relationships among prasinophyte lineages and the diversity of streamlined genome architecture in picoplanktonic species. BMC Evol. Biol., 14, 857 (article ID).
Keywords: Viridiplantae; Prasinophytes; Prasinococcales; Picocystis; Picoplanktonic algae; Nephroselmis; Plastid genome; Phylogenomics; Genome reduction; trans-spliced group II intron
Strain(s): 252 
PubMed: 25281016
DOI: 10.1186/1471-2164-15-857

Lepere, C., Demura, M., Kawachi, M., Romac, S., Probert, I., Vaulot, D. 2011 Whole-genome amplification (WGA) of marine photosynthetic eukaryote populations. FEMS Microbiol. Ecol., 76, 513-523.
Keywords: whole-genome amplification; photosynthetic; microbial eukaryote; flow cytometry sorting; rRNA genes
Strain(s): 252,  1411 
PubMed: 21348885
DOI: 10.1111/j.1574-6941.2011.01072.x

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