Strain number NIES-4530  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Elliptochloris subsphaerica subsp. subsphaerica (Reisigl) Ettl & G.Gärtner  
Synonym Pseudochlorella subsphaerica Reisigl 1964; Chlorella reisiglii Shin Watanabe 1977  
Former name  
Common name Green alga  
Locality (Date of collection) Yokokawadaiichi Park, Naka-ku, Hiroshima, Hiroshima, Japan (2019-06-19)  
Latitude / Longitude 34.40751 / 132.44864 
Habitat (Isolation source) Terrestrial (Bark (Keyaki))  
History < Masumoto, Hiroshi < Handa, Shinji  
Isolator (Date of isolation) Handa, Shinji (2019-07-22)  
Identified by Masumoto, Hiroshi; Handa, Shinji  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  10-20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( LC660177 ) , 5.8S-ITS2-28S ( LC660444 ) , rbcL ( LC660432 )  
Cell size (min - max) 4 - 15 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other strain no. : Handa-4057-a  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-4525
Elliptochloris subsphaerica Green alga   Terrestrial (The lichen Multiclavula mucida) Symbiotic (lichen)  
NIES-4528
Elliptochloris subsphaerica Green alga   Terrestrial (stone statue)  
NIES-4523
Elliptochloris subsphaerica Green alga   Terrestrial (The lichen Multiclavula vernalis) Symbiotic (lichen) ; Authentic strain (Masumoto et al. 2026) ; Type specimen (NIES-50026, Holotype)  
NIES-4527
Elliptochloris subsphaerica Green alga   Terrestrial (Wooden Fence)  
NIES-4526
Elliptochloris subsphaerica Green alga   Terrestrial (Concrete) Symbiotic (lichen)  
Reference
Masumoto, H., Handa, S., Tanaka, C., Degawa, Y. 2026 Elliptochloris, not Coccomyxa, is the photobiont of three lichenized Multiclavula species in Japan. Symbiosis, , (article ID).
Keywords: Basidiolichen; Cantharellales; Lichen resynthesis; Photobiont; TEM; Trebouxiophyceae
Strain(s): 4523,  4524,  4525,  4526,  4527,  4528,  4529,  4530,  4531,  4532 
DOI: 10.1007/s13199-026-01161-7

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