Strain number NIES-4021  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox sagami Nozaki & N.Ueki  
Synonym  
Former name Formerly identified as Volvox sp. Sagami, named based on Nozaki et al. 2026[2026 June]  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2013-06-14)  
Latitude / Longitude 35.6093 / 139.188 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2013-06-14)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2024 Jul]  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS1-5.8S-ITS2 ( LC191308 ) , rbcL ( LC191316 ) , psbC ( LC191326 ) , atc1 ( LC818981 )  
Cell size (min - max) 10 - 60 μm  
Organization Colonial; Flagellate 
Characteristics Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
Other strain no. Other strain no. : 13-614-Vx12  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-732
Volvox carteri Green alga ; Volvocine alga   Freshwater (Paddy soil) Phototaxis ; Authentic strain ; Heterothallic ; Dioecious ; Oogamy ; Female ; Crosses with NIES-733  
NIES-3780
Volvox africanus Green alga ; Volvocine alga   Freshwater (Lake water) Phototaxis ; Homothallic ; Monoecious with male ; Oogamy ; F1 strain (NIES-3784) ; Genome decoded strain (Yamamoto et al. 2021)  
NIES-865
Volvox carteri Green alga ; Volvocine alga   Phototaxis ; Heterothallic ; Dioecious ; Oogamy ; Female  
NIES-4029
Volvox ferrisii Green alga ; Volvocine alga   Freshwater (Pond water) Phototaxis ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming  
NIES-867
Volvox gigas Green alga ; Volvocine alga   Phototaxis ; Heterothallic ; Dioecious ; Oogamy ; Female  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-545
Volvulina steinii Green alga   Freshwater (Paddy soil) Phototaxis ; Heterothallic ; Mating type (−) ; Crosses with NIES-546  
NIES-4465
Volvox ferrisii Green alga ; Volvocine alga   Freshwater (Paddy soil) Phototaxis ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming ; Life cycle (Isaka et al. 2012, J. Phycol.)  
NIES-4024
Volvox sp. Sagami Green alga ; Volvocine alga   Freshwater (Lake water) Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
NIES-852
Gonium octonarium Green alga   Phototaxis  
NIES-3988
Volvox ferrisii Green alga ; Volvocine alga   Freshwater (Pond water) Mixotrophic ; Phototaxis ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming ; Life cycle (Isaka et al. 2012, J. Phycol.)  
Reference
Nozaki, H., Ueki, N., Isaka, N., Saigo, T., Yamamoto, K., Matsuzaki, R., Takahashi, F., Wakabayashi, K-I., Kawachi, M. 2016 A new morphological type of Volvox from japanese large lakes and recent divergence of this type and V. ferrisii in two different freshwater habitats. PLoS One, 11, e0167148 (article ID).
Strain(s): 273627373987398840214022402340244025402640274028 
PubMed: 27880842
DOI: 10.1371/journal.pone.0167148

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2024 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Matsuzaki, R., Shimotori, K., Ueki, N., Heman, W., Mahakham, W., Yamaguchi, H., Tanabe, Y., Kawachi, M. 2024 Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa. PLoS One, 19, e0310549 (article ID).
Strain(s): 543273627403874402140224258425943364337443446614662 
PubMed: 39312548
DOI: 10.1371/journal.pone.0310549

Nozaki, H., Matsuzaki, R., Takahashi, K., Yamamoto, K., Suzuki, S., Ootsuki, R., Kawachi, M., Tanabe, Y., Ueki, N. 2026 Molecular and morphological delineations of two homothallic monoicous species, Volvox sagami sp. nov. and V. ferrisii (Volvocales, Chlorophyceae) in Japan. Phycologia, ,
Keywords: Chlorophyta; nuclear genes phylogeny; reproductive isolation; sexual reproduction; Taxonomyvol; vocine algae
Strain(s): 2736273727393987398840214022402640294465453445364537459346614662 
DOI: 10.1080/00318884.2026.2683158

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