Strain number NIES-4661  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox biwakoensis Nozaki & H.Yamaguchi  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) offshore from Yanagaseoki of Lake Biwa, Shiga, Japan (2022-10-20)  
Latitude / Longitude 35.0261274 / 135.8673386 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2021-10-21)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  15-21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC817963 ) , psbO ( LC919155 ) , atp2 ( LC919517 ) , act1 ( LC817974 ) , efl ( LC919570 ) , MID ( LC919591 ) , rbcL ( LC817986 ) , psbC ( LC817992 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Endemic to Japan ; Homothallic ; Monoecious  
Other strain no. Other strain no. : 2022-1021-Biwa1  
Remarks  
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-732
Volvox carteri Green alga ; Volvocine alga   Freshwater (Paddy soil) Phototaxis ; Authentic strain ; Heterothallic ; Dioecious ; Oogamy ; Female ; Crosses with NIES-733  
NIES-3780
Volvox africanus Green alga ; Volvocine alga   Freshwater (Lake water) Phototaxis ; Homothallic ; Monoecious with male ; Oogamy ; F1 strain (NIES-3784) ; Genome decoded strain (Yamamoto et al. 2021)  
NIES-865
Volvox carteri Green alga ; Volvocine alga   Phototaxis ; Heterothallic ; Dioecious ; Oogamy ; Female  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-4022
Volvox sagami Green alga ; Volvocine alga   Freshwater (Lake water) Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
NIES-4537
Volvox rousseletii Green alga ; Volvocine alga   Freshwater (Reservoir water) Phototaxis ; Dioecious ; Heterothallic ; Male  
NIES-4021
Volvox sagami Green alga ; Volvocine alga   Freshwater (Lake water) Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
NIES-4026
Volvox sagami Green alga ; Volvocine alga   Freshwater (Pond water) Other water bloom ; Phototaxis ; Endemic to Japan ; Homothallic ; Oogamy ; Haploid life cycle ; Resting zygote forming  
NIES-3988
Volvox ferrisii Green alga ; Volvocine alga   Freshwater (Pond water) Mixotrophic ; Phototaxis ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming ; Life cycle (Isaka et al. 2012, J. Phycol.)  
Reference
Nozaki, H., Matsuzaki, R., Shimotori, K., Ueki, N., Heman, W., Mahakham, W., Yamaguchi, H., Tanabe, Y., Kawachi, M. 2024 Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa. PLoS One, 19, e0310549 (article ID).
Strain(s): 543,  2736,  2740,  3874,  4021,  4022,  4258,  4259,  4336,  4337,  4434,  4661,  4662 
PubMed: 39312548
DOI: 10.1371/journal.pone.0310549

Nozaki, H., Matsuzaki, R., Takahashi, K., Yamamoto, K., Suzuki, S., Ootsuki, R., Kawachi, M., Tanabe, Y., Ueki, N. 2026 Molecular and morphological delineations of two homothallic monoicous species, Volvox sagami sp. nov. and V. ferrisii (Volvocales, Chlorophyceae) in Japan. Phycologia, 65, 189-198.
Keywords: Chlorophyta; nuclear genes phylogeny; reproductive isolation; sexual reproduction; Taxonomyvol; vocine algae
Strain(s): 2736,  2737,  2739,  3987,  3988,  4021,  4022,  4026,  4029,  4465,  4534,  4536,  4537,  4593,  4661,  4662 
DOI: 10.1080/00318884.2026.2683158

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