Strain number NIES-4068  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox tertius Art.Meyer  
Synonym Merillosphaera tertia (Art.Meyer) W.R.Shaw 1922; Heterotypic synonyms; Volvox bernardii Playfair null; Volvox mononae G.M.Smith 1920; Merrillosphaera migulae W.R.Shaw 1922  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Ninepipe Reservoir, Montana, USA (2014-07-28)  
Latitude / Longitude 47.45541667 /  
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi < Yamamoto, Kayoko  
Isolator (Date of isolation) Yamamoto, Kayoko (2014-07-28)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 May]  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  23 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information rbcL ( LC215631 ) , atpB ( LC215630 ) , psab ( LC215629 ) , psaA ( LC215628 ) , psbC ( LC215627 ) , ITS1-5.8S-ITS2 ( LC215411 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Dioecious  
Other strain no. Other strain no. : 1-3 k-4  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-867
Volvox gigas Green alga ; Volvocine alga   Phototaxis ; Heterothallic ; Dioecious ; Oogamy ; Female  
NIES-891
Volvox aureus Green alga ; Volvocine alga   Freshwater (Lake water) Phototaxis  
NIES-3780
Volvox africanus Green alga ; Volvocine alga   Freshwater (Lake water) Phototaxis ; Homothallic ; Monoecious with male ; Oogamy ; F1 strain (NIES-3784) ; Genome decoded strain (Yamamoto et al. 2021)  
NIES-693
Volvox aureus Green alga ; Volvocine alga   Freshwater (Stream water) Other water bloom ; Phototaxis ; Homothallic ; Dioecious ; Oogamy  
NIES-4270
Volvox dissipatrix Green alga ; Volvocine alga   Freshwater (Bog water) Phototaxis ; Homothallic ; Monoecious  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-3383
Colemanosphaera charkowiensis Green alga   Freshwater (Pond water) Planktonic  
NIES-732
Volvox carteri Green alga ; Volvocine alga   Freshwater (Paddy soil) Phototaxis ; Authentic strain ; Heterothallic ; Dioecious ; Oogamy ; Female ; Crosses with NIES-733  
NIES-4480
Pleodorina starrii Green alga   Freshwater (Reservoir water) Phototaxis ; Unisexual male  
NIES-2569
Volvox ovalis Green alga ; Volvocine alga   Freshwater (Water) Phototaxis ; Authentic strain  
NIES-1362
Pleodorina starrii Green alga   Freshwater (Lake water) Other water bloom ; Phototaxis ; Heterothallic ; Anisogamous ; Female  
Reference
Nozaki. H., Mahakham, W., Athibai, S., Yamamoto, K., Takusagawa, M., Misumi, O., Herron, M. D., Rosenzweig, F., Kawachi, M. 2017 Rediscovery of the species of ‘ancestral Volvox’: morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. Phycologia, 56, 469-475.
Keywords: Molecular phylogeny; Morphology; Pleodorina; Pleodorina sphaerica; Sexuality; Somatic cell; Volvox
Strain(s): 4066406740684069 
PubMed: 29375162
DOI: 10.2216/17-3.1

Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T., Nozaki, H. 2021 Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system. Evolution, 75, 2984-2993.
Keywords: Haploid species; inbreeding; life-history evolution; mating systems; reproductive isolation; sex
Strain(s): 456460541544731732872891892136213632569338233833780378240664068447944804481448244834484 
PubMed: 34250602
DOI: 10.1111/evo.14306

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