Strain number NIES-4066  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pleodorina sphaerica M.O.P.Iyengar  
Synonym Eudorina sphaerica (Iyengar) Goldstein 1964  
Former name  
Common name Green alga  
Locality (Date of collection) Phon Thong Lake, Phon Thong District, Roi Et Province, Thailand (2015-11-28)  
Latitude / Longitude 16.27869444 / 104.00000556 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2015-11-28)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  AF-6/3  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information psaA ( LC215637 ) , atpB ( LC215636 ) , psbC ( LC215635 ) , rbcL ( LC215634 ) , psaB ( LC215633 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Dioecious  
Other strain no. Other strain no. : 2015-1128-2P-12  
Remarks Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-1363
Pleodorina starrii Green alga   Freshwater (Lake water) Other water bloom ; Phototaxis ; Authentic strain (Nozaki et al. 2006) ; Heterothallic ; Anisogamous ; Male ; Genome decoded strain (Takahashi et al. 2003;( Smith et al. 2013)  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-4480
Pleodorina starrii Green alga   Freshwater (Reservoir water) Phototaxis ; Unisexual male  
NIES-4670
Pleodorina japonica Green alga   Freshwater (River water) Phototaxis ; Homothallic ; Dioecious  
NIES-4481
Pleodorina starrii Green alga   Freshwater (Reservoir water) Phototaxis ; Unisexual female ; Genome decoded strain (Takahashi et al. 2003)  
NIES-3780
Volvox africanus Green alga ; Volvocine alga   Freshwater (Lake water) Phototaxis ; Homothallic ; Monoecious with male ; Oogamy ; F1 strain (NIES-3784) ; Genome decoded strain (Yamamoto et al. 2021)  
NIES-541
Volvox aureus Green alga ; Volvocine alga   Freshwater (Sediment) Phototaxis  
Reference
Nozaki. H., Mahakham, W., Athibai, S., Yamamoto, K., Takusagawa, M., Misumi, O., Herron, M. D., Rosenzweig, F., Kawachi, M. 2017 Rediscovery of the species of ‘ancestral Volvox’: morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. Phycologia, 56, 469-475.
Keywords: Molecular phylogeny; Morphology; Pleodorina; Pleodorina sphaerica; Sexuality; Somatic cell; Volvox
Strain(s): 4066406740684069 
PubMed: 29375162
DOI: 10.2216/17-3.1

Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T., Nozaki, H. 2021 Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system. Evolution, 75, 2984-2993.
Keywords: Haploid species; inbreeding; life-history evolution; mating systems; reproductive isolation; sex
Strain(s): 456460541544731732872891892136213632569338233833780378240664068447944804481448244834484 
PubMed: 34250602
DOI: 10.1111/evo.14306

Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamaguchi, H., Kawachi, K., Tanabe, Y. 2026 Cryopreservation of Pleodorina (Volvocales, Chlorophyceae) and its rickettsialendosymbionts. Phycologia, 65, 63-68.
Keywords: Cryoprotectant; Endosymbiotic bacteria; Pleodorina japonica; Sexualreproduction; Volvocinealgae
Strain(s): 7361362406646704672 
DOI: 10.1080/00318884.2026.2624407

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