Strain number NIES-4686  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Botryococcus braunii Kützing  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Kanna Dam, Ginoza, Okinawa, (2004-04-21)  
Latitude / Longitude 26.486896839382194 / 127.9533505190885 
Habitat (Isolation source) Freshwater (Reservoir water)  
History
Isolator (Date of isolation) Tanoi, Takako (2004-06-**)  
Identified by Tanoi, Takako  
State of strain Subculture; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  AF-6MAF-6  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AB677494 )  
Cell size (min - max)  
Organization  
Characteristics oil (hydrocarbon) production (Race B)  
Other strain no. Other strain no. : BOT-22  
Remarks Axenic; Patent strains, may not be used for commercial purposes. 
Movie  
Related strain information
(Large number of orders and same genus or species)
Reference
Tanoi, T., Kawachi, M., Watanabe, M. M. 2014 Iron and glucose effects on the morphology of Botryococcus braunii with assumption on the colony formation variability. J. Appl. Phycol., 26, 1-8.
Keywords: Botryococcus braunii; Chlorophyta; Colony size; Iron-rich; Iron-limited; Glucose
Strain(s): 4686 
DOI: 10.1007/s10811-013-0026-3

Kawachi, M., Tanoi, T., Demura, M., Kaya, K., Watanabe, M. M. 2012 Relationship between hydrocarbons and molecular phylogeny of Botryococcus braunii. Algal Res., 1, 114-119.
Keywords: Botryococcus braunii; Phylogeny; Hydrocarbon; 18S rRNA
Strain(s): 4686 
DOI: 10.1016/j.algal.2012.05.003

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