Strain number NIES-4033  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Carolibrandtia ciliaticola (Hoshina, Kobayashi, Suzaki & Kusuoka) Hoshina & Nakada  
Synonym Brandtia ciliaticola Hoshina, Kobayashi, Suzaki & Kusuoka  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Biwa, Oroshimo, Kusatsu, Shiga, Japan (2015-12-22)  
Latitude / Longitude 35.0741068 / 135.9317646 
Habitat (Isolation source) Freshwater (Pelagodileptus trachelioides)  
History < Hoshina, Ryo  
Isolator (Date of isolation) Hoshina, Ryo (2016-05-30)  
Identified by Hoshina, Ryo  
State of strain Cryopreservation; Unialgal; Non-axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  CAFAC  
Temperature:  20 C
Light intensity:  14-18 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S-ITS1-5.8S-ITS2 ( LC228604 )  
Cell size (min - max) 3 - 5 μm  
Organization  
Characteristics Symbiotic ; Authentic strain (Hoshina et al. 2018) ; Type specimen (NIES-50018, Holotype)  
Other strain no. Other strain no. : 1224PelagoG  
Remarks Cryopreserved 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2167
Didymogenes sphaerica Green alga   Authentic strain (Hoshina et al. 2013)  
NIES-2173
Chlorella sorokiniana Green alga   Starch production (Miyachi et al. 1986)  
NIES-2330
Didymogenes soliella Green alga   Freshwater (Pond sediment) Authentic strain (Hoshina et al. 2013)  
NIES-4292
Choricystis krienitzii Green alga   Freshwater (Lake water)  
NIES-455
Micractinium bornhemiensis Green alga   Freshwater (Pond sediment)  
Reference
Hoshina, R., Kobayashi, M., Suzaki, T., Kusuoka, Y. 2018 Brandtia ciliaticola gen. et sp. nov. (Chlorellaceae, Trebouxiophyceae) a common symbiotic green coccoid of various ciliate species. Phycol. Res., 66, 76-81.
Keywords: Chlorella; genus definition; rRNA structure
Strain(s): 403240334034 
DOI: 10.1111/pre.12194

Hoshina, R. & Nakada, T. 2018 Carolibrandtia nom. nov. as a replacement name for Brandtia Hoshina (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 66, 82-83.
Keywords: Chlorella-like alga; ciliate; later homonym; nomenclature; photobiont; symbiosis
Strain(s): 403240334034 
DOI: 10.1111/pre.12208

Pröschold, T., Pitsch, G., Darienko, T. 2020 Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a new endosymbiont isolated from ciliates. Diversity-Basel, 12, 200 (article ID).
Keywords: Micractinium tetrahymenae; Tetrahymena; Utricularia; facultative endosymbiosis; ciliate-algae symbiosis
Strain(s): 216721714033 
DOI: 10.3390/d12050200

Pröschold, T. & Darienko, T. 2020 Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges. Symbiosis, 82, 175-188.
Keywords: Choricystis; Lewiniosphaera; Freshwater sponges; Endosymbiosis
Strain(s): 21672171234240334292 
PubMed: 33328698
DOI: 10.1007/s13199-020-00711-x

Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190.
Keywords: CBC approach; delimitation algorithms; green microalgae, morphology; taxonomy
Strain(s): 4552167217121732330403240334034 
DOI: 10.1111/pre.12551

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