A big image opens when the thumbnail is clicked.(nies-4000.jpg)
Strain number NIES-4000  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Medakamo hakoo T.Kuroiwa  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Kagurazaka, Shinjuku-ku, Tokyo, Japan (2013-09-11)  
Latitude / Longitude 35.703 / 139.738 
Habitat (Isolation source) Freshwater (Moat water)  
History < Inui, Yayoi; Matsunaga, Sachihiro; Kuroiwa, Tsuneyoshi  
Isolator (Date of isolation) Inui, Yayoi; Matsunaga, Sachihiro (2015-10-20)  
Identified by Kuroiwa, Tsuneyoshi; Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  6-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information Whole-genome ( CP089450-CP089465 ) , Mitochondrial genome ( LC604817 ) , Plastid genome ( LC604816 )  
Cell size (min - max) - 1 μm  
Organization Unicellular 
Characteristics Genome decoded strain (Kato et al. 2023) ; Type specimen (NIES-50025, Holotype)  
Other strain no. Other strain no. : Medakamo hakoo 311; M. hakoo 311  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Kuroiwa, T., Ohnuma, M., Nozaki, H., Imoto, Y., Misumi, O., Kuroiwa, H. 2015 Cytological evidence of cell-nuclear genome size of a new ultra-small unicellular freshwater green alga, “Medakamo hakoo” strain M-hakoo 311 I. Comparison with Cyanidioschyzon merolae and Ostreococcus tauri. Cytologia, 80, 143-150.
Keywords: Ultra-small alga; Chlorophyte M. hakoo; Cyanidioschyzon merolae; Ostreococcus tauri; Genome size; Cytochemistry
Strain(s): 267333774000 
DOI: 10.1508/cytologia.80.143

Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H. 2016 Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′,6-diamidino-2-phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis. Cytologia, 81, 69-76.
Keywords: Ultrasmall genome size; Medakamo hakoo; Saccharyomyces cerevisiae; Chlorella variabilis; DAPI staining; Microwave oven
Strain(s): 33774000 
DOI: 10.1508/cytologia.81.69

Kato, S., Misumi, O., Maruyama, S., Nozaki, H., Tsujimoto-Inui, Y., Takusagawa, M., Suzuki, S., Kuwata, Noda, S., Ito, N., Okabe, Y., Sakamoto, Yagisawa, Y., Matsunaga, M. T., Matsubayashi, Y., Yamaguchi, H., Kawachi, M., Kuroiwa, H., Kuroiwa, T., Matsunaga, S. 2023 Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo. Commun. Biol., 6, 89 (article ID).
Strain(s): 4000 
PubMed: 36690657
DOI: 10.1038/s42003-022-04367-9

Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H, Kuroiwa, T., Matsunaga, S. 2023 Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo. Genes Genet. Syst., 98, 353-360.
Keywords: mitochondria; chloroplast; organelle genome; Trebouxiophyceae
Strain(s): 4000 
PubMed: 38267054
DOI: 10.1266/ggs.23-00275

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