Strain Data

Strain number		NIES-534
Phylum		Heterokontophyta
Class		Bacillariophyceae
Scientific name		Thalassionema nitzschioides (Grunow) Hustedt
Synonym
Former name
Common name		Pennate diatom
Locality (Date of collection)		Matoya Bay, Mie, Japan (1984-09-01)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< Sawaguchi, Tomohiro
Isolator (Date of isolation)		Sawaguchi, Tomohiro (1984-09-13)
Identified by		Sawaguchi, Tomohiro
State of strain		Subculture; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: f/2 Temperature: 15 C Light intensity: 12-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 42 D
Gene information		atub ( AB766059 ) , EF-1A mRNA ( AB766032 )
Cell size (min - max)		- 29 μm
Organization		Unicellular
Characteristics
Other strain no.		Other strain no. : MBB-6
Remarks
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-353	Gephyrocapsa oceanica	Coccolithophore	Marine (Seawater)	Planktonic
NIES-553	Chaetoceros socialis	Centric diatom	Marine (Seawater)	Red tide
NIES-697	Rhodomonas acuta		Marine	Authentic strain of Cryptomonas acuta Butcher
NIES-413	Achnanthidium minutissimum	Pennate diatom	Freshwater (River water)
NIES-1834	Gymnodinium catenatum	Dinoflagellate	Marine (Seawater)

Reference
Otsuki, A., Watanabe, M. M., Sugahara, K. 1987 Chlorophyll pigments in methanol extracts from ten axenic cultured diatoms and three green algae as determined by reverse phase HPLC with fluorometric detection. J. Phycol., 23, 406-414. Keywords: chlorophyll c complex; chlorophyll pigments; diatoms; field desorption mass spectrometry; methanol extracts; reverse phase HPLC Strain(s): 16, 56, 71, 157, 225, 534 DOI: 10.1111/j.1529-8817.1987.tb02526.x Kamikawa, R., Sanchez-Perez, G. F., Sako, Y., Roger, A. J., Inagaki, Y. 2009 Expanded phylogenies of canonical and non-canonical types of methionine adenosyltransferase reveal a complex history of these gene families in eukaryotes. Mol. Phylogenet. Evol., 53, 565-570. Keywords: Methionine adenosyltransferase; Lateral gene transfer; Diaoms; Aureococcus; Haptophytes; Cryptomonads Strain(s): 17, 350, 417, 534, 586, 1045, 1349 PubMed: 19577655 DOI: 10.1016/j.ympev.2009.06.016 Kamikawa, R., Brown, M. W., Nishimura, Y., Sako, Y., Heiss, A. A., Yubuki, N., Gawryluk, R., Simpson, A. G. B., Roger, A. J., Hashimoto, T., Inagaki, Y. 2013 Parallel re-modeling of EF-1α function: divergent EF-1α genes co-occur with EFL genes in diverse distantly related eukaryotes. BMC Evol. Biol., 13, 131 (article ID). Keywords: Diatoms; Differential Gene Loss; EF-1α; EFL; Functional Remodeling; Goniomonas; Pythium; Spizellomyces; Thecamonas Strain(s): 417, 534, 1349, 1371, 1373, 1374 PubMed: 23800323 DOI: 10.1186/1471-2148-13-131 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 DOI: 10.60369/microresys.33.1_19 Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID). Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778 DOI: 10.1016/j.algal.2021.102191 Zhou, G.-J., Lai, R. W. S., Yang, Y., Kim, M.-S., Lee, Y. H., Lee, J.-S., Leung, K. M. Y. 2026 Retinoic acids can hinder molting and development of the marine copepod Tigriopus japonicus in the coastal environment. J. Hazard. Mater., 513, 142521 (article ID). Keywords: Retinoids; Algae; Chronic toxicity; Copepod; Molting; Ecdysteroid Strain(s): 1, 5, 12, 223, 237, 323, 377, 534, 586, 601, 675, 682, 1383, 1396, 1834, 2327, 2590 PubMed: 42208294 DOI: 10.1016/j.jhazmat.2026.142521

Reference

Otsuki, A., Watanabe, M. M., Sugahara, K. 1987 Chlorophyll pigments in methanol extracts from ten axenic cultured diatoms and three green algae as determined by reverse phase HPLC with fluorometric detection. J. Phycol., 23, 406-414.
Keywords: chlorophyll c complex; chlorophyll pigments; diatoms; field desorption mass spectrometry; methanol extracts; reverse phase HPLC
Strain(s): 16, 56, 71, 157, 225, 534
DOI: 10.1111/j.1529-8817.1987.tb02526.x

Kamikawa, R., Sanchez-Perez, G. F., Sako, Y., Roger, A. J., Inagaki, Y. 2009 Expanded phylogenies of canonical and non-canonical types of methionine adenosyltransferase reveal a complex history of these gene families in eukaryotes. Mol. Phylogenet. Evol., 53, 565-570.
Keywords: Methionine adenosyltransferase; Lateral gene transfer; Diaoms; Aureococcus; Haptophytes; Cryptomonads
Strain(s): 17, 350, 417, 534, 586, 1045, 1349
PubMed: 19577655
DOI: 10.1016/j.ympev.2009.06.016

Kamikawa, R., Brown, M. W., Nishimura, Y., Sako, Y., Heiss, A. A., Yubuki, N., Gawryluk, R., Simpson, A. G. B., Roger, A. J., Hashimoto, T., Inagaki, Y. 2013 Parallel re-modeling of EF-1α function: divergent EF-1α genes co-occur with EFL genes in diverse distantly related eukaryotes. BMC Evol. Biol., 13, 131 (article ID).
Keywords: Diatoms; Differential Gene Loss; EF-1α; EFL; Functional Remodeling; Goniomonas; Pythium; Spizellomyces; Thecamonas
Strain(s): 417, 534, 1349, 1371, 1373, 1374
PubMed: 23800323
DOI: 10.1186/1471-2148-13-131

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 345, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691
DOI: 10.60369/microresys.33.1_19

Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID).
Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles
Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778
DOI: 10.1016/j.algal.2021.102191

Zhou, G.-J., Lai, R. W. S., Yang, Y., Kim, M.-S., Lee, Y. H., Lee, J.-S., Leung, K. M. Y. 2026 Retinoic acids can hinder molting and development of the marine copepod Tigriopus japonicus in the coastal environment. J. Hazard. Mater., 513, 142521 (article ID).
Keywords: Retinoids; Algae; Chronic toxicity; Copepod; Molting; Ecdysteroid
Strain(s): 1, 5, 12, 223, 237, 323, 377, 534, 586, 601, 675, 682, 1383, 1396, 1834, 2327, 2590
PubMed: 42208294
DOI: 10.1016/j.jhazmat.2026.142521

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