A big image opens when the thumbnail is clicked.(nies-4017.jpg)
Strain number NIES-4017  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene gubernaculifera Pocock  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Yokota, Sodegaura, Chiba, Japan (2014-07-21)  
Latitude / Longitude 35.389750 / 140.026722 
Habitat (Isolation source) Freshwater (Sediment)  
History < Yamashita, Shota; Nozaki, Hisayoshi  
Isolator (Date of isolation) Yamashita, Shota (2014-10-02)  
Identified by Yamashita, Shota  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  14 D  
Gene information Whole-genome ( BMAR00000000 ) , rbcL ( LC188997 )  
Cell size (min - max) 8 - 20 μm  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Heterotrophic ; Phototaxis ; Genome decoded strain (Yamashita et al. 2021) ; Heterothallic ; Isogamous ; Haploid life cycle ; Resting spore-forming  
Other strain no. Other strain no. : 2014-1002-YkAs8  
Remarks Cryopreserved 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-855
Astrephomene gubernaculifera Green alga   (Soil) Mixotrophic ; Phototaxis ; Heterothallic  
NIES-564
Astrephomene perforata Green alga   Freshwater (Paddy soil) Phototaxis ; Authentic strain ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-565  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-432
Carteria radiosa Green alga   Freshwater (Pond water)  
NIES-728
Platydorina caudata Green alga   Freshwater (Pond water) Phototaxis  
NIES-566
Basichlamys sacculifera Green alga   Freshwater (Pond sediment) Phototaxis ; Single celled  
NIES-875
Vitreochlamys aulata Green alga   Freshwater (Paddy soil) Phototaxis  
NIES-153
Desmotetra delicata Green alga   Terrestrial (Soil) Authentic strain (Watanabe 1983, Watanabe et al. 2006)  
Reference
Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID).
Strain(s): 40174018 
PubMed: 27829356
DOI: 10.1186/s12862-016-0794-x

Yamashita, S., Yamamoto, K., Matsuzaki, R., Shigekatsu, S., Yamaguchi, H., Hirooka, S., Minakuchi, Y., Miyagishima, S., Masanobu, M., Atsushi, T., Hisayoshi, N. 2021 Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation. Sci Rep, 11, 22231 (article ID).
Strain(s): 4017 
PubMed: 34811380
DOI: 10.1038/s41598-021-01521-x

Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Chen, Y., Xiong, Q., Dai, Q., Liu, G. 2025 Six newly sequenced chloroplast genomes from quadriflagellate Chlamydomonadales (Chlorophyceae): Phylogeny and comparative genome analyses. Genome Biol. Evol., 17, evaf074 (article ID).
Keywords: quadriflagellate chlamydomonadaleans; phylogenomics; Corbierea; Strain(s): 15325742543247456672887513634017 
PubMed: 40247659
DOI: 10.1093/gbe/evaf074

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