| Strain number | NIES-4000 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Chlorophyceae | |||
| Scientific name | Medakamo hakoo T.Kuroiwa | |||
| Synonym | ||||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | Kagurazaka, Shinjuku-ku, Tokyo, Japan (2013-09-11) | |||
| Latitude / Longitude | 35.703 / 139.738 | |||
| Habitat (Isolation source) | Freshwater (Moat water) | |||
| History | < Inui, Yayoi; Matsunaga, Sachihiro; Kuroiwa, Tsuneyoshi | |||
| Isolator (Date of isolation) | Inui, Yayoi; Matsunaga, Sachihiro (2015-10-20) | |||
| Identified by | Kuroiwa, Tsuneyoshi; Nozaki, Hisayoshi | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic | |||
|
Culture condition (Preculture condition) |
Medium:
C
Temperature: 20 C Light intensity: 6-10 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 4 M |
|||
| Gene information | Whole-genome ( CP089450-CP089465 ) , Mitochondrial genome ( LC604817 ) , Plastid genome ( LC604816 ) | |||
| Cell size (min - max) | - 1 μm | |||
| Organization | Unicellular | |||
| Characteristics | Genome decoded strain (Kato et al. 2023) ; Type specimen (NIES-50025, Holotype) | |||
| Other strain no. | Other strain no. : Medakamo hakoo 311; M. hakoo 311 | |||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
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Kuroiwa, T., Ohnuma, M., Nozaki, H., Imoto, Y., Misumi, O., Kuroiwa, H.
2015
Cytological evidence of cell-nuclear genome size of a new ultra-small unicellular freshwater green alga, “Medakamo hakoo” strain M-hakoo 311 I. Comparison with Cyanidioschyzon merolae and Ostreococcus tauri.
Cytologia,
80,
143-150.
Keywords: Ultra-small alga; Chlorophyte M. hakoo; Cyanidioschyzon merolae; Ostreococcus tauri; Genome size; Cytochemistry Strain(s): 2673, 3377, 4000 DOI: 10.1508/cytologia.80.143 Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H. 2016 Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′,6-diamidino-2-phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis. Cytologia, 81, 69-76. Keywords: Ultrasmall genome size; Medakamo hakoo; Saccharyomyces cerevisiae; Chlorella variabilis; DAPI staining; Microwave oven Strain(s): 3377, 4000 DOI: 10.1508/cytologia.81.69 Kato, S., Misumi, O., Maruyama, S., Nozaki, H., Tsujimoto-Inui, Y., Takusagawa, M., Suzuki, S., Kuwata, Noda, S., Ito, N., Okabe, Y., Sakamoto, Yagisawa, Y., Matsunaga, M. T., Matsubayashi, Y., Yamaguchi, H., Kawachi, M., Kuroiwa, H., Kuroiwa, T., Matsunaga, S. 2023 Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo. Commun. Biol., 6, 89 (article ID). Strain(s): 4000 PubMed: 36690657 DOI: 10.1038/s42003-022-04367-9 Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H, Kuroiwa, T., Matsunaga, S. 2023 Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo. Genes Genet. Syst., 98, 353-360. Keywords: mitochondria; chloroplast; organelle genome; Trebouxiophyceae Strain(s): 4000 PubMed: 38267054 DOI: 10.1266/ggs.23-00275 |
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